Age-related changes in physicochemical properties of mineral crystals are related to impaired mechanical function of cortical bone

Akkuş, Ozan; Adar, Fran; Schaffler, Mitchell B.

doi:10.1016/j.bone.2003.11.003

Cited by 400 publications

(353 citation statements)

References 60 publications

Supporting

Mentioning

318

Contrasting

Unclassified

Order By: Relevance

“…Other studies applying Raman spectroscopy to human or murine bones of varying age have found that carbonate substitution increased with increasing age. (28,29,32) Since increasing age generally decreases remodeling activity and turnover, these results are in accordance with ours. Carbonate-to-phosphate ratio has been suggested previously to be related to the size of the mineral crystals in the bone tissue.…”

Section: Discussionsupporting

confidence: 92%

See 1 more Smart Citation

Infrared spectroscopy indicates altered bone turnover and remodeling activity in renal osteodystrophy

Isaksson

Turunen

Rieppo

et al. 2010

Journal of Bone and Mineral Research

View full text Add to dashboard Cite

Renal osteodystrophy alters metabolic activity and remodeling rate of bone and also may lead to different bone composition. The objective of this study was to characterize the composition of bone in high-turnover renal osteodystrophy patients by means of Fourier transform infrared spectroscopic imaging (FTIRI). Iliac crest biopsies from healthy bone (n ¼ 11) and patients with renal osteodystrophy (ROD, n ¼ 11) were used in this study. The ROD samples were from patients with hyperparathyroid disease. By using FTIRI, phosphate-toamide I ratio (mineral-to-matrix ratio), carbonate-to-phosphate ratio, and carbonate-to-amide I ratio (turnover rate/remodeling activity), as well as the collagen cross-link ratio (collagen maturity), were quantified. Histomorphometric analyses were conducted for comparison. The ROD samples showed significantly lower carbonate-to-phosphate ( p < .01) and carbonate-to-amide I ( p < .001) ratios. The spatial variation across the trabeculae highlighted a significantly lower degree of mineralization ( p < .05) at the edges of the trabeculae in the ROD samples than in normal bone. Statistically significant linear correlations were found between histomorphometric parameters related to bone-remodeling activity and number of bone cells and FTIRI-calculated parameters based on carbonate-to-phosphate and carbonate-to-amide I ratios. Hence the results suggested that FTIRI parameters related to carbonate may be indicative of turnover and remodeling rate of bone. ß 2010 American Society for Bone and Mineral Research.KEY WORDS: BONE COMPOSITION; HISTOMORPHOMETRY; FOURIER TRANSFORM; INFRARED; RENAL OSTEODYSTROPHY; CARBONATE B one is a composite material consisting of a mineral phase (hydroxyapatite), an organic phase (collagen), noncollagenous proteins, lipids, and water. (1,2) Metabolic bone disease is a common term referring to a number of abnormalities caused by a broad spectrum of disorders. Most disorders lead to weakening of the bone or impaired bone function and are followed most often by altered bone mechanical properties. Renal osteodystrophy (ROD) is a chronic kidney disease that can be accompanied by different types of bone pathologies. ROD can lead to defective mineralization, altered bone morphology, and/or bone turnover. (3,4) Commonly, it is accompanied by hyperparathyroid disease, which is characterized by increased bone turnover. Patients with renal osteodystrophy may exhibit bone and joint pain, bone deformation, and spontaneous bone fractures. Bone biopsies followed by quantitative histomorphometry prevail as the ''gold standard'' for the diagnosis of renal osteodystrophy. (5)(6)(7) Histomorphometric analysis emphasizes increased amounts of osteoid and erosion surface and a larger amount of bone cells than what is present in normal bone. (8) However, the invasive nature of obtaining the biopsies, the required experience of the pathologists to identify the abnormalities, as well as the lack of standardized normal limits remain problematic.ROD also commonly results in lower bone ...

show abstract

Section: Discussionsupporting

confidence: 92%

“…therefore would increase with increasing tissue age. (28)(29)(30)(31)(32) Since increasing tissue age is related to decreased bone turnover, the latter argument is supported by our data. However, the idea that the carbonate content is related to tissue age is not new.…”

Section: Discussionsupporting

confidence: 87%

Infrared spectroscopy indicates altered bone turnover and remodeling activity in renal osteodystrophy

