Age‐related changes in Ki‐67 and DCX expression in the BALB/ c mouse (<i>Mus Musculus</i>) brain

Nkomozepi, Pilani; Mazengenya, Pedzisai; Ihunwo, Amadi O.

doi:10.1016/j.ijdevneu.2018.11.005

Cited by 9 publications

(10 citation statements)

References 60 publications

(105 reference statements)

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“…S5D). Consistent with previous observations (Nkomozepi, Mazengenya, and Ihunwo 2019), the number of DCX + cells significantly decreased with age, whereas the number of either GFP + cells did not significantly change. Taken together, our data show that, within a six-week period, apical NSCs generate few basal cells in the SVZ with antigenic characteristics of NSCs and rare OB interneurons.…”

Section: Resultssupporting

confidence: 92%

“…S5D). Consistent with previous observation (Nkomozepi, Mazengenya, and Ihunwo 2019), the number of DXC + cells significantly decreased with age, whereas the number of either GFP + cells did not significantly change. Since we have previously shown that most OB adult born interneurons derive from NSCs in the V-SVZ tagged with a murine minimal GFAP promoter (Weber et al 2011), we conclude that basal NSCs, but not apical, are the main source of new OB neurons.…”

Section: Basal Nscs Derive From Apical Nscs and Are The Main Contribusupporting

confidence: 92%

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A novel stem cell type at the basal side of the subventricular zone maintains adult neurogenesis

Baur

Abdullah

Mandl

et al. 2020

Preprint

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Neural stem cells (NSCs) in the ventricular-subventricular zone (V-SVZ) contribute to olfaction by being the origin of most adult-born olfactory bulb (OB) interneurons. The current consensus maintains that adult NSCs are radial glialike progenitors apically contacting the lateral ventricle and generating intermediate progenitors migrating at the basal V-SVZ. Whether basal NSCs are present in the V-SVZ is unknown. We here used genetic tagging of NSCs in vivo and additional labelling approaches to reveal that basal NSCs lacking apical attachment represent the largest NSC type in the postnatal V-SVZ from birth onwards. Despite dividing faster than their apical counterpart, basal NSCs still undergo long-term self-renewal and quiescence. Unlike apical NSCs, they are largely devoid of primary cilia and Prominin-1, Nestin and glial fibrillary acidic protein (GFAP) immunoreactivity. Six weeks after viral tagging of apical cells, few descendant cells were detected in the basal V-SVZ, including Sox9+ progenitors and GFAP+ astrocytes, and very rare new neurons in the OB, indicating that adult-born OB neurons originate from basal and not apical NSCs. Consistent with this, we found that pregnancy, a physiological modulator of adult OB neurogenesis, selectively increases the number of basal but not apical NSCs. Lastly, we find that apical NSCs display the highest levels of Notch activation in the neural lineage, and that selective apical downregulation of Notch-signaling effector Hes1 decreases Notch activation while increasing proliferation across the V-SVZ. Thus, apical NSCs act essentially as neurogenesis gatekeepers by modulating Notch-mediated lateral inhibition of proliferation in the adult V-SVZ.Graphical AbstractHighlightsBasal NSCs are the most abundant stem cell type in the adult V-SVZ from birth onwards.Apical and basal NSCs display distinct characteristics and cell cycle progression dynamics.Apical NSCs are not the main source of newly generated adult OB interneurons.Apical NSCs regulate intermediate progenitor proliferation by orchestrating Notch-mediated lateral inhibition.

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Section: Resultssupporting

confidence: 92%

Section: Basal Nscs Derive From Apical Nscs and Are The Main Contribusupporting

confidence: 92%

A novel stem cell type at the basal side of the subventricular zone maintains adult neurogenesis

Baur

Abdullah

Mandl

et al. 2020

Preprint

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“…Here, we used an antibody against Ki-67, a nuclear protein expressed only during cell division, to examine postnatal hippocampal proliferation, while neurogenesis was evaluated with DCX, a microtubule-associated protein expressed in neuroblasts/migrating neurons [ 32 ]. Unlike Ki-67, which was not immunodetected in the postnatal hippocampus at any age in either group, DCX was observed mainly in the DG and CA subfields in younger DS and NTD cases (38 to 53 weeks; Figure 7 A,B).…”

Section: Resultsmentioning

confidence: 99%

Postnatal Cytoarchitecture and Neurochemical Hippocampal Dysfunction in Down Syndrome

