Age-dependent expression profiles of two adaptogenic systems and thermotolerance in Drosophila melanogaster

Shilova, Victoria; Zatsepina, Olga G.; Zakluta, Alexey S.; Карпов, Д. С.; Chuvakova, Lyubov N.; Гарбуз, Д. Г.; Evgen’ev, Michael B.

doi:10.1007/s12192-020-01074-4

Cited by 15 publications

(11 citation statements)

References 67 publications

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“…Heat shock genes encode proteins with different molecular weights and functions generally involved in proteostasis under normal and stressful conditions in all eukaryotic organisms [ 89 ]. There are scattered data suggesting some cross-talk between the genes participating in methionine metabolism and hsps [ 19 , 90 , 91 ].…”

Section: Discussionmentioning

confidence: 99%

“…The deletion of CBS gene in flies using CRISPR/Cas9 system . Construction of the system for CBS gene deletion by the integration of the mCherry expressing reporter construct has been previously described in detail [ 19 ]. To make sure there are no off-target sites of integrated reporter construct in transgenic flies, Southern blot analysis was performed.…”

Section: Methodsmentioning

confidence: 99%

“…To make sure there are no off-target sites of integrated reporter construct in transgenic flies, Southern blot analysis was performed. Isolation of genomic DNA from adult flies and Southern blot analysis to detect new insertions in CBS gene was performed according to Evgen'ev et al [ 23 ]; Shilova et al [ 19 ].…”

Section: Methodsmentioning

confidence: 99%

“…It was shown that patients with genetic defects in the TSP are characterized by high levels of homocysteine, low levels of GSH and increased incidence of age-related pathologies [ 4 , 12 ]. There is growing evidence in favour of cross-talk between H 2 S and NO levels playing an important role in cardioprotection [ 2 , 18 ], as well as the interaction between TSP and another ancient stress-response system comprising heat shock proteins (Hsps) induced by many stimuli in various organisms [ 19 ].…”

Section: Introductionmentioning

confidence: 99%

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Genome-wide transcriptional effects of deletions of sulphur metabolism genes in Drosophila melanogaster

et al. 2020

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In recent years, the gasotransmitter hydrogen sulphide (H 2 S), produced by the transsulphuration pathway, has been recognized as a biological mediator playing an important role under normal conditions and in various pathologies in both eukaryotes and prokaryotes. The transsulphuration pathway (TSP) includes the conversion of homocysteine to cysteine following the breakdown of methionine. In Drosophila melanogaster and other eukaryotes, H 2 S is produced by cystathionine β-synthase (CBS), cystathionine γ-lyase (CSE), and 3-mercaptopyruvate sulphurtransferase (MST). In the experiments performed in this study, we were able to explore the CRISPR/Cas9 technique to obtain single and double deletions in homozygotes of these three major genes responsible for H 2 S production in Drosophila melanogaster . In most cases, the deletion of one studied gene does not result in the compensatory induction of two other genes responsible for H 2 S production. Transcriptomic studies demonstrated that the deletions of the above CBS and CSE genes alter genome expression to different degrees, with a more pronounced effect being exerted by deletion of the CBS gene. Furthermore, the double deletion of both CBS and CSE resulted in a cumulative effect on transcription in the resulting strains. Overall, we found that the obtained deletions affect numerous genes involved in various biological pathways. Specifically, genes involved in the oxidative reduction process, stress-response genes, housekeeping genes, and genes participating in olfactory and reproduction are among the most strongly affected. Furthermore, characteristic differences in the response to the deletions of the studied genes are apparently organ-specific and have clear-cut sex-specific characteristics. Single and double deletions of the three genes responsible for the production of H 2 S helped to elucidate new aspects of the biological significance of this vital physiological mediator.

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Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Genome-wide transcriptional effects of deletions of sulphur metabolism genes in Drosophila melanogaster

et al. 2020

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“…In recent decades, we studied the effects of endogenous and exogenous hydrogen sulfide at the cellular and organism levels (Yurinskaya et al 2020;Shilova et al 2020;Zatsepina et al in press). In our investigation, we explored slow-and fast-releasing H 2 S donors as well as deletions of the genes responsible for H 2 S production and demonstrated a strong anti-inflammatory effect of this gas which ameliorates various manifestations of inflammation including ROS, NO, TNF-α, and interleukin-6.…”

mentioning

confidence: 99%

Possible application of H2S-producing compounds in therapy of coronavirus (COVID-19) infection and pneumonia

Евгеньев

Frenkel²

2020

Cell Stress and Chaperones

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Effects of periodically repeated high‐temperature exposure on the immediate and subsequent fitness of different developmental stages ofPropylaea japonica

Tian

Zhang

Liu

2022

Pest Management Science

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BACKGROUND: Repeated extreme high temperature occurs frequently in summer. Propylaea japonica is a predominant predator in South-East Asia and has been considered as a successful natural enemy to control aphids. However, how repeated extreme high temperature affects the fitness of P. japonica remains unclear. This study evaluated the immediate and subsequent fitness of P. japonica when egg, larva, pupa, and adult were exposed to repeated high temperatures (39, 41, or 43 °C for 3 h exposure duration per day) during several days. RESULTS:The effect of repeated high temperatures on P. japonica fitness was stage-specific: the egg stage was the most sensitive, the larval and pupal stages were moderately resistant and the adult stage was the most resistant to heat. Repeated high temperatures extended the immature developmental time and decreased the sex ratio of eggs treated with these temperatures, compared to control eggs. A temperature of 39 °C had no significant effect on the pre-oviposition period, oviposition period, fecundity (except stress pupa), or longevity compared with the control, but negative carry-over effects above 39 °C on subsequent stages were found. CONCLUSION: Repeated high temperature for consecutive days not only had a significant effect on the immediate survival and developmental time, but also had deleterious effects on the subsequent development and performance of P. japonica.The present study provides valuable information for understanding and utilizing P. japonica to control aphids in challenging environments.

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Age-dependent expression profiles of two adaptogenic systems and thermotolerance in Drosophila melanogaster

Cited by 15 publications

References 67 publications

Genome-wide transcriptional effects of deletions of sulphur metabolism genes in Drosophila melanogaster

Genome-wide transcriptional effects of deletions of sulphur metabolism genes in Drosophila melanogaster

Possible application of H2S-producing compounds in therapy of coronavirus (COVID-19) infection and pneumonia

Effects of periodically repeated high‐temperature exposure on the immediate and subsequent fitness of different developmental stages ofPropylaea japonica

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