Agaricus brasiliensis-derived β-glucans exert immunoenhancing effects via a dectin-1-dependent pathway

Yamanaka, Daisuke; Tada, Rui; Adachi, Yoshiyuki; Ishibashi, Ken; Motoi, Masuro; Iwakura, Yoichiro; Ohno, Naohito

doi:10.1016/j.intimp.2012.07.017

Cited by 37 publications

(15 citation statements)

References 36 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…This receptor also provides signals for the production of reactive oxygen intermediates (ROI) and for the release of cytokines such as TNF-α, IL-2, IL-6, IL-10, and IL-23 [35–37]. In addition, Ohno’s group have demonstrated that immunoenhancing effects of A. brasiliensis- derived β-glucans are mediated by the dectin-1-signaling pathway [38]. However, as reported by this same research group, the amount of β-glucan in ATF can be not enough to efficiently activate βGR [39].…”

Section: Discussionmentioning

confidence: 99%

Agaricus brasiliensis polysaccharides stimulate human monocytes to capture Candida albicans, express toll-like receptors 2 and 4, and produce pro-inflammatory cytokines

Martins

Soares

Domeneghini

et al. 2017

J Venom Anim Toxins Incl Trop Dis

View full text Add to dashboard Cite

Background Agaricus brasiliensis is a medicinal mushroom with immunomodulatory and antitumor activities attributed to the β-glucans presented in the polysaccharide fraction of its fruiting body. Since β-glucans enhance cellular immunoresponsiveness, in this study we aimed to evaluate the effect of an acid-treated polysaccharide-rich fraction (ATF) of A. brasiliensis on the ability of human monocytes to adhere/phagocyte C. albicans yeast cells, their expression of pattern recognition receptors and their ability to produce cytokines.MethodsAdhesion/phagocytosis of FITC-labeled C. albicans was evaluated by flow cytometry. Cells were incubated with specific fluorochrome-labeled antibodies for TLR2 and 4, βGR and MR and also evaluated by flow cytometry. Monocytes were cultured with ATF, and culture supernatants were collected for analysis of in vitro cytokine production by ELISA (TNF-α, IL-1β, IL-12 and IL-10).ResultsATF significantly increased the adherence/phagocytosis of C. albicans by monocytes and this was associated with enhanced expression of TLR2 and TLR4, while no effect was observed on βGR or MR. Moreover, expression of TLR4 and TLR2 was associated with higher levels of in vitro production of TNF-α and IL-1, respectively. Production of IL-10 was also increased by ATF treatment, but we found no association between its production and the expression of Toll-like receptors.ConclusionOur results provided us with evidence that A. brasiliensis polysaccharides affect human monocytes probably through the modulation of Toll-like receptors.

show abstract

Section: Discussionmentioning

confidence: 99%

Agaricus brasiliensis polysaccharides stimulate human monocytes to capture Candida albicans, express toll-like receptors 2 and 4, and produce pro-inflammatory cytokines

Martins

Soares

Domeneghini

et al. 2017

J Venom Anim Toxins Incl Trop Dis

View full text Add to dashboard Cite

show abstract

“…The sulfation has gained special interest because the β-glucans increase their solubility avoiding granuloma formation. In recent years, several authors have reported the activity of these polysaccharides in healing wounds and burns as well as the antimutagenic, anticarcinogenic, and immunomodulatory activities (Angeli et al 2009;Sui et al 2010;Yamanaka et al 2012). …”

Section: Fungimentioning

confidence: 99%

Polysaccharide Production by Submerged Fermentation

Montoya

Vargas

2015

Polysaccharides

View full text Add to dashboard Cite

“…The mechanism that polysaccharides activate innate immune system is assumed to conduct through signal pathway via Toll-like receptors. Dentin-1-deficient mice would cause dramatically reduction in cytokine production in bone marrow-derived dendritic cells (Yamanaka et al 2012). Macrophage is a pivotal factor in the process, while polysaccharides act as nonself substances in the body to activate the receptors in the macrophage surface.…”

Section: Immunomodulationmentioning

confidence: 99%