The effects of exogenous glutathione on reduced glutathione level, glutathione peroxidase and glutathione reductase activities of rats with different ages and gender after whole-body Γ-irradiation

İnal, Mine; Akgün, Asiye; Kahraman, Ahmet

doi:10.1007/s11357-003-0005-8

Cited by 8 publications

(7 citation statements)

References 18 publications

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“…Endogenous GSH levels contribute to the maintenance of cell homeostasis by eliminating free radicals because of physical or chemical injuries (Erden Inal, Akgün, & Kahraman, ). In this work, there was an augmentation of GSH levels and a reduced activity of GPx in the group exposed to the higher dose of statin, indicating that the major source of detoxification in this study is mediated by increased catalase activity.…”

Section: Discussionmentioning

confidence: 99%

Ascorbic acid supplementation ameliorates testicular hormonal signaling, sperm production and oxidative stress in male rats exposed to rosuvastatin during pre‐puberty

Leite

Barros

Martins

et al. 2018

J of Applied Toxicology

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Dyslipidemias are occurring earlier in the population due to the augmentation of obesity. Rosuvastatin reduces cholesterol and triglycerides; however, previous studies have shown that it may affect male reproduction. Ascorbic acid (AA), an antioxidant compound, plays a protective role in the male reproductive system. This study aimed to evaluate whether pre‐pubertal exposure to rosuvastatin may impair testicular structure and antioxidant status in male rats and if supplementation with AA may alleviate these damages. Male rats were randomly divided into six experimental groups (n = 10) on postnatal day (PND) 23 and received the different treatments by gavage from PND 23 to 53. The experimental groups received vehicle (saline solution 0.9%), 3 or 10 mg/kg/day of rosuvastatin diluted in saline solution 0.9%, supplementation with 150 mg/day of AA, 3 mg/kg/day of rosuvastatin in association with 150 mg/day of AA or 10 mg/kg/day of rosuvastatin associated with 150 mg/day of AA. Testicular parameters were assessed on PND 53 and 110. There were diminished androgen receptors staining in the Sertoli cells and increased germ cell death in rosuvastatin‐exposed groups, in both periods. Spermatids showed lower estrogen alpha‐receptors staining in the group exposed to 10 mg of statin at adulthood. There were androgen depletion and increased lipid peroxidation and catalase activity in statin‐exposed groups. Rosuvastatin exposure during pre‐puberty impaired testicular structure, steroid receptor distribution and increased oxidative stress; however, AA was able to ameliorate the impairment provoked by statin exposure.

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Section: Discussionmentioning

confidence: 99%

Ascorbic acid supplementation ameliorates testicular hormonal signaling, sperm production and oxidative stress in male rats exposed to rosuvastatin during pre‐puberty

Leite

Barros

Martins

et al. 2018

J of Applied Toxicology

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“…Gpx activity was measured by adding 0.2 ml of Tris-HCl buffer, 0.2 ml of GSH, 0.1 ml of H 2 O 2 , 0.2 ml of homogenate, and sodium azide (0.1 ml) to the reaction tube. The reaction tube was centrifuged for 10 min at 3,000 × g. Then, cell culture supernatant (0.2 ml) and Ellman's reagent (0.1 ml) were added to the reaction tube, and the final absorbance was measured at 340 nm ( 25 ).…”

Section: Methodsmentioning

confidence: 99%

“…SOD activity was determined by adding cell culture supernatant (0.1 ml), nitro blue tetrazolium (0.3 ml), NADH (0.2 ml) and sodium phosphate buffer (1.2 ml). The final absorbance was measured at 560 nm ( 25 ). Catalase activity was determined by adding phosphate buffer (500 µl), cell culture supernatant (500 µl) and H 2 O 2 (500 µl).…”

Section: Methodsmentioning

confidence: 99%

“…Catalase activity was determined by adding phosphate buffer (500 µl), cell culture supernatant (500 µl) and H 2 O 2 (500 µl). Then, TiOSO 4 (500 µl) was added to the reaction tube, and the final absorbance was measured at 420 nm ( 25 ). AChE activity was determined by the addition of acetylcholine (0.02 ml), cell culture supernatant (0.02 ml), DTNB (0.1 ml) and phosphate buffer (3 ml) into the reaction tube.…”

