Age-related changes in mature CD4+ T cells: cell cycle analysis

Hale, Timothy J.; Richardson, Bruce C.; Sweet, Leonard I.; McElligott, David L.; Riggs, James E.; Chu, E B; Glynn, Jacqueline M.; Lafrenz, D; Ernst, David; Rochford, Rosemary; Hobbs, Monte V.

doi:10.1016/s0008-8749(03)00007-8

Cited by 13 publications

(15 citation statements)

References 32 publications

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“…Quadri et al (22) attributed the dysregulation of cell cycle with aging in human T lymphocytes to the lower Cdk1 activity and lower protein level of Ccnb1 and Cdk1/Ccnb1 complex compared with those of young. A decline in the mRNA and protein levels of Ccnd2 at 32-36 h after stimulation with anti-CD3 and anti-CD28 has also been proposed to be responsible for decreased proliferative response in aged CD4 ϩ T cells from mice (38). In this study, we did not observe a significant age-related difference in expression levels of these cell cycle-related genes.…”

Section: Mip-1b Gm-csf Tnf-␣ Il-2 Il-4 and Egr2 Exhibited The Hicontrasting

confidence: 50%

Age and Vitamin E-Induced Changes in Gene Expression Profiles of T Cells

Han

Adolfsson

Lee

et al. 2006

The Journal of Immunology

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T cells are vulnerable to age-associated changes. Vitamin E has been shown to improve T cell functions in the old. We studied gene expression profiles of T cells to better understand the underlying mechanisms of age and vitamin E-induced changes in T cell function. Young and old C57BL mice were fed diets containing 30 (control) or 500 (supplemented) ppm of vitamin E for 4 wks. Gene expression profiles of T cells were assessed using microarray analysis with/without anti-CD3/anti-CD28 stimulation. Genes associated with cytokines/chemokines, transcriptional regulation, signal transduction, cell cycle, and apoptosis were significantly up-regulated upon stimulation. Higher SOCS3 and lower growth factor independent 1 (Gfi-1) expression in old T cells may contribute to age-associated decline in proliferation. Higher Gadd45 and lower Bcl2 expression may contribute to increased apoptosis in old T cells. Vitamin E supplementation resulted in higher expression of genes involved in cell cycle regulation (Ccnb2, Cdc2, Cdc6) in old T cells. Vitamin E supplementation resulted in higher up-regulation of IL-2 expression in young and old T cells and lower up-regulation of IL-4 expression in old T cells following stimulation. These findings suggest that aging has significant effects on the expression of genes associated with signal transduction, transcriptional regulation, and apoptosis pathways in T cells, and vitamin E has a significant impact on the expression of genes associated with cell cycle and Th1/Th2 balance in old T cells. Further studies are needed to determine whether these changes are due to the effects of aging at a single-cell level or to the shift in the ratio of naïve:memory T cells with age.

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Section: Mip-1b Gm-csf Tnf-␣ Il-2 Il-4 and Egr2 Exhibited The Hicontrasting

confidence: 50%

Age and Vitamin E-Induced Changes in Gene Expression Profiles of T Cells

Han

Adolfsson

Lee

et al. 2006

The Journal of Immunology

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“…The aging of the immune system (immunosenescence) is associated with dramatic reductions in immune responsiveness as well as functional dysregulation (Burns, 2004). This translates into less effective innate and adaptive immune responses, increased reactivity against self-antigens (autoimmunity) and decreased incidences of infectious diseases and cancer (Hale et al, 2002). As shown in Tables 2 and 3, the decreased thymus and spleen indices, and macrophage function in aged mice was a good indicator of age-induced decline in immune function.…”

Section: Discussionmentioning

confidence: 99%

Effect of the Lycium barbarum polysaccharides on age-related oxidative stress in aged mice

Li¹,

Ma²,

Liu³

2007

Journal of Ethnopharmacology

251

115

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“…The following fluorochromeconjugated monoclonal antibodies were obtained from BD PharMingen (San Diego, CA): FITC-conjugated anti-human CD70, CD2, or isotype-matched controls; phycoerythrin (PE)-conjugated anti-CD2, CD4, and CD8; and CyChromeconjugated anti-HLA-DR, CD2, and isotype controls. Staining and multicolor flow cytometric analysis were performed as previously described (23), using saturating concentrations of antibody.…”

Section: Methodsmentioning

confidence: 99%

Overexpression of CD70 and overstimulation of IgG synthesis by lupus T cells and T cells treated with DNA methylation inhibitors

Oelke¹,

Lu²,

Richardson³

et al. 2004

Arthritis & Rheumatism

220

182

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Objective. Generalized DNA hypomethylation contributes to altered T cell function and gene expression in systemic lupus erythematosus (SLE). Some of the overexpressed genes participate in the disease process, but the full repertoire of genes affected is unknown. Methylation-sensitive T cell genes were identified by treating T cells with the DNA methyltransferase inhibitor 5-azacytidine and comparing gene expression with oligonucleotide arrays. CD70, a costimulatory ligand for B cell CD27, was one gene that reproducibly increased. We then determined whether CD70 is overexpressed on T cells treated with other DNA methylation inhibitors and on SLE T cells, and determined its functional significance.Methods. Oligonucleotide arrays, real-time reverse transcription-polymerase chain reaction, and flow cytometry were used to compare CD70 expression in T cells treated with 2 DNA methyltransferase inhibitors (5-azacytidine and procainamide) and 3 ERK pathway inhibitors known to decrease DNA methyltransferase expression (U0126, PD98059, and hydralazine). The consequences of CD70 overexpression were tested by coculture of autologous T and B cells with and without anti-CD70 and measuring IgG production by enzymelinked immunosorbent assay. The results were compared with those of T cells from lupus patients. Results. SLE T cells and T cells treated with

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Age-related changes in mature CD4+ T cells: cell cycle analysis

Cited by 13 publications

References 32 publications

Age and Vitamin E-Induced Changes in Gene Expression Profiles of T Cells

Age and Vitamin E-Induced Changes in Gene Expression Profiles of T Cells

Effect of the Lycium barbarum polysaccharides on age-related oxidative stress in aged mice

Overexpression of CD70 and overstimulation of IgG synthesis by lupus T cells and T cells treated with DNA methylation inhibitors

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