AFLP Analysis of Intraspecific Variation Between <i>Monilinia laxa</i> Isolates from Different Hosts

Gril, T.; Celar, F.; Munda, A.; Javornik, Branka; Jakse, Jernej

doi:10.1094/pdis-92-12-1616

Cited by 28 publications

(20 citation statements)

References 27 publications

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“…According to this fact and the considerable variability within the populations revealed with MP-PCR, it might be concluded that there was no host specialisation in the investigated Monilinia population in Hungary. Gril et al (2008) carried out an analysis with AFLP marker system which shows that Monilinia isolates were not grouped according to geographic origin.…”

Section: Resultsmentioning

confidence: 99%

“…Gell et al (2007) were the first in Europe to study M. laxa populations from several orchards in Spain, using RAPD markers revealing the highest genetic diversity within subpopulations. Gril et al (2008) analyzed the genetic diversity and relationships of Monilinia laxa isolates obtained from different host plants with amplified fragment length polymorphism (AFLP) marker system. Their results showed host restricted specialization of isolates, with M. laxa isolates from apple clearly distinguished.…”

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confidence: 99%

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Characterization of M. laxa and M. fructigena isolates from Hungary with MP-PCR

Szôdi

Komjáti

Turóczi

2012

Hort. Sci. (Prague)

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Monilinia laxa (Monilia laxa), Monilinia fructicola (Monilia fructicola) and Monilinia fructigena (Monilia fructigena) are the causal agents of brown rot on pome and stone fruits in Hungary. Forty-five isolates collected from different hosts, different years in several orchards were used for characterization of the M. laxa and M. fructigena population in Hungary. The isolates were identified on species level based on morphological and molecular biological methods; out of these 24 were M. laxa, 20 were M. fructigena and 1 was M. fructicola. Populations of the three Monilinia species were studied with microsatellite primers and the degree of genetic diversity within the species was measured. The population structure analysis revealed that genetic diversity within M. laxa subpopulations was H S = 0.1599, while within M. fructigena subpopulations was H S = 0.2551. The total genetic diversity was H T = 0.3846, while genetic diversity between M. laxa and M. fructigena subpopulations was D ST = 0.1771. No clustering relationship was observed among isolates by the different years or hosts.

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Section: Resultsmentioning

confidence: 99%

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confidence: 99%

Characterization of M. laxa and M. fructigena isolates from Hungary with MP-PCR

Szôdi

Komjáti

Turóczi

2012

Hort. Sci. (Prague)

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“…

No indication of strict host associations in a widespread mycoparasite: Grapevine powdery mildew (Erysiphe necator) is attacked by phylogenetically diverse Ampelomyces strains in the field. However, in some groups of fungal plant pathogens genetic differentiation did not always correlate with host range patterns (11,14,46,47). Some strains have already been developed as commercial biocontrol agents (BCAs) of Erysiphe necator and other powdery mildew species infecting important crops.

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confidence: 99%

No Indication of Strict Host Associations in a Widespread Mycoparasite: Grapevine Powdery Mildew (Erysiphe necator) Is Attacked by Phylogenetically Distant Ampelomyces Strains in the Field

Pintye¹,

Bereczky²,

Kovács³

et al. 2012

Phytopathology®

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Pycnidial fungi belonging to the genus Ampelomyces are common intracellular mycoparasites of powdery mildews worldwide. Some strains have already been developed as commercial biocontrol agents (BCAs) of Erysiphe necator and other powdery mildew species infecting important crops. One of the basic, and still debated, questions concerning the tritrophic relationships between host plants, powdery mildew fungi, and Ampelomyces mycoparasites is whether Ampelomyces strains isolated from certain species of the Erysiphales are narrowly specialized to their original mycohosts or are generalist mycoparasites of many powdery mildew fungi. This is also important for the use of Ampelomyces strains as BCAs. To understand this relationship, the nuclear ribosomal DNA internal transcribed spacer (ITS) and partial actin gene (act1) sequences of 55 Ampelomyces strains from E. necator were analyzed together with those of 47 strains isolated from other powdery mildew species. These phylogenetic analyses distinguished five major clades and strains from E. necator that were present in all but one clade. This work was supplemented with the selection of nine inter-simple sequence repeat (ISSR) markers for strain-specific identification of Ampelomyces mycoparasites to monitor the environmental fate of strains applied as BCAs. The genetic distances among strains calculated based on ISSR patterns have also highlighted the genetic diversity of Ampelomyces mycoparasites naturally occurring in grapevine powdery mildew. Overall, this work showed that Ampelomyces strains isolated from E. necator are genetically diverse and there is no indication of strict mycohost associations in these strains. However, these results cannot rule out a certain degree of quantitative association between at least some of the Ampelomyces lineages identified in this work and their original mycohosts.

