AFLP analysis and zebra disease resistance identification of 40 sisal genotypes in China

Abstract

Sisal is the most important fiber crop in tropical and subtropical areas in China and the world. Zebra disease is a serious threat to the main cultivar Agave hybrid No.11648 (H.11648) worldwide. To select germplasm materials with zebra disease resistance for breeding, the fluorescent amplified fragment length polymorphism (AFLP) technique was used to make a cluster analysis of the genetic relationships of 40 sisal genotypes grown in China, and Phytophthora nicotianae was used to inoculate the 40 genotypes to identify their resistance to zebra disease. As a result, the similarity coefficient among 40 sisal genotypes was found to be 0.44-0.83 and the 40 genotypes show different levels of disease resistance. According to the AFLP analysis, the disease resistance and chromosomal ploidy, it can be reasoned that, A. attenuata var. marginata, Dong 109, Nan ya 1 and A. attenuata are suitable for hybridization with H.11648 to breed a new disease-resistant variety.

Transcriptome Comparison Reveals Distinct Selection Patterns in Domesticated and Wild Agave Species, the Important CAM Plants

“…Furthermore, two of the three unigenes (GAHT01070676 and GAHT01099649) were subjected to purifying selection in A. sisalana (Table 3), which might be harmful for growth and development. This might also be responsible for the susceptibility to zebra disease caused by Phytophthora nicotianae in A. sisalana [36].…”

PENYAKIT ZEBRA PADA SISAL (AGAVE SPP.): TANTANGAN BAGI PENGEMBANGAN SISAL DI INDONESIA DAN MANAGEMEN PENGENDALIANNYA;Zebra Disease of Sisal: Challenge for Sisal Development in Indonesia and Its Control Management

“…Namun, saat ini penggunaaan fungisida kurang disukai karena aplikasinya yang sulit dan kurang ekonomis. Menurut Gao et al, (2012) kurangnya keberhasilan penggunaan fungisida untuk mengendalikan penyakit zebra karena lapisan lilin pada permukaan daun yang tebal menyulitkan penyerapan fungisida. Jadi untuk lokasi-lokasi pengembangan yang baru, penggunaan fungisida sebaiknya yang bersifat sistemik dan diaplikasi melalui tanah.…”

Development of a Specific Nested PCR Assay for the Detection of 16SrI Group Phytoplasmas Associated with Sisal Purple Leafroll Disease in Sisal Plants and Mealybugs

“…is the most important hard fibre crop in the world [ 1 ]. Unfortunately, worldwide, it is infected by numerous fungal pathogens, including Aspergillus welwitschiae [ 1 ], Phytophthora nicotianae [ 2 ], and Neoscytalidium dimidiatum [ 3 ], and it is infested by insects such as Dysmicoccus neobrevipes [ 4 ] and Scyphophorus acupunctatus [ 5 ]. Purple leafroll disease (SPLD) is currently the most destructive disease affecting sisal in China.…”