Age-Dependent Effect of Long-Term Microwave Radiation on Postnatal Neurogenesis in Rats: Morphological and Behavioral Study

Abstract

Processes of adult neurogenesis can be influenced by environmental factors. Here, we investigated the effect of microwave radiation (MWR) on proliferation and cell dying in the rat rostral migratory stream (RMS) -a migration route for the neuroblasts of the subventricular zone. Adult and juvenile (two weeks old) rats were exposed to a pulsed-wave MWR at the frequency of 2.45 GHz for 1 or 3 h daily during 3 weeks. Adult rats were divided into two groups: without survival and with two weeks survival after irradiation. Juvenile rats survived till adulthood, when were tested in the light/dark test. Proliferating cells in the RMS were labeled by Ki-67; dying cells were visualized by Fluoro-Jade C histochemistry. In both groups of rats irradiated as adults we have observed significant decrease of the number of dividing cells within the RMS. Exposure of juvenile rats to MWR induced only slight decrease in proliferation, however, it strikingly affected cell death even two months following irradiation. In addition, these rats displayed locomotor hyperactivity and decreased risk assessment in adulthood. Our results suggest that the long-lasting influence of radiation is manifested by affected cell survival and changes in animals´ behavior.

2.4 GHz Electromagnetic Field Influences the Response of the Circadian Oscillator in the Colorectal Cancer Cell Line DLD1 to miR-34a-Mediated Regulation

“…Comprehensive meta-analysis confirmed that RF-EMF predominantly influence the growth of human and faster-growing cells (45% and 47% of the included studies, respectively), and the observed effects are cell type- and dose-dependent under in vitro [ 130 ] and in vivo [ 131 , 132 , 133 ] conditions. However, in HCT-116 and DLD1 cells, RF-EMF from 900–2100 MHz applied up to 4 h did not induce changes in proliferation, as assessed by the MTT test [ 134 ]. These results are in line with our study, as we also did not observe a significant effect of RF-EMF on cell proliferation when miR-34a was not administered.…”