Characterization of M. laxa and M. fructigena isolates from Hungary with MP-PCR

Abstract

Monilinia laxa (Monilia laxa), Monilinia fructicola (Monilia fructicola) and Monilinia fructigena (Monilia fructigena) are the causal agents of brown rot on pome and stone fruits in Hungary. Forty-five isolates collected from different hosts, different years in several orchards were used for characterization of the M. laxa and M. fructigena population in Hungary. The isolates were identified on species level based on morphological and molecular biological methods; out of these 24 were M. laxa, 20 were M. fructigena and 1 was M. fructicola. Populations of the three Monilinia species were studied with microsatellite primers and the degree of genetic diversity within the species was measured. The population structure analysis revealed that genetic diversity within M. laxa subpopulations was H S = 0.1599, while within M. fructigena subpopulations was H S = 0.2551. The total genetic diversity was H T = 0.3846, while genetic diversity between M. laxa and M. fructigena subpopulations was D ST = 0.1771. No clustering relationship was observed among isolates by the different years or hosts.

Susceptibility of Serbian plum cultivars to indigenous bacterial and Monilinia laxa isolates

“…The ability of indigenous M. laxa isolates to cause typical symptoms on artificially inoculated mature fruits of the Rodna and Požegača cultivars agrees with field observations of plum susceptibility to M. laxa in Serbian plum orchards (Hrustić et al 2015;Glišić et al 2016). The obtained data are also in accordance with the results of studies from other plum-producing areas across the Europe, where M. laxa was commonly detected in plum orchards affected by brown rot (Gell et al 2007;Sződi et al 2012;Poniatowska et al 2013). The observed differences in the virulence of M. laxa isolates on fruits of the Rodna and Požegača cultivars is possibly attributable to the existence of different plum genotypes, causing higher resistance of the Rodna cultivar.…”