Age estimation of blood stains by hemoglobin derivative determination using reflectance spectroscopy

Bremmer, Rolf H.; Nadort, Annemarie; Leeuwen, Ton G. van; Gemert, Martin J. C. van; Aalders, Maurice C. G.

doi:10.1016/j.forsciint.2010.07.034

Cited by 111 publications

(67 citation statements)

References 23 publications

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“…Next to this, the chemical composition of biological traces may change in time. Although these changes can be used for age estimation purposes [43,54], the influence of environmental conditions like temperature, humidity, precipitations and light should be studied. For example the influence of temperature and humidity on the aging of blood stains is shown by Bremmer et al [55].…”

Section: Typical Challengesmentioning

confidence: 99%

“…Many applications currently performed with conventional spectroscopy may also be enhanced with a spatial component using HSI, similar to the way presented for the identification [17,64,65], and age estimation of blood stains [18,54,[65][66][67][68], as shown in Figure 9. In these applications, the added spatial information is crucial, as the blood stain pattern may reveal useful information for the reconstruction of a crime [69].…”

Section: Future Applicationsmentioning

confidence: 99%

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Hyperspectral imaging for non-contact analysis of forensic traces

Edelman

Gaston

Leeuwen

et al. 2012

Forensic Science International

250

116

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Hyperspectral imaging (HSI) integrates conventional imaging and spectroscopy, to obtain both spatial and spectral information from a specimen. This technique enables investigators to analyze the chemical composition of traces and simultaneously visualize their spatial distribution. HSI offers significant potential for the detection, visualization, identification and age estimation of forensic traces. The rapid, non-destructive and non-contact features of HSI mark its suitability as an analytical tool for forensic science. This paper provides an overview of the principles, instrumentation and analytical techniques involved in hyperspectral imaging. We describe recent advances in HSI technology motivating forensic science applications, e.g. the development of portable and fast image acquisition systems. Reported forensic science applications are reviewed. Challenges are addressed, such as the analysis of traces on backgrounds encountered in casework, concluded by a summary of possible future applications.

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Section: Typical Challengesmentioning

confidence: 99%

Section: Future Applicationsmentioning

confidence: 99%

Hyperspectral imaging for non-contact analysis of forensic traces

Edelman

Gaston

Leeuwen

et al. 2012

Forensic Science International

250

116

View full text Add to dashboard Cite

show abstract

“…Bloodstains are often encountered at scenes of violent crime and have significant forensic value [1]. Blood is one of the most commonly encountered types of biological evidence [2].…”

Section: Introductionmentioning

confidence: 99%

“…Considerable previous research has explored the detection and identification of blood stains using a variety of techniques, including RNA analysis [7,8]; microscopy techniques [9], and spectroscopic methods [10][11][12][13]. Recent research has explored the use of non-contact reflectance spectroscopy to detect blood stains with high levels of specificity [1]. This led to the use of visible wavelength hyperspectral imaging (HSI), which was first reported for the detection and age determination of horse blood stains between 442 nm and 585 nm as proof of concept research [14].…”

Section: Introductionmentioning

confidence: 99%

A comparison of visible wavelength reflectance hyperspectral imaging and Acid Black 1 for the detection and identification of blood stained fingerprints

Cadd

Beveridge

et al. 2016

Science & Justice

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Bloodstains are often encountered at scenes of violent crime and have significant forensic value for criminal investigations. Blood is one of the most commonly encountered types of biological evidence and is the most commonly observed fingerprint contaminant. Presumptive tests are used to test blood stain and blood stained fingerprints are targeted with chemical enhancement methods, such as acid stains, including Acid Black 1, Acid Violet 17 or Acid Yellow 7. Although these techniques successfully visualise ridge detail, they are destructive, do not confirm the presence of blood and can have a negative impact on DNA sampling. A novel application of visible wavelength hyperspectral imaging (HSI) is used for the noncontact, non-destructive detection and identification of blood stained fingerprints on white tiles both before and after wet chemical enhancement using Acid Black 1. The identification was obtained in a non-contact and non-destructive manner, based on the unique visible absorption spectrum of haemoglobin between 400 and 680 nm. Results from the exploration of the selectivity of the setup to detect blood against ten other non-blood protein contaminants are also presented. A direct comparison of the effectiveness of HSI with chemical enhancement using Acid Black 1 on white tiles is also presented.

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“…Many approaches have been explored for the age determination of blood stains, including hemoglobin derivative determination [1], hyperspectral imaging [2], relative RNA ratios method [3], atomic force microscopy method [4]. However, sometimes these methods are not feasible to operate.…”

Section: Introductionmentioning

confidence: 99%

Research on the relationship between the age of blood stains and the bending rigidity of blooded cotton fabric

Cao¹,

Ma²,

Zhao³

et al. 2015

Proceedings of the 5th International Conference on Advanced Design and Manufacturing Engineering

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Abstract. Blood stains often appear at the scene of the crime. The age determination of blood stains can provide important information to speculate the time of crime. The relationship between the age of blood and the bending rigidity of blooded cotton fabric was investigated experimentally via the use of FAST-2 bending tester. Measure the bending length of blooded fabric at different time and calculate the bending rigidity, then the relationship between the age of blood and the bending rigidity is obtained through curve fitting. The result shows that the bending rigidity of blooded fabric is various with the increase in the age of blood stains and this relationship can be used to speculate the age of blood stains.

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Age estimation of blood stains by hemoglobin derivative determination using reflectance spectroscopy

Cited by 111 publications

References 23 publications

Hyperspectral imaging for non-contact analysis of forensic traces

Hyperspectral imaging for non-contact analysis of forensic traces

A comparison of visible wavelength reflectance hyperspectral imaging and Acid Black 1 for the detection and identification of blood stained fingerprints

Research on the relationship between the age of blood stains and the bending rigidity of blooded cotton fabric

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