Age and Ebola viral load correlate with mortality and survival time in 288 Ebola virus disease patients

Jin, Li; Duan, Huijuan; Chen, Haoyang; Ji, Yingjie; Zhang, Xin; Yao, Rong; Xu, Zhe; Sun, Lijian; Zhang, Jiyuan; Li, Liming; Jin, Bo; Zhang, Jian; Du, Nan; Su, Haibin; Teng, Guangju; Yue, Yuan; Qin, Enqiang; Jia, Hongjun; Shu, Wang; Guo, Tao; Wang, Ye; Mu, Jinsong; Yan, Tao; Li, Zhiwei; Dong, Zihui; Nie, Weimin; Jiang, Tianjun; Li, Chen; Gao, Xudong; Ji, Dong; Zhuang, Yijie; Li, Lei; Wang, Lifu; Li, Wengang; Duan, Xiaojiang; Lu, Yinying; Sun, Zhiqiang; Kanu, Alex; Koroma, Sheku M.; Zhao, Min; Jun-sheng, JI; Wang, Fusheng

doi:10.1016/j.ijid.2015.10.021

Cited by 64 publications

(57 citation statements)

References 13 publications

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“…Clinical and laboratory factors at presentation that were associated with a fatal outcome included fever, weakness, dizziness, diarrhea, and elevated levels of blood urea nitrogen, aspartate aminotransferase, and creatinine. Age of >45 years and a plasma EBOV load of >10 8 genomes/mL were also associated with fatal outcomes, findings that were subsequently confirmed in other cohorts [31][32][33]. Our clinical study showed that the West African variant of EBOV causes predominately a gastrointestinal illness and focused attention on this aspect for control of disease spread, rather than on bleeding manifestations that predominated in prior outbreaks.…”

Section: Contributions Of Kgh To the Scientific Understanding Of The supporting

confidence: 73%

An Outbreak of Ebola Virus Disease in the Lassa Fever Zone

Goba¹,

Khan²,

Fonnie³

et al. 2016

J Infect Dis.

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Section: Contributions Of Kgh To the Scientific Understanding Of The supporting

confidence: 73%

An Outbreak of Ebola Virus Disease in the Lassa Fever Zone

Goba¹,

Khan²,

Fonnie³

et al. 2016

J Infect Dis.

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“…Patients with EVD have viral loads, peaking above 10 5 copies/mL at the time of presentation [26]. Three studies in Sierra Leone reported a mean EBOV load at admission of 10 6 copies/mL (for 606 patients), 4.89 log copies/mL (for 632 patients), and 6.68 log copies/mL (for 288 patients) [27,28,29]. During the SUDV outbreak in Uganda during 2001, the average peak viremia level in cases with a fatal outcome was 3.4 × 10 9 RNA copies/mL, while in nonfatal cases it was 4.3 × 10 7 RNA copies/mL [26].…”

Section: Discussionmentioning

confidence: 99%

Development, Evaluation, and Integration of a Quantitative Reverse-Transcription Polymerase Chain Reaction Diagnostic Test for Ebola Virus on a Molecular Diagnostics Platform

Cnops¹,

Eede

Pettitt

et al. 2016

J Infect Dis.

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Background. The 2013-2016 Ebola epidemic in West Africa resulted in accelerated development of rapid diagnostic tests for emergency outbreak preparedness. We describe the development and evaluation of the Idylla™ prototype Ebola virus test, a fully automated sample-to-result molecular diagnostic test for rapid detection of Zaire ebolavirus (EBOV) and Sudan ebolavirus (SUDV).Methods. The Idylla™ prototype Ebola virus test can simultaneously detect EBOV and SUDV in 200 µL of whole blood. The sample is directly added to a disposable cartridge containing all reagents for sample preparation, RNA extraction, and amplification by reverse-transcription polymerase chain reaction analysis. The performance was evaluated with a variety of sample types, including synthetic constructs and whole blood samples from healthy volunteers spiked with viral RNA, inactivated virus, and infectious virus.Results. The 95% limits of detection for EBOV and SUDV were 465 plaque-forming units (PFU)/mL (1010 copies/mL) and 324 PFU/mL (8204 copies/mL), respectively. In silico and in vitro analyses demonstrated 100% correct reactivity for EBOV and SUDV and no cross-reactivity with relevant pathogens. The diagnostic sensitivity was 97.4% (for EBOV) and 91.7% (for SUDV), the specificity was 100%, and the diagnostic accuracy was 95.9%.Conclusions. The Idylla™ prototype Ebola virus test is a fast, safe, easy-to-use, and near-patient test that meets the performance criteria to detect EBOV in patients with suspected Ebola.

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“…7 RNA copies/ml blood at the time of diagnosis (typically seen as C T values of Ͻ25) (4,12,(33)(34)(35)(36). Recent evidence from a longitudinal study of four EVD survivors suggests that C T values greater than ϳ35 in the setting of convalescence are not associated with infectious virus (37); however, a C T value of Յ40 is typically used as the cutoff to designate a positive sample (37).…”

Section: Real-time Rt-pcrmentioning

confidence: 99%

Diagnosis of Ebola Virus Disease: Past, Present, and Future

2016

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SUMMARYLaboratory diagnosis of Ebola virus disease plays a critical role in outbreak response efforts; however, establishing safe and expeditious testing strategies for this high-biosafety-level pathogen in resource-poor environments remains extremely challenging. Since the discovery of Ebola virus in 1976 via traditional viral culture techniques and electron microscopy, diagnostic methodologies have trended toward faster, more accurate molecular assays. Importantly, technological advances have been paired with increasing efforts to support decentralized diagnostic testing capacity that can be deployed at or near the point of patient care. The unprecedented scope of the 2014-2015 West Africa Ebola epidemic spurred tremendous innovation in this arena, and a variety of new diagnostic platforms that have the potential both to immediately improve ongoing surveillance efforts in West Africa and to transform future outbreak responses have reached the field. In this review, we describe the evolution of Ebola virus disease diagnostic testing and efforts to deploy field diagnostic laboratories in prior outbreaks. We then explore the diagnostic challenges pervading the 2014-2015 epidemic and provide a comprehensive examination of novel diagnostic tests that are likely to address some of these challenges moving forward.

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Age and Ebola viral load correlate with mortality and survival time in 288 Ebola virus disease patients

Abstract: Patients with a high viral load, of older age, and with diarrhea had a higher mortality and shorter survival time.

Cited by 64 publications

References 13 publications

An Outbreak of Ebola Virus Disease in the Lassa Fever Zone

An Outbreak of Ebola Virus Disease in the Lassa Fever Zone

Development, Evaluation, and Integration of a Quantitative Reverse-Transcription Polymerase Chain Reaction Diagnostic Test for Ebola Virus on a Molecular Diagnostics Platform

Diagnosis of Ebola Virus Disease: Past, Present, and Future

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