Agarose Gel Electrophoresis of Cerebrospinal Fluid Proteins and Analysis of the Pherogram Profiles by Analog Computer

Siegert, Martín J.; Siemes, H.

doi:10.1515/cclm.1977.15.1-12.635

Cited by 7 publications

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References 9 publications

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“…Die Auswertung der Phoretogramme wurde mit einem von Siegert und Siemes [40] modifizierten Analogrechner vorgenommen. Hierbei wurden die registrierten Phoretogramme auf dem Analogrechner mit Hilfe von elf elektronischen Generatoren, mit denen Gauss-Kurven variabler H6he und Breite in beliebigen Horizontalpositionen erzeugt werden k6nnen, entsprechend den verschiedenen Proteinfraktionen nachgebildet.…”

Section: Agarosegel-elektrophorese Und Analogrechnerauswertungunclassified

“…Fiir die Beurteilung des Verlaufes der Erkrankung ist die Konzentration der Plasmaproteine, insbesondere die des Albumins ein beachtenswerter Parameter.Zur Bestimmung der Plasmaproteine wurden die von Siegert et al [40,41] ausgearbeitete quantitative Agarosegel-Elektrophorese und die Analogrechneranalyse der Pherogrammprofile fiir den quantitativen Mikronachweis modifiziert. For the analyses carried out in the course of hyperbilirubinemia, 6 gl of serum were necessary in each case, of which 50-80 gg were used for electrophoresis.…”

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“…Zur Bestimmung der Plasmaproteine wurden die von Siegert et al [40,41] ausgearbeitete quantitative Agarosegel-Elektrophorese und die Analogrechneranalyse der Pherogrammprofile fiir den quantitativen Mikronachweis modifiziert.…”

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Die Serumproteine der wachsenden Gunn-Ratte

1981

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Microprocesses of quantitative agarose gel electrophoresis and biuret total protein evaluation were used for the quantitative estimation of the serum proteins of homozygous and heterozygous Gunn rats. For the analyses carried out in the course of hyperbilirubinemia, 6 microliter of serum were necessary in each case, of which 50-80 microgram were used for electrophoresis. The quantitative evaluation of the phoretogram curves plotted at two wavelengths was carried out with the aid of an analog computer. Eleven fractions were quantitatively covered by the process. Age-related concentration shifts were observed for a number of fractions, especially for albumin, transferrin, and IgG. The results obtained from a large number of animals in longitudinal and cross-sectional examination corroborate various reports found in the relevant literature.

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Section: Agarosegel-elektrophorese Und Analogrechnerauswertungunclassified

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Die Serumproteine der wachsenden Gunn-Ratte

1981

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“…The amido black stained pherograms were evaluated by an analog Computer program (7). Transferrin was identifled by a reference Standard (8).…”

Section: Introductionmentioning

confidence: 99%

Pathobiochemical Alterations in Experimental Chronic and Acute Trypanosomal Infection in Mice

Takeya

Reinwald²,

Risse³

1987

Clinical Chemistry and Laboratory Medicine

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During an experimental chronic infection of inbred mice with Trypanosoma congolense several physiological parameters become altered. Splenomegaly followed later by hepatomegaly are predominant. Lactate dehydrogenase and aminotransferase activities of the plasma are elevated, the number of erythrocytes and thrombocytes decreases, whereas monocytic cells are detected in higher concentrations. -Globulins and transferrin become elevated. Some of the pathobiochemical alterations depend directly on the parasitaemia and are reversed to normal values after chemotherapy with diminazene aceturate (Berenil®). The curative effect of this drug depends largely on when it is administered.In acute T. congolense infections, leading to the death of the animals in 3-4 days, pathobiochemical alterations are found only shortly before the exitus.

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“…The total protein concentration was determined by the Biuret method (Biochemia-Test Combination, Boehringer, Mannheim). Quantitative agarose gel electrophoresis was used for protein analysis (23).…”

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OCCURRENCE OF OLIGOCLONAL GAMMAGLOBULIN IN THE CSF OF CHILDREN WITH PROLONGED and CHRONIC CNS‐INFECTIONS

1981

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CSF-proteins of 1770 children and adolescents with different neurological diseases and of 75 controls were examined by zone electrophoresis in agarose gel electrophoresis and by immunofixation electrophoresis. The quantitative evaluation of the phoretograms by an analog computer revealed oligoclonal changes of the gamma-globulin profile in 53 patients with subacute or chronic CNS-infections and in 5 children with a medulloblastoma. Seventeen of 37 children with congenital infections had 1--5 oligoclonal gamma-fractions consisting of IgG. Five to seven oligoclonal IgG fractions were detected in each of 16 children with SSPE. All 6 adolescents with multiple sclerosis had 2--5 oligoclonal IgG fractions. One to five oligoclonal gamma-bands occurred transiently in the CSF of 4 children with prolonged meningoencephalitis caused by different viruses, in 3 children with a prolonged non-bacterial meningitis of probable viral origin, in 2 infants with prolonged bacterial meningitis after corticosteroid therapy, and in 1 child with prolonged bacterial meningitis during cytostatic therapy. Four to six oligoclonal gamma-subfractions were found at different times during progressive viral encephalitis that developed during maintenance therapy of acute lymphoblastic leukaemia, and in 2 patients with chronic meningoencephalitis of unknown origin. Since oligoclonal gamma-globulin was detected almost invariably in the CSF of patients with prolonged or chronic neurologic infections, this finding implies persistence of antigens in the CNS and pathologic invasion of lymphocytes with selective proliferation of antigen-stimulated clones.

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Agarose Gel Electrophoresis of Cerebrospinal Fluid Proteins and Analysis of the Pherogram Profiles by Analog Computer

Cited by 7 publications

References 9 publications

Die Serumproteine der wachsenden Gunn-Ratte

Die Serumproteine der wachsenden Gunn-Ratte

Pathobiochemical Alterations in Experimental Chronic and Acute Trypanosomal Infection in Mice

OCCURRENCE OF OLIGOCLONAL GAMMAGLOBULIN IN THE CSF OF CHILDREN WITH PROLONGED and CHRONIC CNS‐INFECTIONS

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