Affinity screening of potential anti-obesity and anti-diabetic component from pomegranate peel by co-immobilization of lipase and α-amylase using carbon nanotube and hydrogel

Wang, Shuai; Liu, Shuo; Liu, Chang; Tang, Shanshan; Gu, Dongyu; Tian, Jing; Yang, Yi

doi:10.1016/j.procbio.2023.01.002

Cited by 18 publications

(4 citation statements)

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“…The amylase inhibitory effect of ellagic acid, however, remains controversial. While some studies have reported that it possesses both in silico (Bansode & Salalkar, 2016;Wang et al, 2023) and in vitro (Wang et al, 2023) amylase inhibitory activity and could potentiate hypoglycaemic action by inhibiting starch hydrolysis (Bellesia et al, 2015), other studies have reported that it possesses little to no amylase inhibitory activity Kam et al, 2012). Documented evidence has revealed the notable contribution of tannins to the α-amylase inhibitory action Ó 2023 Institute of Food, Science and Technology (IFSTTF).…”

Section: Preliminary Antioxidant Enzyme Inhibitory and Phytochemical ...mentioning

confidence: 99%

Temperature‐dependent extraction and chromatographic recovery and characterisation of ellagitannins with potent antioxidant and glycaemic control properties from ‘Wonderful’ pomegranate peel

Mashile,

Setlhodi,

Izu

et al. 2023

Int J of Food Sci Tech

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SummaryPomegranate peel contains bioactive ellagitannins including punicalagin, a major bioactive principle. However, studies have not optimally recovered and evaluated these ellagitannins for their antioxidant and glycaemic control potential. In the present study, a temperature‐dependent extraction was employed to optimally recover bioactive ellagitannins and punicalagin from pomegranate (variety ‘Wonderful’) peel. The peel was extracted with distilled water at different temperatures (25, 37, 50, 65, 78 and 95 °C) and evaluated for phenol and flavonoid contents, in vitro antioxidant and enzyme inhibitory activities. XAD16N resin was used to recover tannins from the extracts and punicalagin was purified from the tannin recovered from the 78 °C crude extract. LC–MS was used to characterise the ellagitannins profile. The dose‐dependent anti‐lipid peroxidative and glucose uptake potential of the punicalagin and its parent crude extract and tannin were measured. The extract obtained at 78 °C had the highest phenolic, flavonoid, tannin and punicalagin contents, which potentiated stronger radical scavenging, ORAC (IC50 = 9.64 vs. 15.6 and 25.6 μg/mL, respectively), anti‐linoleic acid peroxidative (IC50 = 9.97 vs. 19.9 and 27.3 μg/mL, respectively) and antiglycation (IC50 = 29.1 vs. 34.2 and 79.1 μg/mL, respectively) activities than the extract and the purified punicalagin. Similarly, the cellular anti‐lipid peroxidative and glucose modulatory effects of the recovered tannin (IC50 = 3.67 μg/mL; EC50 = 14.8 μg/mL) better than that of the extract (IC50 = 24.3 μg/mL; EC50 = 55.0 μg/mL) and the purified punicalagin (IC50 = 59.0 μg/mL; EC50 = 112 μg/mL), which may be attributed to the complementary action of the tannin's constituent compounds (punicalagin, ellagic acid, granatin A, corilagin, casuarinin, gallic acid and quercetin hexoside). The α‐amylase inhibitory data, however, suggest the purified punicalagin influences enzyme inhibitory potential of pomegranate peel. The study suggests tannin/ellagitannin purified from pomegranate peel aqueous extract obtained at 78 °C may be a promising dietary supplement for exerting glycaemic control and mitigating oxidative stress.

