Age-dependent modifications in rat hepatocyte antioxidant defense systems

Sanz, Núria; Dı́ez-Fernández, Carmen; Álvarez, Alberto; Cascales, Marı́a

doi:10.1016/s0168-8278(97)80358-3

Cited by 68 publications

(44 citation statements)

References 42 publications

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“…The GPx level of female rats was significantly higher than those of male rats. These results agree with previous finding within different sex and in aging age (10,16,18). According to Rao (18) the age-related changes in the activities of GPx were paralled by a similar change in the relative level of the mRNAs coding for this enzyme.…”

Section: Discussionsupporting

confidence: 92%

“…For this aim, we searched glutathione metabolism in liver of rats. Glutathione is the main non-protein thiol in many enzymatic reactions which are necessary to preserve thiol homeostasis, redox state of the cells and defense against radiation damage (10). We found that rats did not have an ageand sex-associated differences in the liver GSH (Fig.…”

Section: Discussionmentioning

confidence: 63%

“…They suggest that the efects of aging may also depend on the strain of rats. Sanz et al (10) found to be no changes in the GR levels from 21 days to 6 mounths.…”

Section: Discussionmentioning

confidence: 92%

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The effects of exogenous glutathione on reduced glutathione level, glutathione peroxidase and glutathione reductase activities of rats with different ages and gender after whole-body Γ-irradiation

İnal

Akgün

Kahraman

2003

AGE

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Age-and gender-related changes on reduced glutathione (GSH) level, glutathione peroxidase (GPx) and glutathione reductase (GR) activities in the liver of rat exposed to different dose of whole-body g-ray irradiation were determined. In addition, the effect of administration of exogenous GSH on endogenous GSH levels, GPx and GR activities was investigated. For this aim, male and female rats aged 1 and 5 moths were divided into two groups as g-ray and g-ray+GSH. Both groups were again divided into four groups as irradiated with 2, 4, 6 and 8 Gy doses. GSH level and GPx activity did not change with age while GR activity was decreased with age. Gender-dependent changes in GPx and GR activities were observed, but GSH values were not affect by sex. GSH levels, GPx and GR activities were not obsewed dose-associated changes of g-irradiation. GSH level and GPx activity in the 8Gy group were increased by GSH. GR activities of old male rats were found to be increased by glutathione in the 6 and 8Gy groups. These results indicate that radiation and administration of exogenous GSH affect gender-and age-dependent GSH level, GPx and GR activities in the rats.

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Section: Discussionsupporting

confidence: 92%

Section: Discussionmentioning

confidence: 63%

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The effects of exogenous glutathione on reduced glutathione level, glutathione peroxidase and glutathione reductase activities of rats with different ages and gender after whole-body Γ-irradiation

İnal

Akgün

Kahraman

2003

AGE

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“…Studies have examined the GSH or GSH/GSSG in old and young animals and have found an increase in GSSG (38,46). We examined the level of GSH and GSSG with respect to the increase in endothelial cell size and protein content and found that endothelial cells maintained a reducing environment, even at the end of their replicative life (Fig.…”

Section: Discussionmentioning

confidence: 99%

Endothelial cells maintain a reduced redox environment even as mitochondrial function declines

