Age‐associated changes to pathogen‐associated molecular pattern‐induced inflammatory mediator production in dogs

Deitschel, Sarah J; Kerl, Marie E.; Chang, Chee‐Hoon; DeClue, Amy E.

doi:10.1111/j.1476-4431.2010.00565.x

Cited by 40 publications

(34 citation statements)

References 57 publications

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“…According to previous reports, IL-10 exerts a diverse host defense response by steering the inflammatory response in different directions (Ouyang et al 2011). Increases in IL-10 production after LPS injection and quercetin treatment have been reported in dogs and rats (Deitschel et al 2010;Milenkovic et al 2010). The increase of IL-10 might be the response of the body to LPS stimulation; however, the underlying mechanism still needs to be elucidated.…”

Section: Discussionmentioning

confidence: 92%

Novel quercetin-3-O-glucoside eicosapentaenoic acid ester ameliorates inflammation and hyperlipidemia

et al. 2015

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Quercetin, a major flavonol, present abundantly in apples and onions, is widely studied for ameliorating metabolic syndrome abnormalities. However, quercetin is mainly present in plant food in the form of quercetin glycosides and has been reported for poor gastrointestinal absorption. The present study was designed with the purpose of imparting a lipophilic property to quercetin-3-O-glucoside (QG) by its acylation with eicosapentaenoic acid (EPA) and to study the influence of eicosapentaenoic acid ester of quercetin-3-O-glucoside (QE) on hyperlipidemia and inflammation in vivo and in vitro. QE was more effective in reducing the production of tumor necrosis factor-alpha (TNF-α), prostaglandin 2 (PGE2), cyclo-oxygenase (COX)-2 levels and nuclear expression of nuclear factor-kappa B (NF-кB) compared to the parent compounds (QG and EPA) and commercial drugs, after lipopolysaccharides (LPS) induced inflammation in THP-1 derived macrophages. Serum high-density lipoprotein (HDL)-cholesterol was significantly higher and hepatic total cholesterol concentration was lower in the rats fed high-fat diet supplemented with QE, compared to the high-fat diet with inflammation (HFL). The serum concentrations of C-reactive protein (CRP), interleukin (IL)-6, and interferon-gamma (IFN-γ) were significantly lower in QE treatment group than HFL group. EPA conjugated flavonol, QE, had significant anti-inflammatory and hypolipidemic properties and may be effective for the treatment of obesity-related disorders.

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Section: Discussionmentioning

confidence: 92%

Novel quercetin-3-O-glucoside eicosapentaenoic acid ester ameliorates inflammation and hyperlipidemia

et al. 2015

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“…Plasma TNF-α, IL-6 and IL-10 concentrations were measured at baseline, 3 hr (TNF-α), 6 hr, 12 hr, and 24 hr using sandwich ELISA based on commercially available matched canine antibody pairs (Duoset ELISA development kit, R&D Systems, Minneapolis, MN, U.S.A.) [9]. Assays were performed in duplicate according to the manufacturer's instructions.…”

Section: Methodsmentioning

confidence: 99%

Pathophysiologic and Immunologic Changes in a Canine Endotoxemia over a Period of 24 Hours

Kim

Park

2012

The Journal of Veterinary Medical Science

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ABSTRACT. In this study, the pathophysiologic and immunologic parameters from a 24-hr of canine endotoxemia model by lipopolysaccharide (LPS) infusion were evaluated. For that, twelve healthy beagles received a continuous 24-hr IV infusion of low dose LPS (10 µg/kg/h, from Escherichia coli serotype O111:B4) dissolved in saline. Complete blood counts and serum biochemical analysis as well as histopathologic examination were performed to assess pathophysiologic changes such as neutrophil migration and organ injury. To evaluate immunologic parameters, the concentrations of plasma tumor necrosis factor (TNF)-α, interleukin (IL)-6 and IL-10 were determined, and neutrophil activation was also evaluated based on cell surface expression of CD11b using flow cytometry analysis. As results, systemic signs of endotoxemia including fever, vomiting, and hemorrhagic diarrhea were observed within short time after LPS infusion. Severe leukopenia and increased fluorescent intensity of CD11b on neutrophils were observed at 3 hr while percent positive of CD11b was the maximum at 12 hr during the experiment. Plasma alanine aminotransferase (ALT), aspartate aminotransferase (AST), alkaline phosphatase (ALP), lactate dehydrogenase (LDH) and creatinine phosphokinase (CPK) concentrations increased markedly, and organ damage was confirmed on histopathologic examination. Plasma TNF-α peaked at 3 hr and decreased rapidly, while the concentrations of IL-6 and IL-10 increased gradually until 6 hr and decreased thereafter. Using this canine endotoxemia model, we were able to determine the kinetics of pathophysiologic and immunologic parameters over a period of 24 hr. This study will enhance our understanding of their mechanisms underlying canine sepsis.

