Age and Diet Affect Gene Expression Profile in Canine Skeletal Muscle

Middelbos, Ingmar S.; Vester, Brittany M.; Karr-Lilienthal, Lisa K.; Schook, Lawrence B.; Swanson, Kelly S.

doi:10.1371/journal.pone.0004481

Cited by 18 publications

(19 citation statements)

References 78 publications

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“…It has also been reported that WFDC2 is involved in inflammatory responses and host defense, and its activity is increased in chronically inflamed lungs with cystic fibrosis [12]. Increased expression of genes related to inflammation and immune response was also observed in other tissues from these dogs [6], [7], [8]. In our previous experiment [4], senior dogs fed APB had a greater concentration of blood cholesterol than those fed PPB diet.…”

Section: Discussionsupporting

confidence: 68%

Age and Diet Affect Gene Expression Profiles in Canine Liver Tissue

et al. 2010

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BackgroundThe liver plays a central role in nutrient and xenobiotic metabolism, but its functionality declines with age. Senior dogs suffer from many of the chronic hepatic diseases as elderly humans, with age-related alterations in liver function influenced by diet. However, a large-scale molecular analysis of the liver tissue as affected by age and diet has not been reported in dogs.Methodology/Principal FindingsLiver tissue samples were collected from six senior (12-year old) and six young adult (1-year old) female beagles fed an animal protein-based diet (APB) or a plant protein-based diet (PPB) for 12 months. Total RNA in the liver tissue was extracted and hybridized to Affymetrix GeneChip® Canine Genome Arrays. Using a 2.0-fold cutoff and false discovery rate <0.10, our results indicated that expression of 234 genes was altered by age, while 137 genes were differentially expressed by diet. Based on functional classification, genes affected by age and/or diet were involved in cellular development, nutrient metabolism, and signal transduction. In general, gene expression suggested that senior dogs had an increased risk of the progression of liver disease and dysfunction, as observed in aged humans and rodents. In particular for aged liver, genes related to inflammation, oxidative stress, and glycolysis were up-regulated, whereas genes related to regeneration, xenobiotic metabolism, and cholesterol trafficking were down-regulated. Diet-associated changes in gene expression were more common in young adult dogs (33 genes) as compared to senior dogs (3 genes).ConclusionOur results provide molecular insight pertaining to the aged canine liver and its predisposition to disease and abnormalities. Therefore, our data may aid in future research pertaining to age-associated alterations in hepatic function or identification of potential targets for nutritional management as a means to decrease incidence of age-dependent liver dysfunction.

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Section: Discussionsupporting

confidence: 68%

Age and Diet Affect Gene Expression Profiles in Canine Liver Tissue

et al. 2010

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“…Hybridization reactions were performed with reagents f in accordance with the manufacturer' s instructions and as previously described. 17 After hybridization, microchips were washed and stained with a streptavidin-conjugated phycoerthyrin dye enhanced with biotinylated goat anti-streptavidin antibody via a wash and stain station g and associated software. Images of microchips were acquired by use of a scanning system.…”

Section: Methodsmentioning

confidence: 99%

Gene expression in intestinal mucosal biopsy specimens obtained from dogs with chronic enteropathy