Carlisle

Rhoads

et al. 2002

American Journal of Physiology-Cell Physiology

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. Endothelial cells maintain a reduced redox environment even as mitochondrial function declines. Am J Physiol Cell Physiol 283: C1675-C1686, 2002. First published August 22, 2002 10.1152/ajpcell.00092.2002 are an endothelial model of replicative senescence. Oxidative stress, possibly due to dysfunctional mitochondria, is believed to play a key role in replicative senescence and atherosclerosis, an age-related vascular disease. In this study, we determined the effect of cell division on genomic instability, mitochondrial function, and redox status in HUVECs that were able to replicate for ϳ60 cumulative population doublings (CPD). After 20 CPD, the nuclear genome deteriorated and the protein content of the cell population increased. This indicated an increase in cell size, which was accompanied by an increase in oxygen consumption, ATP production, and mitochondrial genome copy number and ϳ10% increase in mitochondrial mass. The antioxidant capacity increased, as seen by an increase in reduced glutathione, glutathione peroxidase, GSSG reductase, and glucose-6-phosphate dehydrogenase. However, by CPD 52, the latter two enzymes decreased, as well as the ratio of mitochondrial-to-nuclear genome copies, the mitochondrial mass, and the oxygen consumption per milligram of protein. Our results signify that HUVECs maintain a highly reducing (GSH) environment as they replicate despite genomic instability and loss of mitochondrial function. replicative senescence; glutathione; cell size changes; genomic instability; human umbilical vein endothelial cells HUMAN UMBILICAL VEIN ENDOTHELIAL CELLS (HUVECs) replicate a finite number of times in culture and are an endothelial model of replicative senescence. Reactive oxygen species (ROS) and oxidative damage to DNA (24), protein (7), and lipid (45) increase with age and have been implicated in aging and replicative senescence (19,48). Oxidative stress has also been implicated in atherosclerosis, an age-related vascular disease, and homocysteine, a contributing factor to atherosclerosis that induces oxidative stress, accelerates endothelial senescence in culture (52). Atherosclerosis is hypothesized to be the result of a "response to injury" of the endothelium. The endothelium must constantly respond to changes in the vascular environment and experiences frequent insults from mechanical, hemodynamic, and inflammatory stresses, all of which enhance oxygen free radical production. In response to injury, endothelial cells must replicate to repair blood vessels. Telomere length is shorter in endothelial cells derived from vessels where there is greater hemodynamic stress, and presumably more endothelial cell replication to repair injury, than in cells isolated from areas of lower stress (10). Injury induced by balloon catheter denudation of rabbit carotid arteries has been shown to result in the accumulation of senescent endothelial cells (18). Studies of the endothelium at atherosclerotic lesions (9, 50) and in vessels from elderly people (30, 50) have detected multinucleated cells ...

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“…Numerous reports [1][2][3][4][5] have made it clear that the toxicity of oxidized edible oil is due mainly to oxidation products from unsaturated fatty acids. The ingredient standard for commercially processed foods, Japan, established the chemical properties of oil and fat at AV 3 and POV 30 6) .…”

Section: Introductionmentioning

confidence: 99%

Chemical Properties and Cytotoxicity of Thermally Oxidized Oil

et al. 2008

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Heated frying oils with different chemical properties in terms of AV (acid value), POV (peroxide value), COV (carbonyl value), and contents of polar compounds (PC) and triacylglycerol (TG), as well as color and odor, were obtained. Male Wistar rats were fed ad libitum for 12 weeks a powdered diet (AIN93G; no fat) containing 7 wt% of fresh oil (control) or one of the frying oils described above. The rats were subjected to anthropometric measurements, hematological analyses, and observations of the liver and kidneys. All of the rats grew well, and no gross symptoms attributable to the experimental oils were observed. However, the rats fed a diet containing the heated oil developed apparent liver damage to different degrees regardless of the chemical properties of the ingested oils. Thus, it was suggested that the chemical properties evaluated here had little to do with the cytotoxicity of heated oil, although the properties express quality of oil. Volatile compounds seem to be major candidates for the toxic agents in heated oil because oils with rancid and deteriorated odor show strong toxicity.

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Age-dependent modifications in rat hepatocyte antioxidant defense systems

Cited by 68 publications

References 42 publications

The effects of exogenous glutathione on reduced glutathione level, glutathione peroxidase and glutathione reductase activities of rats with different ages and gender after whole-body Γ-irradiation

The effects of exogenous glutathione on reduced glutathione level, glutathione peroxidase and glutathione reductase activities of rats with different ages and gender after whole-body Γ-irradiation

Endothelial cells maintain a reduced redox environment even as mitochondrial function declines

Chemical Properties and Cytotoxicity of Thermally Oxidized Oil

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