Age-Dependent Decline in Erythrocyte Acetylcholinesterase Activity: Correlation With Oxidative Stress

Jha, Rashmi; Rizvi, Syed Ibrahim

doi:10.5507/bp.2009.032

Cited by 42 publications

(25 citation statements)

References 29 publications

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“…It should also be noted that this discovery corroborates with Jha and Rizvi's (2009) studies that observed correlation between the antioxidant capability and the AChE activity.…”

Section: Resultssupporting

confidence: 88%

Effect of the Cymbopogon citratus Infusion on the Activity of Acetylcholinesterase Enzyme and on the Redox Profile in Farmers’ Erythrocytes

Mori¹,

Horn²,

Oliveira³

et al. 2017

JAS

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Agrochemicals were more prominent in 1960, marked due to the agricultural modernization process. As a result of this widespread use for food production, there was also an increase in cases of intoxication caused by these agents which made it necessary to search for alternative therapies for agricultural workers. Thus, considering that phytochemical characterization revealed the presence of antioxidants in Cymbopogon citratus extract, the objective of this study was to evaluate the effect of this plant infusion on the enzyme acetylcholinesterase activity (AChE) and on the redox response in farmers' erythrocytes. These erytrocytes were processed and subjected to treatment with the Cymbopogon citratus infusion (5, 10, 25 and 50 g/L). In these samples the following were determined: the AChE enzyme activity, the levels of thiobarbituric acid reactive substances (TBARS), protein carbonyls (CPs) and reduced glutathione (GSH). In general, it was discovered that the inhibition of AChE activity is negative regarding to the increase of protein carbonyl levels and positive regarding the GSH levels. In addition, Cymbopogon citratus infusions could not even reverse this inhibition or the high levels of TBARS and CPs. On the other hand, levels of GSH were increased by infusions demonstrating the increased antioxidant activity in rural workers' erythrocytes.

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“…It should also be noted that this discovery corroborates with Jha and Rizvi's (2009) studies that observed correlation between the antioxidant capability and the AChE activity.…”

Section: Resultssupporting

confidence: 88%

Effect of the Cymbopogon citratus Infusion on the Activity of Acetylcholinesterase Enzyme and on the Redox Profile in Farmers’ Erythrocytes

Mori¹,

Horn²,

Oliveira³

et al. 2017

JAS

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“…30 The determination of AChE activity in the erythrocyte membrane has been used as a free radical-mediated oxidative stress parameter during aging in humans. 31 Our results clearly show that the activity of AChE in the erythrocyte is significantly decreased in dense (senescent) erythrocyte (fraction 2) of young (4-month-old) rats in comparison to fraction 1 and un-fractionated erythrocytes of young rats. The pattern of decrease in AChE activity in young and senescent erythrocytes was the same in 24-month-old rats, but the level of enzyme activity was much lower in old rats compared to 4-month-old rats.…”

supporting

confidence: 54%

Markers of Oxidative Stress in Senescent Erythrocytes Obtained from Young and Old Age Rats

Kumar

Rizvi

2014

Rejuvenation Research

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The role of oxidative stress during aging is well documented. Evidence is available linking animal life span to the development of oxidative stress. Up to a certain limit of oxidative stress, cells function to counteract the oxidant effects and to restore redox balance by resetting critical homeostatic parameters. Red blood cells (RBCs) offer a very good model to study cellular senescence. In vivo aging of red blood cells is associated with increased cellular density, which corresponds to increased cell age. The present study aims to investigate agedependent oxidative stress in RBC subpopulations obtained after Percoll density gradient centrifugation from young and old rats. We observe an increase in plasma membrane redox system (PMRS) activity ( p < 0.001) and lipid peroxidation ( p < 0.001) between less dense and senescent RBCs in both young and old rats. Our findings provide evidence of a higher level of oxidative stress in senescent erythrocytes, with the effect being more pronounced in old (24-month-old) rats compared to young (4-month-old) rats. The present findings emphasize the role of oxidative stress not only in organismal aging but also in cell senescence.

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“…AChE has an essential role in acetylcholine-mediated neurotransmission. It is present in the cholinergic synapses in the central nervous system as well as in neuromuscular synapses where it rapidly hydrolyzes acetylcholine (Igisu et al , 1994; Jha & Rizvi, 2009). The determination of AChE activity in blood is a great consideration in the diagnosis of poisonings caused by reversible and irreversible inhibitors of this enzyme including pesticides (Bukowska & Hutnika, 2006).…”

Section: Introductionmentioning

confidence: 99%

Oxidative stress biomarkers and acetylcholinesterase activity in human erythrocytes exposed to clomazone (in vitro)

Santi¹,

Menezes²,

Duarte³

et al. 2011

Interdisciplinary Toxicology

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The aim of this study was to investigate the effect of clomazone herbicide on oxidative stress biomarkers and acetylcholinesterase activity in human erythrocytes in in vitro conditions. The activity of catalase (CAT), superoxide dismutase (SOD) and acetylcholinesterase (AChE), as well as the levels of thiobarbituric acid reactive substances (TBARS) and reduced glutathione (GSH) were measured in human erythrocytes exposed (in vitro) to clomazone at varying concentrations in the range of 0, 100, 250 and 500 µg/L for 1 h at 37 °C.TBARS levels were significantly higher in erythrocytes incubated with clomazone at 100, 250 and 500 µg/L. However, erythrocyte CAT and AChE activities were decreased at all concentrations tested. SOD activity was increased only at 100 µg/L of clomazone. GSH levels did not change with clomazone exposure. These results clearly showed clomazone to induce oxidative stress and AChE inhibition in human erythrocytes (in vitro). We, thus, suggest a possible role of ROS on toxicity mechanism induced by clomazone in humans.

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Age-Dependent Decline in Erythrocyte Acetylcholinesterase Activity: Correlation With Oxidative Stress

Cited by 42 publications

References 29 publications

Effect of the Cymbopogon citratus Infusion on the Activity of Acetylcholinesterase Enzyme and on the Redox Profile in Farmers’ Erythrocytes

Effect of the Cymbopogon citratus Infusion on the Activity of Acetylcholinesterase Enzyme and on the Redox Profile in Farmers’ Erythrocytes

Markers of Oxidative Stress in Senescent Erythrocytes Obtained from Young and Old Age Rats

Oxidative stress biomarkers and acetylcholinesterase activity in human erythrocytes exposed to clomazone (in vitro)

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