Aflatoxin B1 Degradation by Metabolites of Phoma glomerata PG41 Isolated From Natural Substrate Colonized by Aflatoxigenic Aspergillus flavus

Щербакова, Л. А.; Statsyuk, N. V.; Mikityuk, O. D.; Nazarova, T. A.; Dzhavakhiya, V. G.

doi:10.5812/jjm.24324

Cited by 35 publications

(28 citation statements)

References 12 publications

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“…Decontamination of toxin-contained substrates is an urgent problem for the agriculture and health [1]. There are at least two possible ways to reduce aflatoxin B1 contamination: the blocking of toxin biosynthesis or the degradation of already produced toxin [2].…”

Section: Introductionmentioning

confidence: 99%

“…After fermentation, the AFB1 content in culture broth was quantified by high performance liquid chromatography (HPLC) as described earlier [2]. Stimulating or inhibiting effects on the AFB1 biosynthesis was evaluated as compared to control (incubation without tested substances).…”

Section: Introductionmentioning

confidence: 99%

“…After the chloroform evaporation, the residue was dissolved in 200 μl of methanol. The AFB1 concentration was further determined by HPLC [2].…”

Section: Introductionmentioning

confidence: 99%

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SOME NATURAL AND SYNTHETIC COMPOUNDS INHIBITING THE BIOSYNTHESIS OF AFLATOXIN B1 AND MELANIN IN Aspergillus flavus

Dzhavakhiya¹,

Voinova²,

Popletaeva³

et al. 2016

S-h. biol.

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A b s t r a c tThe control of the mycotoxin contamination of agricultural products represents a serious problem of the global food and feed industry. Aflatoxin B1 (AFB1) is one of the most dangerous mycotoxins due to its hepatotoxicity, carcinogenicity, and temperature resistance. Thus, the search for substances able to block its biosynthesis and, therefore, to prevent the toxin accumulation in food and feed is still relevant. This paper is devoted to the study of the ability of some natural and synthesized compounds to block the biosynthesis of AFB1 and/or melanin (both are secondary metabolites, which have common intermediates and common initial stages of the polyketide biosynthetic pathway) in toxigenic Aspergillus flavus. The studied compounds included lovastatin, and several commercial compounds: (aminoethyl)thiophosphonic acid, (aminomethyl)thiophosphonic acid, alafosfalin, (1-aminoethyl)phosphonic acid, and N-hydroxyputrescine. A mutant Aspergillus terreus strain 45-50 obtained earlier from the A. terreus ATCC 20542 was used as the lovastatin producer. N-hydroxyputrescine and some phosphoanalogues of amino acids were assessed by their influence on the pigmentation of fungal colonies grown on solid medium and on the specific AFB1 content in cultural broth determined after the cultivation of a fungus on liquid nutrition medium. According to the obtained results, the studied compounds were divided into three groups. To reveal changes in the colony morphology, toxigenic A. flavus strain AF11 was cultivated on agarized medium. Concentrations of tested compounds in the medium varied from 0.0001 to 0.1 % depending on their activity. To determine the effect of tested compounds on the AFB1 production, AF11 was cultivated for 170 h in a liquid Payne-Hagler medium. Solutions of the tested compounds were added to the medium up to the final concentration of 0.001-0.1 % (commercial compounds) or 0.0001 to 0.001 % (lovastatin). The efficiency of the AFB1 biosynthesis stimulation/inhibition was assessed by the comparison of its content in the cultural broth in the experimental and control (medium without additions) variants. In addition, the effect of lovastatin on the AFB1 accumulation in wheat grain contaminated with A. flavus AF11 was assessed. The performed screening allowed us to divide the studied compounds into three groups. The supplement of the nutrition medium with (aminoethyl)thiophosphonic acid, (aminomethyl)thiophosphonic acid, and alafosfalin caused a significant decrease in the AFB1 production, but did not influenced on the colony pigmentation. N-hydroxyputrescine and (1-aminoethyl)phosphonic acid were able to partially or completely block the melanin biosynthesis with the simultaneous increase in the AFB1 production. Lovastatin completely blocked both AFB1 and melanin production even at low concentrations (0.0005 %). Therefore, compounds from the first and second groups inhibit the AFB1 or melanin biosynthesis, respectively, via the blocking of stages located after the point of divergence of the correspon...