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“…Several studies have already reported variability among isolates of the same Monilinia species, such as that performed by Gril et al (2008), who used the AFLP technique and verified variability among 67 isolates of M. laxa. Villarino et al (2012) found genetic differences among isolates of M. fructicola from French and Spanish orchards.…”

Section: Isolatesmentioning

confidence: 94%

Identification and variability of Monilinia spp. isolates from peach

Casarin

Casa-Coila

Rossetto

et al. 2017

Agraria

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The brown rot caused by the fungus Monilinia spp. is the main disease of peach (Prunus persica) that occurs from flowering to post-harvest fruit. The objective of this study was to identify the species that causes brown rot in Rio Grande do Sul state using molecular method based on specific primers and study the morphological variability of Monilinia isolates using classic characterization methods. The isolates were collected in the main producing regions of peaches and determination of species was performed by using specific primers to the genus Monilinia. The culture media BDA  , peach juice and Pagnocca were used for the morphological characterization of isolates. Molecular study showed that all isolates from Rio Grande do Sul are the species M. fructicola. In the morphological characterization, such as mycelial diameter, color, margin, shape and elevation of the colonies and dry mass of mycelium, there are variability in the analyzed characteristics of the isolates in the three-culture media used. In the comparing the culture media, the higher dry matter values and diameter of colony isolates were obtained in Pagnocca and peach juice means. Key words: phenotypic characterization; brown rot; Prunus persica Identificação e variabilidade de isolados de Monilinia spp. de pessegueiro RESUMOA podridão parda causada pelo fungo Monilinia spp. é a principal doença que ocorre desde a floração até a pós-colheita de frutos de pessegueiro (Prunus persica) no Brasil. O objetivo deste trabalho foi identificar a espécie causadora da podridão parda no Rio Grande do Sul usando método molecular baseado em primers específicos e estudar a variabilidade morfológica de isolados de Monilinia através dos métodos clássicos de caracterização. Os isolados foram coletados nas principais regiões produtoras de pêssegos e a determinação da espécie foi realizada através da utilização de primers específicos para o gênero Monilinia. Os meios de cultura BDA  , Suco de pêssego e Pagnocca foram utilizados para a caracterização morfológica dos isolados. Pelo estudo molecular verificou-se que todos os isolados do Rio Grande do Sul são da espécie M. fructicola. Na caracterização morfológica, como diâmetro micelial, coloração, margem, forma e elevação das colônias e massa seca de micélio, existem variabilidade nas características analisadas dos isolados nos três meios de cultura utilizados. Na comparação entre os meios de cultura, os maiores valores de massa seca e diâmetro de colônia dos isolados foram obtidos nos meios Pagnocca e suco de pêssego.Palavras-chave: caracterização fenotípica; podridão parda; Prunus persica

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AFLP Analysis of Intraspecific Variation Between Monilinia laxa Isolates from Different Hosts

Cited by 28 publications

References 27 publications

Characterization of M. laxa and M. fructigena isolates from Hungary with MP-PCR

Characterization of M. laxa and M. fructigena isolates from Hungary with MP-PCR

No Indication of Strict Host Associations in a Widespread Mycoparasite: Grapevine Powdery Mildew (Erysiphe necator) Is Attacked by Phylogenetically Distant Ampelomyces Strains in the Field

Identification and variability of Monilinia spp. isolates from peach

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