AFLP analysis of phylogenetic relationships among myrmecophytic species of Macaranga (Euphorbiaceae) and their allies

Bänfer, Gudrun; Fiala, Brigitte; Weising, Kurt

doi:10.1007/s00606-004-0219-4

Cited by 40 publications

(50 citation statements)

References 36 publications

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“…The number of clones investigated in the present paper is also given Section Species No. of clones investigated generated because target loci are distributed all across the genome (Bänfer et al 2004). AFLP genotyping is often chosen due to the high number of polymorphic markers generated in a single PCR experiment and the high reproducibility (Gemeinholzer and Bachmann 2005;Bonin et al 2007).…”

Section: Introductionmentioning

confidence: 99%

Genetic structure of the genus Lemna L. (Lemnaceae) as revealed by amplified fragment length polymorphism

Bog¹,

Baumbach²,

Schween

et al. 2010

Planta

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Duckweeds (Lemnaceae) are extremely reduced in morphology, which made their taxonomy a challenge for a long time. The amplified fragment length polymorphism (AFLP) marker technique was applied to solve this problem. 84 clones of the genus Lemna were investigated representing all 13 accepted Lemna species. By neighbour-joining (NJ) analysis, 10 out of these 13 species were clearly recognized: L. minor, L. obscura, L. turionifera, L. japonica, L. disperma, L. aequinoctialis, L. perpusilla, L. trisulca, L. tenera, and L. minuta. However, L. valdiviana and L. yungensis could be distinguished neither by NJ cluster analysis nor by structure analysis. Moreover, the 16 analysed clones of L. gibba were assembled into four genetically differentiated groups. Only one of these groups, which includes the standard clones 7107 (G1) and 7741 (G3), represents obviously the "true" L. gibba. At least four of the clones investigated, so far considered as L. gibba (clones 8655a, 9481, 9436b, and Tra05-L), represent evidently close relatives to L. turionifera but do not form turions under any of the conditions tested. Another group of clones (6745, 6751, and 7922) corresponds to putative hybrids and may be identical with L. parodiana, a species not accepted until now because of the difficulties of delineation on morphology alone. In conclusion, AFLP analysis offers a solid base for the identification of Lemna clones, which is particularly important in view of Lemnaceae application in biomonitoring.

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Section: Introductionmentioning

confidence: 99%

Genetic structure of the genus Lemna L. (Lemnaceae) as revealed by amplified fragment length polymorphism

Bog¹,

Baumbach²,

Schween

et al. 2010

Planta

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“…Thus, the AFLP marker technique will provide additional information on genome-wide differentiation patterns between species. This technique has been successfully used to analyze differentiation at the species or genus level (Sharma et al 1996;Koopman et al 2001;Banfer et al 2004;Brouat et al 2004;Gailing and von Wuehlisch 2004). We expected to obtain novel insights into the evolution of dipterocarps in Southeast Asia by this application of a combination of different molecular marker techniques with traditional taxonomic assessments.…”

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confidence: 99%

Genetic variation at AFLPs for the Dipterocarpaceae and its relation to molecular phylogenies and taxonomic subdivisions

et al. 2006

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Genetic differentiation was investigated among 54 Indonesian species of Dipterocarpaceae, a dominant tree family in Asian tropical rainforests, using amplified fragment length polymorphism markers. The tree developed from the resultant unweighted pair group method using arithmetic averages clearly separated all investigated dipterocarps into two major groups that corresponded to tribe Dipterocarpeae and tribe Shoreae, respectively. These results are in accordance with the topology of molecular phylogenetic trees derived from PCR-restriction fragment length polymorphism analysis of chloroplast DNA and generally support the traditional taxonomic assessments. The possibility of interspecific hybridization is also discussed.

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“…In earlier studies, we successfully reconstructed phylogenetic trees of Macaranga myrmecophytes with the help of nuclear ribosomal ITS sequences (Blattner et al 2001) and amplified fragment length polymorphisms (Bänfer et al 2004). Our previous attempts at using chloroplast DNA (cpDNA) haplotypes for generating species-specific phylogeographies were however hampered by the frequent occurrence of shared haplotypes among closely related Macaranga species (Vogel et al 2003).…”

Section: Introductionmentioning

confidence: 99%

Comparative chloroplast DNA phylogeography of two tropical pioneer trees, Macaranga gigantea and Macaranga pearsonii (Euphorbiaceae)

Guicking

Fiala

Blattner

et al. 2011

Tree Genetics & Genomes

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Macaranga (Euphorbiaceae) has received much ecological and evolutionary research attention as a genus that includes some of the most conspicuous pioneer trees of Southeast Asian tropical rainforests and because of its manifold associations with ants, including about 30 species that are obligate ant-plants (myrmecophytes). We used sequence data from three chloroplast DNA loci (ccmp5, ccmp6, atpB-rbcL) to assess phylogeographical patterns in species of Macaranga, section Pruinosae, sampled from various regions of Borneo and the Malay Peninsula. Fortynine chloroplast DNA haplotypes (HT) were identified among 768 specimens from five species, Macaranga gigantea (N= 329; 23 HT), Macaranga pearsonii (N=347; 21 HT), Macaranga puberula (N=24; 4 HT), Macaranga hosei (N= 48; 6 HT), and Macaranga pruinosa (N=20; 5 HT). Fortyone haplotypes were species-specific, whereas eight haplotypes were shared by two, three, or four species and occupied internal positions in a parsimony network. Population genetic parameters based on haplotype frequencies proved to be in a similar range in the non-myrmecophytic M. gigantea and in the ant-associated M. pearsonii, which have overlapping distributions in northern and eastern Borneo. A comparison of G ST and N ST values revealed a strong phylogeographic structure in both species, whereas colonization pathways suggested by the network topology were different. Both species exhibited similar levels of haplotypic diversity and moderate to high levels of population differentiation. There were no obvious indications for an influence of the symbiotic ant partners on the population structure of their host plants.

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AFLP analysis of phylogenetic relationships among myrmecophytic species of Macaranga (Euphorbiaceae) and their allies

Cited by 40 publications

References 36 publications

Genetic structure of the genus Lemna L. (Lemnaceae) as revealed by amplified fragment length polymorphism

Genetic structure of the genus Lemna L. (Lemnaceae) as revealed by amplified fragment length polymorphism

Genetic variation at AFLPs for the Dipterocarpaceae and its relation to molecular phylogenies and taxonomic subdivisions

Comparative chloroplast DNA phylogeography of two tropical pioneer trees, Macaranga gigantea and Macaranga pearsonii (Euphorbiaceae)

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