Aflatoxins in Foods and Feedstuffs. Part 3. Fluorodensitometric method for determination of aflatoxins M₁ and M₂ in presence of aflatoxins B₁, B₂, G₁ and G₂¹

Abstract

The method of aflotoxins M, and M, determination in presence of aflatoxins B,, B,, G, and G, is presented. The fluorescence properties of aflatoxin M, and M, are discribed. A simple method of aflatoxin M extraction is proposed. Results of different tests for confirmation of aflatoxins M are discussed. During control of powdered milk from three factories aflatoxins M were not detected in final products. As aflatoxins M, and M, usually accompany aflatoxins B,, B,, G, and G, as well as their other metabolites present mainly in milk and milk products it is reasonable to determine in the samples affected by mould simultaneously all six mentioned toxins [I, 3, 8, 9, 101. To achieve this simultaneously the determination of all six toxins has been carried out in order to choose the most convenient method for extraction, purification and chromatographic separation. Further more there have been examined the fluorescent properties of the aflatoxins MI and M, in solutions and on chromatograms. Material and MethodsA quantitative standard of the aflatoxin M, having a concentration of 10,14 pg/ml and moreover a standard of the aflatoxin M, having a concentration 6,o Fg/ml. These standards have been received from the Northern Marketing and Nutrition Research Division, Fermentation Laboratory Peoria, Illinois. A qualitative standard of the aflatoxin M,, from the Central Veterinary Laboratory, New Haw Weybridge Surrey, England. Echtblausalz B (fast blue salt B for chromatography) from the firm Merck (di-o-anisidine, tetrazolium chloride).