Ag and Mn-doped mesoporous bioactive glass nanoparticles incorporated into the chitosan/gelatin coatings deposited on PEEK/bioactive glass layers for favorable osteogenic differentiation and antibacterial activity

Abstract

<p>Bioactive and antibacterial coatings were developed and characterized in this study. Chitosan/gelatin/Ag-Mn doped mesoporous bioactive glass nanoparticles (Ag-Mn MBGNs) composite coatings (of 5-6 µm thickness) were deposited using electrophoretic deposition...</p>

Chitosan/gelatin‐based bioactive and antibacterial coatings deposited via electrophoretic deposition

“…Moreover, the infiltration of the top layer may be useful for the optimum release of incorporated metallic ions, which will lead to the therapeutic effect. A similar mechanism was also reported by Nawaz et al 35 The thickness of the multi‐structured coatings was in the range of 70 μm (Figure 2(c)). The SEM image at the cross‐section shown in Figure 2(c) indicates that the deposits were uniform in thickness.…”

“…Thus, the surface roughness and WCA of the multi‐structured coatings are suitable for upregulating the protein intake and attachment of the bone‐forming cells. Moreover, gelatin can further enhance the biocompatibility of the coatings because of its arginine–glycine–aspartic acid (RGD) and related sequences 35,36 …”

“…The intensity of amide‐I and amide‐II strongly decreases with the increase in the immersion period, which indicates the degradation of chitosan and gelatin. The degradation of chitosan/gelatin is expected in the physiological environment and is useful for the favorable osteogenic differentiation 35 . The phosphate band was noticed due to the stretching at 562, 605, and 1014 cm ‐1.…”

Ag–Sr doped mesoporous bioactive glass nanoparticles loaded chitosan/gelatin coating for orthopedic implants

“…Furthermore, the presence of Sr will support the bioactivity of the coatings and the presence of Ag will provide a potent antibacterial effect. 19 The bioactivity of the deposited coatings may decrease due to the presence of Ag in MBGNs. However, the presence of Sr was intended to mitigate the possible toxic effect of the Ag.…”

“…The results have shown that the 5 mol.% addition of MnO in the MBGNs supported osteogenic differentiation and enhanced the upregulation of genes encoding for extracellular matrix proteins. 17 Recently, Nawaz et al 19 showed that the incorporation of Mn and Ag in MBGNs promotes the antibacterial and osteogenic response. Aiah et al 20 doped MBGNs with silver ions and found that the Ag-MBGNs were bioactive and antibacterial against gram-positive and gram-negative bacteria.…”