Age-Dependent Changes in the Inflammatory Nociceptive Behavior of Mice

King-Himmelreich, Tanya S.; Möser, Christine V.; Wolters, Miriam; Olbrich, Katrin; Geißlinger, Gerd; Niederberger, Ellen

doi:10.3390/ijms161126041

Cited by 7 publications

(7 citation statements)

References 38 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Others have reported a similar absence of thermal hyperalgesia in LBP models 35 . The absence of thermal sensitivity may be related to a loss of TRPV1 expression reported as early as one year in mouse spinal cords 103 .…”

Section: Discussionmentioning

confidence: 99%

Aging of mouse intervertebral disc and association with back pain

Vincent

Mohanty

Pinelli

et al. 2019

Bone

View full text Add to dashboard Cite

With the increased burden of low back pain (LBP) in our globally aging population there is a need to develop preclinical models of LBP that capture clinically relevant features of physiological aging, degeneration, and disability. Here we assess the validity of using a mouse model system for age-related LBP by characterizing aging mice for features of intervertebral disc (IVD) degeneration, molecular markers of peripheral sensitization, and behavioral signs of pain. Compared to three-month-old and one-year-old mice, two-year-old mice show features typical of IVD degeneration including loss of disc height, bulging, innervation and vascularization in the caudal lumbar IVDs. Aging is also associated with the loss of whole-body bone mineral density in both male and female mice, but not associated with percent lean mass or body fat. Additionally, two-year-old mice have an accumulation of TRPA1 channels and sodium channels Na v 1.8 and Na v 1.9 in the L4 and L5 lumbar dorsal root ganglia consistent with changes in nociceptive signaling. Lastly, the effect of age, sex, and weight on mobility, axial stretching and radiating pain measures was assessed in male and female mice ranging from two months to two years in a general linear model. The model revealed that regardless of sex or weight, increased age was a predictor of greater reluctance to perform axial stretching and sensitivity to cold, but not heat in mice.

show abstract

Section: Discussionmentioning

confidence: 99%

Aging of mouse intervertebral disc and association with back pain

Vincent

Mohanty

Pinelli

et al. 2019

Bone

View full text Add to dashboard Cite

show abstract

“…Animals were bred and maintained following National Health Service guidelines [22]. In behavioral experiments, 40 CD1 females between 6 and 8 weeks of age were employed, as at this age pain sensitivity is higher than in older mice [23] and thus the efficacy of analgesic treatment is more evident. Animals were divided into four categories, with 10 animals in each group: (a) vehicle injection (PBS) without PBM, (b) capsaicin injection without PBM, (c) capsaicin injection with PBM at WL 1, (d) capsaicin injection with PBM at WL 2.…”

Section: Murine Model Of Nociceptionmentioning

confidence: 99%

Analgesic effect of Photobiomodulation Therapy: An in vitro and in vivo study

Zupin

Ottaviani

Rupel

et al. 2019

Journal of Biophotonics

View full text Add to dashboard Cite

Laser therapy, also known as Photobiomodulation (PBM) is indicated to reduce pain associated with different pathologies and applied using protocols that vary in wavelength, irradiance and fluence. Its mechanisms of action are still unclear and possibly able to directly impact on pain transmission, reducing nociceptor response. In our study, we examined the effect of two specific laser wavelengths, 800 and 970 nm, extensively applied in the clinical context and known to exert important analgesic effects. Our results point to mitochondria as the primary target of laser light in isolated dorsal root ganglion (DRG) neurons, reducing adenosine triphosphate content and increasing reactive oxygen species levels. Specifically, the 800 nm laser wavelength induced mitochondrial dysregulation, that is, increased superoxide generation and mitochondrial membrane potential. When DRG neurons were firstly illuminated by the different laser protocols and then stimulated with the natural transient receptor potential cation channel subfamily V member 1 (TRPV1) ligand capsaicin, only the 970 nm wavelength reduced the calcium response, in both amplitude and frequency. Consistent results were obtained in vivo in mice, by subcutaneous injection of capsaicin. Our findings demonstrate that the effect of PBM depends on the wavelength used, with 800 nm light mainly acting on mitochondrial metabolism and 970 nm light on nociceptive signal transmission.

show abstract

“…Both of these well-characterized behaviors are easy to measure and can reveal information about age-dependent changes in central and peripheral nociceptive modulation. Previous studies have revealed conflicting changes in spontaneous formalin behavior in both mice and rats, but no study has yet investigated if age affects mechanical sensitivity following formalin injection (Gagliese and Melzack, 2000; King-Himmelreich et al, 2015). …”

Section: Introductionmentioning

confidence: 99%

Central amygdala activation of extracellular signal-regulated kinase 1 and age-dependent changes in inflammatory pain sensitivity in mice

Sadler

Gartland

Cavanaugh

et al. 2017

Neurobiology of Aging

View full text Add to dashboard Cite

Aging populations are more sensitive to noxious stimuli as a result of altered somatosensory systems. In these experiments, we examined pain-like behaviors in young, middle-aged, and old mice during peripheral inflammation to determine if the same sensitivity exists in pre-clinical animal models. Immediately following injury, middle-aged and old mice exhibited more spontaneous pain-like behaviors than young mice, matching pain prevalence in clinical populations. Middle-aged and old mice also developed persistent mechanical hypersensitivity in the injured paw. Furthermore, old mice developed mechanical hypersensitivity in the non-injured paw suggesting age-dependent changes in central nociceptive systems. To address this end, pain-related protein expression was examined in the central nucleus of the amygdala (CeA), a limbic brain region that modulates somatic pain. Following injury, increased phosphorylation of extracellular-signal regulated kinase 1 (ERK1), a protein with known nociceptive functions, was observed in the right CeA of old mice and not middle-aged or young animals. These findings suggest that age-dependent changes in supraspinal nociceptive systems may account for increased pain-like behaviors in aging populations.

show abstract

Age-Dependent Changes in the Inflammatory Nociceptive Behavior of Mice

Cited by 7 publications

References 38 publications

Aging of mouse intervertebral disc and association with back pain

Aging of mouse intervertebral disc and association with back pain

Analgesic effect of Photobiomodulation Therapy: An in vitro and in vivo study

Central amygdala activation of extracellular signal-regulated kinase 1 and age-dependent changes in inflammatory pain sensitivity in mice

Contact Info

Product

Resources

About