Affinity of Streptococcal G Protein to Forest Musk Deer (Moschus berezovskii) Serum Immunoglobulin G

Zhao, Wei; Yu, Dong; Cheng, Jin; Luo, Yan; Wang, Yin; Yao, Xueping; Yang, Zexiao; Ren, Ziwei; Wu, Xi

doi:10.7589/2019-09-223

Cited by 3 publications

(3 citation statements)

References 11 publications

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“…Next, the PPI networks of these 49 proteins were established and presented in Figure 6 (organism: Bos taurus, combined interaction score ≥ 0.4) (the solitary proteins have been removed from the network). The detailed protein-protein interaction information and node degrees of the above-mentioned 49 proteins in the PPI networks were displayed in Supplementary Tables 12, 13, respectively. The outcomes suggested that CTNNB1, ITGB1, Annexin A5 (ANXA5), calreticulin (CALR), prothrombin (F2), matrix metalloproteinase-9 (MMP9), platelet endothelial cell adhesion molecule (PECAM1), thrombospondin-1 (THBS1), heat shock protein HSP 90-beta (HSP90AB1), endoplasmin (HSP90B1), integrin alpha-3 (ITGA3), and moesin (MSN) might be the hub proteins in the PPI networks because they had greater node degrees.…”

Section: Screening and Ppi Network Construction Of Dysregulated Prote...mentioning

confidence: 99%

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ITRAQ-based quantitative proteomics analysis of forest musk deer with pneumonia

Tang

Suo²,

Li³

et al. 2022

Front. Vet. Sci.

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Pneumonia can seriously threaten the life of forest musk deer (FMD, an endangered species). To gain a comprehensive understanding of pneumonia pathogenesis in FMD, iTRAQ-based proteomics analysis was performed in diseased (Pne group) lung tissues of FMD that died of pneumonia and normal lung tissues (Ctrl group) of FMD that died from fighting against each other. Results showed that 355 proteins were differentially expressed (fold change ≥ 1.2 and adjusted P-value < 0.05) in Pne vs. Ctrl. GO/KEGG annotation and enrichment analyses showed that dysregulated proteins might play vital roles in bacterial infection and immunity. Given the close association between bacterial infection and pneumonia, 32 dysregulated proteins related to Staphylococcus aureus infection, bacterial invasion of epithelial cells, and pathogenic Escherichia coli infection were screened out. Among these 32 proteins, 13 proteins were mapped to the bovine genome. Given the close phylogenetic relationships of FMD and bovine, the protein-protein interaction networks of the above-mentioned 13 proteins were constructed by the String database. Based on the node degree analysis, 5 potential key proteins related to pneumonia-related bacterial infection in FMD were filtered out. Moreover, 85 dysregulated proteins related to the immune system process were identified given the tight connection between immune dysregulation and pneumonia pathogenesis. Additionally, 12 proteins that might function as crucial players in pneumonia-related immune response in FMD were screened out using the same experimental strategies described above. In conclusion, some vital proteins, biological processes, and pathways in pneumonia development were identified in FMD.

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Section: Screening and Ppi Network Construction Of Dysregulated Prote...mentioning

confidence: 99%

“…The increase in the population of FMD is also limited by some fatal diseases including pneumonia ( 11 – 13 ). Pneumonia can be caused by multiple pathogens including bacteria ( 14 – 16 ).…”

Section: Introductionmentioning

confidence: 99%

ITRAQ-based quantitative proteomics analysis of forest musk deer with pneumonia

Tang

Suo²,

Li³

et al. 2022

Front. Vet. Sci.

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“…is an endangered species in China and is currently listed as a class I protected species at the national level [3,4,5]. Musk is secreted by the musk gland in the groin of adult male forest musk deer, it has high economic and medicinal value [6,7]. In recent years, due to habitat destruction and other reasons, the population of wild forest musk deer has decreased sharply [4].…”

Section: Introductionmentioning

confidence: 99%

First report on molecular identification of Eimeria sp. from captive forest musk deer

