Age-related activity of catalase in different genotypes of drosophila melanogaster

Durusoy, Mübeccel; Dırıl, Nuran; Bozcuk, A. Nihat

doi:10.1016/0531-5565(94)00033-y

Cited by 16 publications

(5 citation statements)

References 23 publications

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“…The catalase gene represents a key component of the antioxidative defense network. In adult flies, catalase levels have been reported to either drop slightly (Durusoy et al, 1995;Nicolosi et al, 1973;Sohal et al, 1990) or remain largely unchanged (Niedzwiecki et al, 1992) as a function of age. On the other hand, during development catalase levels vary in a dynamic fashion.…”

Section: Introductionmentioning

confidence: 99%

Catalase expression inDrosophila melanogaster is responsive to ecdysone and exhibits both transcriptional and post-transcriptional regulation

Radyuk

Klichko

Orr

2000

Arch. Insect Biochem. Physiol.

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In the present study, we have examined catalase protein and mRNA levels and the factors that may regulate catalase expression in Drosophila melanogaster during development. Both mRNA and protein changes are in general accord with variations in ecdysteroid titer during development. Differences in mRNA and protein accumulation profiles, particularly in embryos and young adults, suggest that catalase may be regulated at both transcriptional and post-transcriptional levels. It was possible to induce catalase expression by administering exogenous 20-hydroxyecdysone (Ec) in culture at certain stages of development (usually at time points corresponding to previously observed hormone and catalase peaks). Experiments with exogenous administration of Ec, cycloheximide, and actinomycin D suggest a complex interplay of factors affecting catalase expression. In cultured third instar larvae, superinduction of catalase occurred in the presence of both Ec and cycloheximide. If ecdysteroid production was suppressed prior to antibiotic treatment by temperature upshift of the conditional mutant dre4(e55), superinduction occurred mostly at the protein level. In cultured adult abdomens, we observed induction by Ec and superinduction in the presence of hormone and translation or transcription inhibitors. Unlike what was observed in larvae, superinduction of catalase protein was dramatically more pronounced in control flies.

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Section: Introductionmentioning

confidence: 99%

Catalase expression inDrosophila melanogaster is responsive to ecdysone and exhibits both transcriptional and post-transcriptional regulation

Radyuk

Klichko

Orr

2000

Arch. Insect Biochem. Physiol.

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“…Although virgin females were used in the study; according to OSI, it is determined that it causes high stress by feeding females with PSM and is even likely to produce fatal effects (high oxidative stress) of HFD + 2 g PSM ( (Missirlis et al, 2003;Güneş, 2016a and b). For example, in some studies, it was reported that CAT and GR amounts decreased and GST activity changed in Drosophila due to xenobiotics and aging (Durusoy et al, 1995;Fışkın and Asma, 1996;Yeşilada and Gelegen, 2000).…”

Section: Discussionmentioning

confidence: 99%

Evaluation of the Pumpkin Seed Membrane as a Nutrient in the Fruit Fly Model, Fed With High Fat Diet

Güneş

Bayram

2021

Selçuk Üniversitesi Fen Fakültesi Fen Dergisi

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The pumpkin seed membrane (PSM), which is high in water retention in the study, was recycled as waste, intended to be used in terms of target-non-target creatures and to determine its effect on resistance formation. Nowadays, when fat intake is steadily increasing through nutrition, increased use of fat makes an individual vulnerable to stress. The study determined the effect of additional taken waste/nutrients on oxidative stress in obese individuals. The fatty diet and pumpkin seed effect were trialed in the model organism in the study. The amount of Malondialdehyde (MDA) and total oxidation (TOS) and total antioxidant effect (TAS) and glutathione-S-transferase (GST) activity in larvae, pupae, and adult of Drosophila melanogaster were calculated by adding different proportions of PSM (0.05-2 g) and fat (20%) to the diet. According to the data obtained: It was determined that the period with the highest oxidative stress index (OSI) is the pupae (pupae> larvae> female> male), the amount of lipid peroxidation (MDA) is higher in females, moreover, the third larvae is the most resistant stage. The results have shown that waste of seed mambrane can't be useful in vivo as a nutrient due to usage limitation.

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“…The most convincing evidence for the involvement of free radicals in aging comes from a recent study that demonstrates life span increases (14 to 34%) in transgenic Drosophila that simultaneously overexpress both CAT and Cu/Zn-SOD (33). These transgenic lines show a 25 to 37% increase in Cu/Zn-SOD activity and a 45 to 70% increase in CAT activity.…”

Section: Overexpression Of Antioxidant Enzymes In Transgenic Drosophilamentioning

confidence: 97%

New directions for studying the role of free radicals in aging

Pahlavani

Remmen

1997

AGE

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Oxidative damage caused by free radicals in vivo is believed to play an important role in the etiology of aging and age-associated degenerative diseases. The most direct evidence supporting this theory is the recent finding that the transgenic Drosophila that overexpress the antioxidant enzymes catalase and superoxide dismutase exhibit an increase in life span. Although the increase in life span in Drosophila by these enzymes is certainly important, the next logical direction is to demonstrate whether increased antioxidant protection occurs similarly in mammals. Several transgenic mouse models that overexpress antioxidant enzymes are currently available. However, one major shortcoming in using these transgenic mice is the difficulty of producing antioxidant overexpression in more than a few tissues. Despite the potential shortcomings of using transgenic mice, these animals provide a unique system in which individual components of a complex system, such as the antioxidant defense system, can be modulated and examined independently. Transgenic mice are therefore potentially powerful tools to study the role of various components of the antioxidant system in the aging process.A parallel direction in the study of free radical roles in aging is to investigate the modulation of transcription factors by oxidative stress. Among these, the transcription factors, NF-KB and AP-1 are implicated in oxidative stress. The activities of these oxidative stress-response transcription factors are regulated by upstream signaling molecules, which involve a cascade of phosphorylation and dephosphorylation events leading to their activation. In this article, we review recent studies that use molecular approaches to investigate the biological role of oxidant stress. Each of these studies potentially provide new insights into the roles of free radicals and free radical damage in the aging process.

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Age-related activity of catalase in different genotypes of drosophila melanogaster

Cited by 16 publications

References 23 publications

Catalase expression inDrosophila melanogaster is responsive to ecdysone and exhibits both transcriptional and post-transcriptional regulation

Catalase expression inDrosophila melanogaster is responsive to ecdysone and exhibits both transcriptional and post-transcriptional regulation

Evaluation of the Pumpkin Seed Membrane as a Nutrient in the Fruit Fly Model, Fed With High Fat Diet

New directions for studying the role of free radicals in aging

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