Age-dependence of urinary normal and modified nucleosides in childhood as determined by reversed-phase high-performance liquid chromatography

Development and validation of urinary nucleosides and creatinine assay by capillary electrophoresis with solid phase extraction

“…Therefore, it appeared reasonable and interesting to undertake measurements of urinary nucleosides in order to estimate total RNA turnover. The turnover of nucleic acids increases when cell proliferation takes place: in physiological processes, like growth and pregnancy [3] and in pathological ones, like inflammation, haemopathies and malignant diseases [4,5]. Urinary profiles of modified and normal nucleosides have been used in clinical practice as markers of leukemia [6], breast cancer [7,8], thyroid cancer [9], uterine cervical cancer [10], liver cancer [11] and rheumatoid arthritis process [12].…”

Analysis of urinary nucleosides as potential cancer markers determined using LC–MS technique

“…The most popular analytical column is C18 [6,[15][16][17][18][19][57][58][59], however HILIC columns are becoming more popular due to the polar character of compounds like nucleosides [55,60]. Besides, there is also a trend to resemble conditions like UHPLC using routine HPLC system in order to reduce eddy-diffusion and increase flow rates with controlled back pressures.…”

“…Their abnormal levels in urine are related to increased RNA turnover and higher methyltransferase activity, both of which are caused by http pathophysiological status, such as cancer. This process is not, however, dependent on age among adults [17]. Therefore, according to numerous studies, urinary nucleosides have been proposed as possible cancer markers in colorectal [18][19][20], breast [21][22][23][24][25][26], bladder [27][28][29], lymphoma [30,31], thyroid [32] and cervical cancer [33].…”

Detection of 1,N2-propano-2′-deoxyguanosine in human urine by stable isotope dilution UHPLC–MS/MS analysis

“…Various methods have been developed for the detection of urinary nucleosides, including electrochemical method [18], immunoassays [19,20], capillary electrophoresis (CE) and high performance liquid chromatography (HPLC) [17,[21][22][23][24], matrixassisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF-MS) [25,26], liquid chromatography tandem mass spectrometry (LC-MS) [27][28][29][30][31][32]. In comparison to other methods, the LC-MS based methodologies take the advantages of high sensitivity and direct analysis with structural information from MS measurements [33].…”