Isaksson

Turunen

Rieppo

et al. 2010

Journal of Bone and Mineral Research

View full text Add to dashboard Cite

show abstract

“…This is consistent with [66], who found carbonate incorporation remained constant after 45 years old. When age matched, a mean difference of 0.002 ± 0.0004 was observed for the carbonate to phosphate ratios between the two groups.…”

Section: Ftirsupporting

confidence: 92%

Towards new material biomarkers for fracture risk

Greenwood

Clement

Dicken

et al. 2016

Bone

View full text Add to dashboard Cite

Osteoporosis is a prevalent bone condition, characterised by low bone mass and increased fracture risk. Currently, the gold standard for identifying osteoporosis and increased fracture risk is through quantification of bone mineral density (BMD) using dual energy X-ray absorption (DEXA). However, the risk of osteoporotic fracture is determined collectively by bone mass, architecture and physicochemistry of the mineral composite building blocks. Thus DEXA scans alone inevitably fail to fully discriminate individuals who will suffer a fragility fracture. This study examines trabecular bone at both ultrastructure and microarchitectural levels to provide a detailed material view of bone, and therefore provides a more comprehensive explanation of osteoporotic fracture risk. Physicochemical characterisation obtained through X-ray diffraction and infrared analysis indicated significant differences in apatite crystal chemistry and nanostructure between fracture and non-fracture groups.Further, this study, through considering the potential correlations between the chemical biomarkers and microarchitectural properties of trabecular bone, has investigated the relationship between bone mechanical properties (e.g. fragility) and physicochemical material features.

show abstract

“…4). It has been reported (23,24) that the spectroscopically determined carbonate content expressed as carbonate/phosphate ratio increases with specimen and tissue age; however, because the present data were acquired at locations of similar tissue age, the implication would be that this was due to qualitative differences in mineral crystallite chemical composition between the ZOL and placebo groups. Moreover, because of the anatomic area selection criteria employed in this study, these differences are not attributable to the antiresorptive effect of ZOL.…”

Section: Discussionmentioning

confidence: 70%

Bone material properties in actively bone-forming trabeculae in postmenopausal women with osteoporosis after three years of treatment with once-yearly Zoledronic acid

Gamsjaeger

Buchinger

Zwettler

et al. 2010

Journal of Bone and Mineral Research

View full text Add to dashboard Cite

Zoledronic acid (ZOL), a third-generation aminobisphosphonate, showed pronounced antifracture efficacy in a phase III clinical trial [Health Outcomes and Reduced Incidence with Zoledronic Acid Once Yearly-Pivotal Fracture Trial (HORIZON-PFT)] when administered yearly (5-mg infusions of ZOL), producing significant reductions in morphometric vertebral, clinical vertebral, hip, and nonvertebral fractures by 70%, 77%, 41%, and 25%, respectively, over a 3-year period. The purpose of this study was to analyze the biopsies obtained during the HORIZON clinical trial (152 patients, 82 ZOL and 70 placebo) by means of Raman microspectroscopy (a vibrational spectroscopic technique capable of analyzing undecalcified bone tissue with a spatial resolution of approximately 0.6 mm) to determine the effect of ZOL therapy on bone material properties (in particular mineral/matrix ratio, lamellar organization, carbonate and proteoglycan (based on spectral identification of glycosaminoglycan) content, and mineral maturity/crystallinity) at similar tissue age (based on the presence of tetracycline double labels). The results indicated that while ZOL administration increased the mineral/ matrix ratio compared with placebo, it also resulted in mineral crystallites with a quality profile (based on carbonate content and maturity/crystallinity characteristics) of younger (with respect to tissue age) bone. Since the comparisons between ZOL-and placebotreated patients were performed at similar tissue age at actively forming bone surfaces, these results suggest that ZOL may be exerting an effect on bone matrix formation in addition to its well-established antiresorptive effect, thereby contributing to its antifracture efficacy. ß

show abstract

Age-related changes in physicochemical properties of mineral crystals are related to impaired mechanical function of cortical bone

Cited by 400 publications

References 60 publications

Infrared spectroscopy indicates altered bone turnover and remodeling activity in renal osteodystrophy

Infrared spectroscopy indicates altered bone turnover and remodeling activity in renal osteodystrophy

Towards new material biomarkers for fracture risk

Bone material properties in actively bone-forming trabeculae in postmenopausal women with osteoporosis after three years of treatment with once-yearly Zoledronic acid

Contact Info

Product

Resources

About