Spices and herbs are widely consumed, due to their cooking benefits, unique flavors, colors, aromas and medicinal importance. Unfortunately, they are liable to be heavily contaminated with toxigenic fungi with subsequent production of aflatoxins (AFs). Acute and chronic exposure to aflatoxin is considered a serious health risk problem. This study aimed to determine the level of AFs in some unpacked spices and herbs sold in local markets of Al Majmmah province. 45 unpacked spices and herbs samples including ginger, turmeric, cinnamon, cardamom, cumin, black pepper, chili pepper, cloves, laurel leaves and aniseed were collected randomly from local markets of Al Majmaah province, KSA from March to June 2017. The presence of AFs was determined by HPLC. AFs mainly, AFB1were detected in 13 samples. All results were below the limit of 10 ug/kg for total AFs. AFB1 levels were below 5ug/kg except for three turmeric samples.The levels of total AFs in all contaminated samples are in the permissible European limits. For AFB1, only three turmeric samples were beyond the European limits. Regular monitoring of unpacked spices is required.