Age-related NADPH-diaphorase positive bodies in the lumbosacral spinal cord of aged rats

Tan, Huibing; He, J.; Wang, Songyan; Hirata, Ken‐ichi; Yang, Zhengwei; Kuraoka, Akio; Kawabuchi, Masaru

doi:10.1679/aohc.69.297

Cited by 19 publications

(41 citation statements)

References 36 publications

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“…Our hypothesis pointed out that N-d alterations in the GR (Ma et al, 1997) was correlated to ANB in the sacral segment (Tan et al, 2006) of the aging rats. We also found another kind of N-d neurodegeneration, megaloneurites (N-d meganeurites) in aged dogs (Li et al, 2018).…”

Section: Discussionmentioning

confidence: 77%

Dual forms of aging-related NADPH diaphorase neurodegeneration in the sacral spinal cord of aged non-human primates

Wei

Jia

et al. 2019

Preprint

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NADPH diaphorase (N-d) positive neurons has been examined in many animals. N-d neurodegenerative neurites were detected in some animal models. However, detailed information of N-d positivity and aging related changes was still lack in the spinal cord and medulla oblongata of pigeons. In this study, we evaluated the N-d positivity and aging alterations in the spinal cord and medullary oblongata of the pigeon compared with rat and mouse. In pigeons, N-d neurons were more numerous in the dorsal horn, around the central canal and in the column of Terni in the thoracic and lumbar segments and scattered neurons occurred in the ventral horn of spinal segments. N-d neurons also occurred in the white matter of spinal cord. Morphometrical analysis demonstrated in the lumbosacral, cervical and thoracic regions. Compared with young pigeons, the size of N-d soma was significantly altered in aged pigeons. Meanwhile, the dramatic morphological changes occurred in the lumbar to sacral segments. The most important findings of this study were aging-related N-d positive bodies (ANB) in aged pigeons, mainly in the nucleus cuneatus externus (CuE), occasionally in the nuclei gracilis et cuneatus. ANBs were identified in the gracile nuclei in spinal cord in the aged rats and mice. ANBs also detected in the CuE spinal nucleus in the aged rats. Immunohistochemistry also showed that the aging changes occurred in the cell types and neuropeptides in aged animals. The results suggested the weak inflammation and neuronal dysfunction in the spinal cord in aged pigeons. Our results suggested that the ANB could be considered as aging marker in the central nervous system.

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Section: Discussionmentioning

confidence: 77%

Dual forms of aging-related NADPH diaphorase neurodegeneration in the sacral spinal cord of aged non-human primates

Wei

Jia

et al. 2019

Preprint

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“…Tan et al found that NADPH-d-positive neurons are present in the lumbosacral spinal cords of aged rats. However, no colocalization of NADPH-d-positive and n NOS-IR neurons was detected in the lumbosacral spinal cords of aged rats [57]. Thus, NADPH-d activity does not always coincide with the NO-containing neural structures, and NADPH-d function in spinal cord is also unclear.…”

Section: Regional Changes In Nadph-d In the Aging Brainmentioning

confidence: 99%

Alterations in Nitric Oxide Synthase in the Aged CNS

Jung

Huh

2012

Oxidative Medicine and Cellular Longevity

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Aging is associated with neuronal loss, gross weight reduction of the brain, and glial proliferation in the cortex, all of which lead to functional changes in the brain. It is known that oxidative stress is a critical factor in the pathogenesis of aging; additionally, growing evidence suggests that excessive nitric oxide (NO) production contributes to the aging process. However, it is still unclear how NO plays a role in the aging process. This paper describes age-related changes in the activity of NADPH-diaphorase (NADPH-d), a marker for neurons containing nitric oxide synthase (NOS), in many CNS regions. Understanding these changes may provide a novel perspective in identifying the aging mechanism.

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“…The nicotinamide adenine dinucleotide phosphate-diaphorase (NADPH-d) reaction is used as a marker to characterize certain neuronal properties and colocalize with nitric oxide synthase (NOS) (Dawson et al, 1991;Hope et al, 1991). However, some researchers demonstrate that NADPH-d is not always identical to NOS (Belai et al, 1992;Traub et al, 1994;Pullen and Humphreys, 1995;Tan et al, 2006). In both central and peripheral nervous system, only a part of NOS positive neurons colocalize with NADPH-d histochmistry (Belai et al, 1992).…”

Section: Introductionmentioning

confidence: 99%

De novo aging-related megaloneurites: alteration of NADPH diaphorase positivity in the sacral spinal cord of the aged dog

Jia

Hou

et al. 2018

Preprint

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The aging-related changes of NADPH-diaphorase (NADPH-d) in the spinal cord were studied in aged dogs. At all levels of the spinal cord examined, NADPH-d activities were present in neurons and fibers in the superficial dorsal horn, dorsal commissure and in neurons around the central canal. In addition, the sympathetic autonomic nucleus in the thoracic and rostral lumbar segments exhibited prominent NADPH-d cellular staining whereas the sacral parasympathetic nucleus (SPN) in the sacral segments was not well stained. Interestingly, we found abundant NADPH-d positive enlarged-diameter fibers termed megaloneurite, which characteristically occurred in the aged sacral segments, distributed in the dorsal gray commissure (DGC), lateral collateral pathway (LCP) the lateral fasciculi and the central canal compared with the cervical, thoracic and lumbar segments. The dense, abnormal NADPH-d megaloneurites occurred in extending from dorsal entry zone through lamina I along with the lateral boundary of the dorsal horn to the region of the SPN. These fibers were prominent in the S1-S3 segments but not in adjacent segments L5-L7 and Cx1 or in thoracolumbar segments and cervical segments. Double staining with GFAP, NeuN, CGRP, MAP2 and Iba1, NADPH-d megaloneurite colocalized with vasoactive intestinal peptide. Presumably, the megaloneurites may represent, in part, visceral afferent projections to the SPN and/or DGC. The NADPH-d megaloneurites in the aged sacral spinal cord indicated some anomalous changes in the neurites, which might account for a disturbance in the aging pathway of the autonomic and sensory nerve in the pelvic visceral organs.

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Age-related NADPH-diaphorase positive bodies in the lumbosacral spinal cord of aged rats

Cited by 19 publications

References 36 publications

Dual forms of aging-related NADPH diaphorase neurodegeneration in the sacral spinal cord of aged non-human primates

Dual forms of aging-related NADPH diaphorase neurodegeneration in the sacral spinal cord of aged non-human primates

Alterations in Nitric Oxide Synthase in the Aged CNS

De novo aging-related megaloneurites: alteration of NADPH diaphorase positivity in the sacral spinal cord of the aged dog

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