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Section: Preliminary Antioxidant Enzyme Inhibitory and Phytochemical ...mentioning

confidence: 99%

Temperature‐dependent extraction and chromatographic recovery and characterisation of ellagitannins with potent antioxidant and glycaemic control properties from ‘Wonderful’ pomegranate peel

Mashile,

Setlhodi,

Izu

et al. 2023

Int J of Food Sci Tech

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“…CRL's crystal structure (PDB ID: 1CRL), having a good resolution (2.06 Å), was retrieved from the Research Collaboratory for Structural Bioinformatics (RCSB) Protein Data Bank (PDB) site, https://www.rcsb.org, accessed on 17 April 2023 [19]. This enzyme is a member of the family of hydrolases (carboxylic esterases) and comprises 534 AAs, wherein AAs Ser209, His449, and Glu341 comprise the key catalytic triad essential for hydrolysis of acylglycerides [23,24].…”

Section: Structure Retrieval and Preparationmentioning

confidence: 99%

“…This SOPMA analysis can give insight into the individual AAs involved in building the secondary structure with their positions [28]. The structure of CRL was extracted from RCSB [19]. After the completion of the secondary structure analysis, this extracted structure of CRL was further subjected to various online servers to assess its 3D structure and possible stereochemical quality using different analyses such as Protein Structure Analysis (ProSA) (https://prosa.services.came.sbg.ac.at/prosa.php, accessed on 18 April 2023), PROCHECK, VERIFY-3D [20], and ERRAT (https://saves.mbi.ucla.edu/, accessed on 18 April 2023) [29].…”

Section: Secondary Structural Analysis and Homology Modeling Studies ...mentioning

confidence: 99%

“…In addition, tools such as molecular docking and molecular dynamics (MD) simulation can make predictions about enantioselectivity, potential catalytic deactivations, or compound affinity, which arise from interactions between an enzyme, substrate, and solvent, more rapidly and with less need for expensive materials [ 16 , 17 , 18 ]. The molecular process and the intermolecular interactions can both be understood using MD modeling [ 19 ]. Computational methods have been useful in the literature for elucidating the link between enzyme structure and function.…”

Section: Introductionmentioning

confidence: 99%

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Molecular Modeling Insights into Metal-Organic Frameworks (MOFs) as a Potential Matrix for Immobilization of Lipase: An In Silico Study

Patil

Kamble

Dhanavade

et al. 2023

Biology

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CRL is a highly versatile enzyme that finds extensive utility in numerous industries, which is attributed to its selectivity and catalytic efficiency, which have been impeded by the impracticality of its implementation, leading to a loss of native catalytic activity and non-reusability. Enzyme immobilization is a necessary step for enabling its reuse, and it provides methods for regulating the biocatalyst’s functional efficacy in a synthetic setting. MOFs represent a novel category of porous materials possessing distinct superlative features that make MOFs an optimal host matrix for developing enzyme-MOF composites. In this study, we employed molecular modeling approaches, for instance, molecular docking and MD simulation, to explore the interactions between CRL and a specific MOF, ZIF-8. The present study involved conducting secondary structural analysis and homology modeling of CRL, followed by docking ZIF-8 with CRL. The results of the molecular docking analysis indicate that ZIF-8 was situated within the active site pocket of CRL, where it formed hydrogen bonds with Val-81, Phe-87, Ser-91, Asp-231, Thr-132, Lue-297, Phe-296, Phe-344, Thr-347, and Ser-450. The MD simulation analysis revealed that the CRL and ZIF-8 docked complex exhibited stability over the entire simulation period, and all interactions presented in the initial docked complex were maintained throughout the simulation. The findings derived from this investigation could promote comprehension of the molecular mechanisms underlying the interaction between CRL and ZIF-8 as well as the development of immobilized CRL for diverse industrial purposes.

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Artificial antibody-antigen-directed immobilization of lipase for consecutive catalytic synthesis of ester: Benzyl acetate case study

Yang,

Guo,

Guo

et al. 2024

Bioresource Technology

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Affinity screening of potential anti-obesity and anti-diabetic component from pomegranate peel by co-immobilization of lipase and α-amylase using carbon nanotube and hydrogel

Cited by 18 publications

References 50 publications

Temperature‐dependent extraction and chromatographic recovery and characterisation of ellagitannins with potent antioxidant and glycaemic control properties from ‘Wonderful’ pomegranate peel

Temperature‐dependent extraction and chromatographic recovery and characterisation of ellagitannins with potent antioxidant and glycaemic control properties from ‘Wonderful’ pomegranate peel

Molecular Modeling Insights into Metal-Organic Frameworks (MOFs) as a Potential Matrix for Immobilization of Lipase: An In Silico Study

Artificial antibody-antigen-directed immobilization of lipase for consecutive catalytic synthesis of ester: Benzyl acetate case study

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