AFLP analysis of Agave tequilana varieties

Gil-Vega, Katia del Carmen; Díaz, Corina; Nava-Cedillo, Alejandro; Simpson, June

doi:10.1016/j.plantsci.2005.12.014

Cited by 58 publications

(37 citation statements)

References 9 publications

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“…These results represent a strong contrast with most of the reports on ISTR markers indicating that a large number of loci are detected in a single ISTR analysis [24,29,30] , particularly with those of reported for Agave fourcroydes, in which from 94-111 loci were detected [29] . Our results also represent a contrast with the 43-135 loci detected with AFLPs for eight species of Agave [31] and with the 93-262 loci founded also with AFLPs for several varieties of Agave tequilana Weber [16] , but are according to the results of Torres-Morán et al…”

Section: Resultscontrasting

confidence: 72%

“…Although it was affirmed that markers revealing a great number of loci are more efficient in revealing polymorphisms [16] the number of fragments obtained from ISTR analysis is enough to revel intrapopulation variability. Our results suggest that ISTR markers could be used in taxonomic analysis to delimitate and discriminate among species of Agavaceae.…”

Section: Resultsmentioning

confidence: 99%

“…azul, species like Agave durangensis, which support local industries of alcoholic beverages and which with just begin an industrialization process, techniques are needed to guarantee the botanical origin of plants, according to the respective origin denomination statement. Molecular characterization is an important technique, which with plant typification and identification can be made in a relative easy and fast manner [16] . Several marker systems are based on retrotransposons [17,18] , these markers are a ubiquitous and ancient particular class of transposable elements of plant genome [19] , replicating through a cycle of successive transcription, reverse transcription and integration into genome [20] .…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Taxonomic Significance of ISTR to Discriminate Species in Agavaceae

Torres-Morán¹,

Almaraz-Abarca²,

Velasco-Ramírez³

et al. 2008

American J. of Agricultural and Biological Sciences

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Section: Resultscontrasting

confidence: 72%

Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Taxonomic Significance of ISTR to Discriminate Species in Agavaceae

Torres-Morán¹,

Almaraz-Abarca²,

Velasco-Ramírez³

et al. 2008

American J. of Agricultural and Biological Sciences

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“…Although studies on Agave an- gustifolia are scarce (Vera Guzmán et al, 2009;Esqueda et al, 2011;Mejía-Franco and Arzate-Fernández, 2011;Bautista and Smit, 2012; Martinez Gutierrez et al, 2012;Cruz-García et al, 2013;Allsopp et al, 2013), the potential benefi ts of this lignocellulosic material cannot be ignored. Additional research on its properties is needed so that future alternative uses may be identifi ed and contribute to economic activity by increasing development opportunities, as has occurred with other species (Iñiguez-Covarrubias et al, 2001a;Iñiguez-Covarrubias et al, 2001b;Piven et al, 2001;Gil-Vega et al, 2006;Bessadok et al, 2008; Velazquez-Jimenez et al, 2013). Cooked Agave angustifolia bagasse is a material with numerous fi bers and is a largely underutilized waste material from the mezcal (alcoholic beverage) industry; however, these fi bers, similar to other plant fi bers, are lignocellulosic materials with technical, economic and ecological potential and therefore could be used in different industries.…”

Section: Introductionmentioning

confidence: 99%

Chemical and morphological characterization of <em>Agave angustifolia</em> bagasse fibers