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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SOME NATURAL AND SYNTHETIC COMPOUNDS INHIBITING THE BIOSYNTHESIS OF AFLATOXIN B1 AND MELANIN IN Aspergillus flavus

Dzhavakhiya¹,

Voinova²,

Popletaeva³

et al. 2016

S-h. biol.

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“…The enzymatic nature of the detoxifying activity of СL in а such biodestructor, Phoma glomerata (strain PG41), has been confirmed [18].…”

mentioning

confidence: 87%

“…AFB1 was added to sterile samples of the high molecular weight fraction and the mixture was incubated at 27-28 С for 3 days. Residual amounts of B1 in СL or in the sample of the fraction were determined using high performance liquid chromatography [18].…”

mentioning

confidence: 99%

STUDY OF AFLATOXIN B1-DESTROYING ACTIVITY OF Gliocladium roseum AND Trichoderma viride AND THEIR АNTAGONISM TOWARD TOXIGENIC Aspergillus flavus

Щербакова¹,

Mikityuk²,

Nazarova³

et al. 2016

S-h. biol.

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A b s t r a c t Aflatoxin B1-destroying activity and antagonistic potential of Gliocladium roseum GRZ7 and Trichoderma viride TV35 strains isolated from natural substrates colonized by aflatoxigenic Aspergillus flavus were studied in vitro. Submerged cultures of G. roseum grown on liquid Czapek's medium with casein hydrolizate (Czapek-CasH) at 28 С and 200 rpm for 7 days were able to destroy 80-90 % of aflatoxin B1 (AFB1), which was added in the nutrient medium before inoculation. T. viride grown under the same conditions destroyed only 48 % of initial AFB1 during the same time of cultivation. The tested T. viride strain effectively suppressed the growth of toxigenic A. flavus strain A11 on Czapek-CasH agar. Co-cultivation of A11 with T. viride TV35 resulted in 64 % diminution of the average colony diameter of the aflatoxigenic strain. The strain GRZ7 of G. roseum was ineffective as an antagonist of A11. AFB1-destroyimg activity was detected in samples of high-molecular weight metabolites (> 5 kDa) isolated from culture liquid of G. roseum grown without AFB1. In addition, T. viride ability to degrade the mycotoxin was shown to be inducible. Obtained results were supposed to be of interest for further investigation on decontamination of feeds, which are contaminated with AFB1 or AFB1-producers.

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Rapid biodegradation of aflatoxin B1 by metabolites of Fusarium sp. WCQ3361 with broad working temperature range and excellent thermostability

Wang

Hui

et al. 2016

J Sci Food Agric

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Aflatoxin B1 Degradation by Metabolites of Phoma glomerata PG41 Isolated From Natural Substrate Colonized by Aflatoxigenic Aspergillus flavus

Cited by 35 publications

References 12 publications

SOME NATURAL AND SYNTHETIC COMPOUNDS INHIBITING THE BIOSYNTHESIS OF AFLATOXIN B1 AND MELANIN IN Aspergillus flavus

SOME NATURAL AND SYNTHETIC COMPOUNDS INHIBITING THE BIOSYNTHESIS OF AFLATOXIN B1 AND MELANIN IN Aspergillus flavus

STUDY OF AFLATOXIN B1-DESTROYING ACTIVITY OF Gliocladium roseum AND Trichoderma viride AND THEIR АNTAGONISM TOWARD TOXIGENIC Aspergillus flavus

Rapid biodegradation of aflatoxin B1 by metabolites of Fusarium sp. WCQ3361 with broad working temperature range and excellent thermostability

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