Section: Methodsmentioning

confidence: 99%

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Protective effects of taurine against inflammation, apoptosis, and oxidative stress in brain injury

et al. 2018

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The protective effect of taurine against inflammation, apoptosis and oxidative stress in traumatic brain injury was investigated in the present study. Taurine is a non-proteogenic and essential amino acid in animals. It plays a critical nutritional role in brain cell growth, differentiation, and development. Taurine is involved in regeneration and neuroprotection in the injured nervous system, and is an effective antioxidant against lead-, cadmium-, and exercise-induced oxidative stress. Astrocytes and neuron cells were co-cultured and cells were treated with different concentrations of taurine (100, 200 and 300 mg/l) for 72 h, and the levels of reactive oxygen species, malondialdehyde, reduced glutathione, glutathione peroxidase, superoxide dismutase, catalase, acetylcholinesterase, tumor necrosis factor-α, interleukin-6, caspase-3, p53, B-cell lymphoma 2 and Bcl-2-associated X protein were determined. These inflammatory, apoptotic, and oxidative stress markers were substantially increased in injured cells, and returned to normal levels following taurine supplementation. Thus, taurine supplementation may be effective against oxidative stress, apoptosis, and inflammation in injured brain cells.

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“…Glutathione peroxidase (Gpx), superoxide dismutase (SOD), catalase and acetylcholinesterase (AChE) enzyme activities in the heart tissue homogenate were determined using spectrophotometry . Reduced glutathione (GSH) content in the heart tissue homogenate was determined by measuring the final product at 405 nm …”

Section: Methodsmentioning

confidence: 99%

Omega‐6 fatty acids down‐regulate matrix metalloproteinase expression in a coronary heart disease‐induced rat model

et al. 2018

Int J Experimental Path

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Summary The present study investigated the therapeutic potential of omega‐6 fatty acids, according to their effects on antioxidant markers and matrix metalloproteinases (MMPs), in coronary heart disease‐induced rats. Rats were grouped into group I (sham control), group II (control), group III (0.5 g/kg bwt of omega‐6 fatty acids) and group IV (1 g/kg bwt of omega‐6 fatty acids). Reactive oxygen species (ROS), malondialdehyde (MDA), superoxide dismutase (SOD), reduced glutathione (GSH), catalase, glutathione peroxidase (Gpx) and acetylcholinesterase (AChE) enzyme activities were determined. ROS and MDA were substantially reduced, whereas SOD, catalase, Gpx and AChE were significantly increased, following supplementation with omega‐6 fatty acids. MMP‐2 mRNA expression was drastically increased by 95% in group II. Treatment significantly reduced MMP‐2 mRNA expression by 12.3% and 26.7% in groups III and IV respectively. MMP‐9 mRNA expression drastically increased, by 121%, in group II. Treatment significantly reduced MMP‐9 mRNA expression by 22.6% and 29.4% in groups III and IV respectively. MMP‐2 protein expression was drastically increased, by 81%, in group II. Treatment significantly reduced MMP‐2 protein expression by 9.4% and 26% in groups III and IV respectively. MMP‐9 protein expression was drastically increased, by 100%, in group II. Treatment significantly reduced MMP‐9 protein expression by 18.9% and 26.9% in groups III and IV respectively. In summary, the consumption of omega‐6 fatty acids significantly decreased MDA and ROS, while SOD, catalase, GHS, Gpx and AChE were increased. Furthermore, omega‐6 fatty acids significantly downregulated MMP‐2 and MMP‐9 expression in our coronary heart disease‐induced rat model.

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The effects of exogenous glutathione on reduced glutathione level, glutathione peroxidase and glutathione reductase activities of rats with different ages and gender after whole-body Γ-irradiation

Cited by 8 publications

References 18 publications

Ascorbic acid supplementation ameliorates testicular hormonal signaling, sperm production and oxidative stress in male rats exposed to rosuvastatin during pre‐puberty

Ascorbic acid supplementation ameliorates testicular hormonal signaling, sperm production and oxidative stress in male rats exposed to rosuvastatin during pre‐puberty

Protective effects of taurine against inflammation, apoptosis, and oxidative stress in brain injury

Omega‐6 fatty acids down‐regulate matrix metalloproteinase expression in a coronary heart disease‐induced rat model

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