et al. 2015

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Abstract:The main aim of this study was to characterize cooked bagasse fi bers from Agave angustifolia Haw. The fi bers were characterized using scanning electron microscopy, differential scanning calorimetry, thermogravimetric analysis, X-ray Diffraction and chemical analysis. The tensile strength was also tested using fi bers with a uniform length (30 mm). The fi bers were light brown in color, with a mean diameter and length of 501 μm and 144 mm, respectively. Scanning electron microscopy images revealed elliptically shaped cells with varying lumen size. Holocellulose content was approximately 82.12 %, and total lignin content was approximately 20.69 %. Due to the crystallinity and lignin content, the fi bers proved to be thermo-stable until 220 °C.The mean values of tensile strength, Young's modulus, % strain (ε), and ultimate tensile strength were determined via mechanical tests. The results are comparable to those of other common lignocellulosic fi bers, confi rming their potential use as a reinforcing element in a polymer matrix to form a new biodegradable composite. Keywords: chemical composition, crystallinity, mechanical tests, morphology, thermal analysis Resumen: El objetivo principal de este estudio fue caracterizar las fi bras de bagazo cocido de Agave angustifolia Haw. Las fi bras fueron caracterizadas a través de microscopia electrónica de barrido, calorimetría diferencial de barrido, análisis termogravimétri-cos, difracción de rayos X y análisis químico. También se realizaron pruebas de resistencia a la tracción usando fi bras de longitud constante (30 mm). Las fi bras presentaron un color marrón claro, con diámetro medio de 501 μm y longitud media de 144 mm. Las imágenes del microscopio electrónico de barrido mostraron células de forma elíptica con diferente tamaño de lumen. El contenido de holocelulosa fue alrededor de 82.12 % y el contenido total de lignina de aproximadamente 20.69 %. La fi bra resultó ser térmicamente estable hasta 220 °C debido a la cristalinidad y el contenido de lignina. El esfuerzo de tensión, el módulo de Young, el porcentaje de deformación (ε) y el esfuerzo último de tensión fueron obtenidos de las pruebas mecánicas. Los resultados son comparables a los de otras fi bras lignocelulósicas comunes, lo cual confi rma que estas fi bras tienen potencial como refuerzo en una matriz polimérica para formar un nuevo compuesto biodegradable. Palabras clave: análisis térmico, composición química, cristalinidad, morfología, pruebas mecánicas. ETHNOBOTANY R ecently, research on plant fi bers has been increasing due to the abundance of these materials and their status as renewable resources (Joseph et al., 1999;Ghali et al., 2006;Lucena et al., 2009;Ku et al., 2011;Kestur et al., 2013). Consequently, this focus has led to further research on the specifi c characterization of individual plant fi bers such as bamboo, okra, sisal, and henequen (Mishra et al., 2004; Bé-akou et al., 2008;De Rosa et al., 2010;Liu et al., 2012;Arrakhiz et al., 2013) and studies on composite materials (Mohan...

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“…A. tequilana Weber, in particular, shows a high amount of non-redundant haplotypes with seven for each rDNA gene. The presence of this genetic dynamism could be considered as a strategy of fast evolution due to the intensive cultivation and vegetative propagation of some of these species (e.g., A. tequilana Weber) (ColungaGarcíaMarín et al 1999;Gil-Vega et al 2001;Infante et al 2003;Gil-Vega et al 2006). A. tequilana Weber has been under selection stress for several decades in comparison to A. fourcroydes Lem.…”

Section: Occurrence and Significance Of Non-redundant Rdna Haplotypesmentioning

confidence: 99%

Comparative Characterization of Ribosomal DNA Regions in Different Agave Accessions with Economical Importance

2015

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Two ribosomal DNA regions (5S and 18S) were characterized in three economically important species of Agave Linnaeus, 1753 namely Agave tequilana Weber, 1902; Agave angustifolia Haworth, 1915; and Agave fourcroydes Lemaire, 1864 which are used to produce several products such as tequila, mezcal, and hard fibers. Characterization included Agave L. accessions with different ploidy levels (2n=2x=60 to 2n=6×=180) in order to relate this factor with copy number, haplotype number, expression profile, and predictable functionality of ribosomal DNA (rDNA) sequences. Only total rDNA copy number (5S and 18S) was related with ploidy level. Main differences were found in the 5S rDNA gene since it exhibited different genetic traits of Agave L. accession. In this gene, four different allelic groups (I, 105; II, 107; III, 110; and IV, 111 bp) were detected, which have probably evolved separately, thus exhibiting different expression profiles and different haplotype occurrence. Allelic groups III and IV exhibit the highest number of total and expressed copies in all Agave L. accessions. Nonredundant haplotypes were probably more functional in these allelic groups. Differences between the Agave L. accessions were more clearly observed in the most cultivated accession, A. tequilana (2n=2×=60), where the allelic group III shows non-redundant haplotypes and is transcriptionally upregulated suggesting a different evolutionary pressure on this Agave L. accession.

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AFLP analysis of Agave tequilana varieties

Cited by 58 publications

References 9 publications

Taxonomic Significance of ISTR to Discriminate Species in Agavaceae

Taxonomic Significance of ISTR to Discriminate Species in Agavaceae

Chemical and morphological characterization of <em>Agave angustifolia</em> bagasse fibers

Comparative Characterization of Ribosomal DNA Regions in Different Agave Accessions with Economical Importance

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