Age‐related changes of NADPH‐diaphorase‐positive neurons in the rat inferior colliculus and auditory cortex

Sánchez‐Zuriaga, Daniel; Martí-Gutiérrez, Nuria; Cruz, Maria Ángeles Pérez De La; Peris-Sanchis, Maria Rosa

doi:10.1002/jemt.20512

Cited by 11 publications

(11 citation statements)

References 41 publications

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“…In previous studies, a significant increase in NADPH-d-positive neurons was reported in the superior olivary nucleus in aged hamsters [37] and rats [40]. Sánchez-Zuriaga et al showed evidence of a decreased area of NADPH-d-positive neurons in the dorsal cortex (DC) of the IC and an age-related loss of NADPH-d-positive neurons in the IC and primary cortical auditory area (Te1) in rats [41]. These changes were related to hearing impairments associated with increasing age.…”

Section: Regional Changes In Nadph-d In the Aging Brainmentioning

confidence: 99%

Alterations in Nitric Oxide Synthase in the Aged CNS

Jung

Huh

2012

Oxidative Medicine and Cellular Longevity

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Aging is associated with neuronal loss, gross weight reduction of the brain, and glial proliferation in the cortex, all of which lead to functional changes in the brain. It is known that oxidative stress is a critical factor in the pathogenesis of aging; additionally, growing evidence suggests that excessive nitric oxide (NO) production contributes to the aging process. However, it is still unclear how NO plays a role in the aging process. This paper describes age-related changes in the activity of NADPH-diaphorase (NADPH-d), a marker for neurons containing nitric oxide synthase (NOS), in many CNS regions. Understanding these changes may provide a novel perspective in identifying the aging mechanism.

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Section: Regional Changes In Nadph-d In the Aging Brainmentioning

confidence: 99%

Alterations in Nitric Oxide Synthase in the Aged CNS

Jung

Huh

2012

Oxidative Medicine and Cellular Longevity

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“…For example, the efficacy of GABAergic and glycinergic inhibitory mechanisms declines with age throughout the central auditory system (Caspary et al, , , , , ), and physiological studies in aged animals reveal varying degrees of degradation in the sound‐processing abilities of aged neurons (Mendelson and Rickets, ; Palombi et al, ; Lee et al, ). Interestingly, the expression of calcium‐binding proteins and nitric oxide synthases has also repeatedly been shown to change within the aging auditory system (O'Neill et al, ; Zettel et al, ; Ouda et al, , ; Sanchez‐Zuriaga et al, ; Huh et al, ). The vast majority of this work has been done in nonprimate models; however, physiological studies in primate auditory cortex suggest an analogous age‐related release from inhibition and a broadening in the spatial tuning of neurons (Juarez‐Salinas et al, ; Engle and Recanzone, ).…”

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confidence: 99%

“…NO also provides regulatory feedback to the NMDA receptor, along with its numerous signaling and modulatory actions (Hopper et al, ). Regardless of the exact purpose of NO in the auditory system, its relative expression consistently changes with age in rodents (Reuss et al, ; Ouda et al, ; Sanchez‐Zuriaga et al, ; Huh et al, ), and, as with PV, primate studies exploring these changes are sparse (Udell et al, ).…”

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confidence: 99%

Age‐related neurochemical changes in the rhesus macaque superior olivary complex

Gray

Engle

Recanzone

2013

J of Comparative Neurology

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Positive immunoreactivity to the calcium-binding protein parvalbumin (PV) and nitric oxide synthase NADPH-diaphorase (NADPHd) is well documented within neurons of the central auditory system of both rodents and primates. These proteins are thought to play roles in the regulation of auditory processing. Studies examining the age-related changes in expression of these proteins have been conducted primarily in rodents but are sparse in primate models. In the brainstem, the superior olivary complex (SOC) is crucial for the computation of sound source localization in azimuth, and one hallmark of age-related hearing deficits is a reduced ability to localize sounds. To investigate how these histochemical markers change as a function of age and hearing loss, we studied eight rhesus macaques ranging in age from 12 to 35 years. Auditory brainstem responses (ABRs) were obtained in anesthetized animals for click and tone stimuli. The brainstems of these same animals were then stained for PV and NADPHd reactivity. Reactive neurons in the three nuclei of the SOC were counted, and the densities of each cell type were calculated. We found that PV and NADPHd expression increased with both age and ABR thresholds in the medial superior olive but not in either the medial nucleus of the trapezoid body or the lateral superior olive. Together these results suggest that the changes in protein expression employed by the SOC may compensate for the loss of efficacy of auditory sensitivity in the aged primate.

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“…The parcelation of auditory structures based upon similar organizational characteristics was recently described in rodents and carnivores with cytochrome oxidase and NADPHd (Loftus et al, 2008). In these parallel pathways, the relative density of neurons that express either PV and/or NADPHd have been shown to change with age in rodents and non-human primates (Zettel et al, 1997; Reuss et al, 2000; Ouda et al, 2003, 2008; Sanchez-Zuriaga et al, 2007; Huh et al, 2008; Gray et al, 2013, 2014a,b), and are thought to play a compensatory role in response to decreased output from the cochlea and/or to changes in neuronal excitability (Gray et al, 2014b). …”

Section: Introductionmentioning

confidence: 99%

“…NADPHd has also been shown to co-localize with distinct calcium binding proteins (Yan et al, 1996), and shows age-related changes throughout the auditory system. Age-related changes in the number of the NADPHd positive cells have been found in the cochlear nucleus of macaques (Gray et al, 2014b), the superior olivary complex of macaques (Gray et al, 2013) and chinchillas (Reuss et al, 2000), as well as the inferior colliculus and auditory cortex of rats (Ouda et al, 2003; Sanchez-Zuriaga et al, 2007; Huh et al, 2008). …”

Section: Introductionmentioning

confidence: 99%

Age-related neurochemical changes in the rhesus macaque inferior colliculus

Engle

Gray

Turner

et al. 2014

Front. Aging Neurosci.

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Age-related hearing loss (ARHL) is marked by audiometric hearing deficits that propagate along the auditory pathway. Neurochemical changes as a function of aging have also been identified in neurons along the auditory pathway in both rodents and carnivores, however, very little is known about how these neurochemicals change in the non-human primate. To examine how these compensatory neurochemical changes relate to normal aging and audiometric sensitivity along the auditory pathway, we collected auditory brainstem responses (ABRs) and brain specimens from seven rhesus monkeys spanning in age from 15 to 35 years old, and examined the relationship between click evoked ABR thresholds and the ABR evoked pure tone average (PTA) and changes in the number of parvalbumin and NADPH-diaphorase positive cells in the auditory midbrain. We found that the number of parvalbumin positive cells in the central nucleus and the surrounding cortex regions of the inferior colliculus were strongly correlated with advancing age and ABR PTA. We also found that the numbers of NADPHd positive cells in these same regions were not associated with normal aging or changes in the ABR thresholds. These findings suggest that the auditory midbrain undergoes an up-regulation of parvalbumin expressing neurons with aging that is related to changes in the processing of frequencies across the audiometric range.

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Age‐related changes of NADPH‐diaphorase‐positive neurons in the rat inferior colliculus and auditory cortex

Cited by 11 publications

References 41 publications

Alterations in Nitric Oxide Synthase in the Aged CNS

Alterations in Nitric Oxide Synthase in the Aged CNS

Age‐related neurochemical changes in the rhesus macaque superior olivary complex

Age-related neurochemical changes in the rhesus macaque inferior colliculus

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