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“…18,20,22 Briefly, murine fibroblasts (L929) were cultured on 96-well plates for 12 h, and then the samples were added. After a 20 h incubation with minimum essential medium (Invitrogen, Carlsbad, CA) plus horse serum and actinomycin D (Sigma-Aldrich), a 3-[4,5-dimethylthiazol-2-yl]-2,5,-di-phenyl tetrazolium bromide (Sigma-Aldrich) colorimetric assay was used to quantify the number of live cells per well.…”

Section: Tumor Necrosis Factormentioning

confidence: 99%

“…[18][19][20] Lipopolysaccharide (100 ng/ml) from Escherichia coli 0127:B8 (Sigma-Aldrich, St. Louis, MO), LTA (1000 ng/ml) from Streptococcus faecalis (Sigma-Aldrich), and PG (1,000 ng/ml) from Staphylococcus aureus (Sigma-Aldrich), or control [phosphate-buffered saline (PBS)] were added to the wells as described previously. 18,20 The wells were mixed thoroughly, and the plates were incubated for 24 h at 37 °C in 5% CO 2 . After incubation, the plates were centrifuged (300 g × 7 min, 4 °C) and the supernatant was collected and frozen at -80 °C until batch analyses.…”

Section: Whole Blood Culturementioning

confidence: 99%

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Upregulation of Proinflammatory Cytokine Production in Response to Bacterial Pathogen-Associated Molecular Patterns in Dogs with Diabetes Mellitus Undergoing Insulin Therapy

DeClue

Nickell

Chang

et al. 2012

J Diabetes Sci Technol

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Background: Metabolic alterations associated with diabetes mellitus alter innate immunity. Dogs often develop infectious or inflammatory complications related to diabetes mellitus, yet little is known about the effects of diabetes mellitus on the immune system in this species. Methods: Prospective evaluation in dogs with poorly regulated spontaneous type 1 diabetes mellitus (T1DM). In vitro leukocyte cytokine response to lipopolysaccharide (LPS), lipoteichoic acid (LTA), and peptidoglycan (PG) was compared between dogs with T1DM and healthy dogs. Additionally, the effect of acute in vitro glucose exposure on leukocyte tumor necrosis factor (TNF) production from healthy dogs was measured. Results: Leukocytes from dogs with T1DM had significantly greater TNF production after LTA and PG stimulation compared with leukocytes from healthy dogs. Leukocyte interleukin (IL)-6 production was greater after stimulation with LPS, LTA, PG, and phosphate-buffered saline in the T1DM group. No such difference was noted when evaluating IL-10 production between groups regardless of stimulant. Dogs with T1DM had significantly greater IL-6 to IL-10 production ratios than healthy dogs. Acute exposure to dextrose did not augment cytokine production from healthy canine leukocytes. Conclusions: Dogs with T1DM have altered innate immunity characterized by upregulation of proinflammatory cytokine production without a concurrent change in anti-inflammatory cytokine production. This may be one explanation for the common infectious and inflammatory complications associated with T1DM in dogs.

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Age‐associated changes to pathogen‐associated molecular pattern‐induced inflammatory mediator production in dogs

Abstract: PAMPs from gram-positive and gram-negative bacteria stimulate TNF, IL-6, and IL-10 production from canine whole blood. The inflammatory mediator response to PAMPs from gram-negative bacteria alters with age and may be one factor contributing to mortality in geriatric dogs with sepsis.

Cited by 40 publications

References 57 publications

Novel quercetin-3-O-glucoside eicosapentaenoic acid ester ameliorates inflammation and hyperlipidemia

Novel quercetin-3-O-glucoside eicosapentaenoic acid ester ameliorates inflammation and hyperlipidemia

Pathophysiologic and Immunologic Changes in a Canine Endotoxemia over a Period of 24 Hours

Upregulation of Proinflammatory Cytokine Production in Response to Bacterial Pathogen-Associated Molecular Patterns in Dogs with Diabetes Mellitus Undergoing Insulin Therapy

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