Moreno

Utagawa

Arva

et al. 2021

JCM

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Although the prenatal hippocampus displays deficits in cellular proliferation/migration and volume, which are later associated with memory deficits, little is known about the effects of trisomy 21 on postnatal hippocampal cellular development in Down syndrome (DS). We examined postnatal hippocampal neuronal profiles from autopsies of DS and neurotypical (NTD) neonates born at 38-weeks’-gestation up to children 3 years of age using antibodies against non-phosphorylated (SMI-32) and phosphorylated (SMI-34) neurofilament, calbindin D-28k (Calb), calretinin (Calr), parvalbumin (Parv), doublecortin (DCX) and Ki-67, as well as amyloid precursor protein (APP), amyloid beta (Aβ) and phosphorylated tau (p-tau). Although the distribution of SMI-32-immunoreactive (-ir) hippocampal neurons was similar at all ages in both groups, pyramidal cell apical and basal dendrites were intensely stained in NTD cases. A greater reduction in the number of DCX-ir cells was observed in the hippocampal granule cell layer in DS. Although the distribution of Calb-ir neurons was similar between the youngest and oldest NTD and DS cases, Parv-ir was not detected. Conversely, Calr-ir cells and fibers were observed at all ages in DS, while NTD cases displayed mainly Calr-ir fibers. Hippocampal APP/Aβ-ir diffuse-like plaques were seen in DS and NTD. By contrast, no Aβ1–42 or p-tau profiles were observed. These findings suggest that deficits in hippocampal neurogenesis and pyramidal cell maturation and increased Calr immunoreactivity during early postnatal life contribute to cognitive impairment in DS.

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“…[12] A study found that expression of Ki-67 sharply decreased with advancing age or life history stages in the mouse brain . [13] In the present investigation, we concentrated on studying the promising role of selenium in reversing the degenerative effects of cellular senescence on submandibular and sublingual salivary glands which ensures this antioxidant capability in regaining the normal histological architecture and subsequently functions of these glands.…”

Section: Introductionmentioning

confidence: 99%

Histological and Immunohistochemical Study of Selenium Regenerative Effect on Submandibular and Sublingual Glands of Aging Rats

Elsaied

2019

Egyptian Dental Journal

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Background:The role of Selenium as an antioxidant has become a point of interest for its importance in prevention of cancer and other systemic diseases especially those associated with aging. Objectives:The aim of the present investigation was to investigate the effect of Selenium supplementation on the submandibular and sublingual salivary glands of aging rats through:1-Histological examination. 2-Immunohistochemical localization of Ki67. Materials and Methods:Thirty young adult male albino rats with body weight ranged from 80-110 gram and of 2 month age were used and divided into the following groups. Group I (control group): Consisted of 20 rats, subdivided into 2 subgroups. Group I.1:10 animals were sacrificed after one month to serve as young controls. Group I.2:10 animals were left till the end of the experiment to serve as old controls. Group II: 10 animals received Selenium 150 µg/kg diet for the whole experimental period. The experiment lasted for 9 months.Results: histological results revealed that aging caused devastating effects on the histological structure of submandibular and sublingual salivary glands. Selenium markedly modulated the action of aging process and increased the regenerative capacity of submandibular and sublingual glands. According to expression intensity of Ki67 stain in submandibular and sublingual glands, there was statistically significant difference between young, selenium and old groups. In general, expression intensity of Ki67 stain was the highest in the young control group followed by selenium group and lowest in old control group. Also, ki67 expression was significantly higher in sublingual than submandibular glands. Conclusions:Selenium was documented to have a powerful and beneficial antiaging effect. This faced lights around the promising role of selenium in the new era of regenerative medicine. Sublingual gland was more resistant to regressive changes of aging than submandibular gland.

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Age‐related changes in Ki‐67 and DCX expression in the BALB/ c mouse (Mus Musculus) brain

Cited by 9 publications

References 60 publications

A novel stem cell type at the basal side of the subventricular zone maintains adult neurogenesis

A novel stem cell type at the basal side of the subventricular zone maintains adult neurogenesis

Postnatal Cytoarchitecture and Neurochemical Hippocampal Dysfunction in Down Syndrome

Histological and Immunohistochemical Study of Selenium Regenerative Effect on Submandibular and Sublingual Glands of Aging Rats

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