Wilke¹,

Nettleton²,

Wymore³

et al. 2012

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Objective—To characterize mucosal gene expression in dogs with chronic enteropathy (CE). Animals—18 dogs with CE and 6 healthy control dogs. Procedures—Small intestinal mucosal biopsy specimens were endoscopically obtained from dogs. Disease severity in dogs with CE was determined via inflammatory bowel index scores and histologic grading of biopsy specimens. Total RNA was extracted from biopsy specimens and microchip array analysis (approx 43,000 probe sets) and quantitative reverse transcriptase PCR assays were performed. Results—1,875 genes were differentially expressed between dogs with CE and healthy control dogs; 1,582 (85%) genes were downregulated in dogs with CE, including neurotensin, fatty acid–binding protein 6, fatty acid synthase, aldehyde dehydrogenase 1 family member B1, metallothionein, and claudin 8, whereas few genes were upregulated in dogs with CE, including genes encoding products involved in extracellular matrix degradation (matrix metallopeptidases 1, 3, and 13), inflammation (tumor necrosis factor, interleukin-8, peroxisome proliferator–activated receptor γ, and S100 calcium-binding protein G), iron transport (solute carrier family 40 member 1), and immunity (CD96 and carcinoembryonic antigen–related cell adhesion molecule [CEACAM] 18). Dogs with CE and protein-losing enteropathy had the greatest number of differentially expressed genes. Results of quantitative reverse transcriptase PCR assay for select genes were similar to those for microchip array analysis. Conclusions and Clinical Relevance—Expression of genes encoding products regulating mucosal inflammation was altered in dogs with CE and varied with disease severity. Impact for Human Medicine—Molecular pathogenesis of CE in dogs may be similar to that in humans with inflammatory bowel disease.

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“…To identify the molecular mechanisms underlying sarcopenia, several groups have performed whole-genome expression analysis of skeletal muscle during aging at the transcriptional level in rodents (Lee et al 1999;Pattison et al 2003), monkeys (Kayo et al 2001), dogs (Middelbos et al 2009) and humans (Bortoli et al 2003;Dennis et al 2008;Giresi et al 2005;Jozsi et al 2000;Melov et al 2007;Roth et al 2002;Welle et al 2000Welle et al , 2003Welle et al , 2004Zahn et al 2006). Large numbers of genes, ranging from tens to hundreds (typical) to thousands, have been shown to undergo age-associated alterations in transcription levels.…”

Section: Gene Expression Microarray Studiesmentioning

confidence: 99%

Molecular genetic studies of gene identification for sarcopenia

et al. 2011

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Sarcopenia, which is characterized by a progressive decrease of skeletal muscle mass and function with aging, is closely related to several common diseases (such as cardiovascular and airway diseases) and functional impairment/disability. Strong genetic determination has been reported for muscle mass and muscle strength, two most commonly recognized and studied risk phenotypes for sarcopenia, with heritability ranging from 30 to 85% for muscle strength and 45-90% for muscle mass. Sarcopenia has been the subject of increasing genetic research over the past decade. This review is designed to comprehensively summarize the most important and representative molecular genetic studies designed to identify genetic factors associated with sarcopenia. We have methodically reviewed whole-genome linkage studies in humans, quantitative trait loci mapping in animal models, candidate gene association studies, newly reported genome-wide association studies, DNA microarrays and microRNA studies of sarcopenia or related skeletal muscle phenotypes. The major results of each study are tabulated for easy comparison and reference. The findings of representative studies are discussed with respect to their influence on our present understanding of the genetics of sarcopenia. This is a comprehensive review of molecular genetic studies of gene identification for sarcopenia, and an overarching theme for this review is that the currently accumulating results are tentative and occasionally inconsistent and should be interpreted with caution pending further investigation. Consequently, this overview should enhance recognition of the need to validate/replicate the genetic variants underlying sarcopenia in large human cohorts and animal. We believe that further progress in understanding the genetic etiology of sarcopenia will provide valuable insights into important fundamental biological mechanisms underlying muscle physiology that will ultimately lead to improved ability to recognize individuals at risk for developing sarcopenia and our ability to treat this debilitating condition.

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Age and Diet Affect Gene Expression Profile in Canine Skeletal Muscle

Cited by 18 publications

References 78 publications

Age and Diet Affect Gene Expression Profiles in Canine Liver Tissue

Age and Diet Affect Gene Expression Profiles in Canine Liver Tissue

Gene expression in intestinal mucosal biopsy specimens obtained from dogs with chronic enteropathy

Molecular genetic studies of gene identification for sarcopenia

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