Ren

Zhao

et al. 2020

Preprint

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Background: Forest musk deer (Moschus berezovskii) is a national first-level protected and endangered wild animals in China. The intestinal coccidiosis of captive forest musk deer is one of the most important diseases. However, few studies have been conducted to quantify Eimeria sp. infection and to identify its molecules. Thus, the objective of this study was to investigate the Eimeria sp. infection in the intestinal tract of forest musk deer in Sichuan and Shaanxi, China, and to identify the 18S rRNA gene fragment of Eimeria sp. , which provides scientific basic experimental data for the molecular epidemiological investigation and population genetic analysis of Eimeria sp. of captive forest musk deer in 7 regions.Methods: 328 faecal samples of forest musk deer were collected from 7 farms. The DNA of Eimeria sp. in the positive samples was extracted and used as template for nested PCR amplification. The 18S rRNA gene fragment was connected with the plasmid vector, and the products were introduced into Escherichia coli (DH5α). The culture bacterial solution was used as a PCR reaction template for identification.Results: In total, we collected 328 faecal samples from forest musk deer in Lixian (n=54), Maoxian (n=52), Ma’erkang (n=49), Dujiangyan (n=55), Hanyuan (n=41), Luding (n=36) and Weinan (n=41). 198 (60.37%) faecal samples were positive for Eimeria sp. We analyzed the 18S rRNA gene sequence of Eimeria sp, and determined 34 types with similarity of 90.5% ~ 100%. A phylogenetic tree constructed based on the 18S rRNA gene sequence of Eimeria sp., it was confirmed that Eimeria sp. parasitized in the intestinal tract of forest musk deer was closely related to Eimeria alabamensis from Bos taurus, Eimeria faurei from Ovis aries and Eimeria ahsata from Ovis aries.Conclusions: To our knowledge, this was the first molecular identification of Eimeria sp. in the intestinal tract of forest musk deer. The study suggested that Eimeria sp. parasitizes in the intestinal tract of forest musk deer, which is their carrier.

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Investigation and molecular identification of Eimeria sp. sampled from captive forest musk deer

Ren

Zhao

et al. 2021

PeerJ

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Forest musk deer (Moschus berezovskii) is an endangered, protected species in China. Intestinal coccidiosis is a significant problem for captive forest musk deer. However, there are few reports on the prevalence and molecular characteristics of Eimeria sp. in forest musk deer. We sought to investigate the prevalence of Eimeria sp. in forest musk deer in the Sichuan and Shaanxi provinces in China. We also investigated the molecular characteristics of Eimeria sp. by analyzing the 18S rRNA gene. We collected a total of 328 fecal samples from forest musk deer on seven farms throughout the Sichuan and Shaanxi provinces. We extracted this parasite’s DNA and used this as a template for nested PCR amplification. The 18S rRNA gene fragment was associated with the plasmid vector, and these products were introduced into Escherichia coli (DH5α). The cultured bacterial solution was used as a PCR reaction template for identification purposes. We collected 328 fecal samples from forest musk deer in Lixian (n = 54), Maoxian (n = 52), Ma’erkang (n = 49), Dujiangyan (n = 55), Hanyuan (n = 41), Luding (n = 36) and Weinan (n = 41). One hundred ninety-eight (60.37%) fecal samples tested positive for Eimeria sp. . In our analysis of the 18S rRNA gene we found 34 types of Eimeria sp. with a similarity of 90.5–100%. We constructed a phylogenetic tree based on the parasite’s 18S rRNA gene sequence. Our findings indicated that the Eimeria sp. that parasitized the intestinal tract of forest musk deer was closely related to Eimeria alabamensis from Bos taurus and Eimeria ahsata from Ovis aries. To the best of our knowledge, ours was the first investigation and molecular identification of Eimeria sp. sampled from captive forest musk deer in China. Our results provide epidemiological data for the monitoring and prevention of Eimeria sp. in captive forest musk deer.

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Affinity of Streptococcal G Protein to Forest Musk Deer (Moschus berezovskii) Serum Immunoglobulin G

Cited by 3 publications

References 11 publications

ITRAQ-based quantitative proteomics analysis of forest musk deer with pneumonia

ITRAQ-based quantitative proteomics analysis of forest musk deer with pneumonia

First report on molecular identification of Eimeria sp. from captive forest musk deer

Investigation and molecular identification of Eimeria sp. sampled from captive forest musk deer

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