Age-related variations in follicular apolipoproteins may influence human oocyte maturation and fertility potential

Wald, Tiffany Von; Monisova, Y.; Hacker, Michele R.; Yoo, Seung‐Min; Penzias, Alan S.; Reindollar, Richard R.; Usheva, Anny

doi:10.1016/j.fertnstert.2008.12.129

Cited by 50 publications

(43 citation statements)

References 26 publications

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“…Thus, it is generally accepted that in mammals HDL is the sole lipoprotein present in follicular fluid due to the porosity of the follicle basement membrane which is permeable to serum proteins up to 300 kDa in size (Shalgi et al 1973), thus excluding LDL and VLDL. Interestingly, although LDL and/or VLDL are detected in follicular fluid of some women (Von Wald et al 2010) and it is also reported that human granulosa-lutein cells express lipoprotein marker ApoB-100 and assemble and secrete in vitro-native VLDL particles similar to those in serum, except with slightly higher triglyceride content and less cholesterol (Gautier et al 2010). Thus, LDL and VLDL particles detected in follicular fluid may in fact be generated by ovarian cells.…”

Section: Fatty Acid Supply To the Cocmentioning

confidence: 98%

Lipids and oocyte developmental competence: the role of fatty acids and β-oxidation

2014

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Metabolism and ATP levels within the oocyte and adjacent cumulus cells are associated with quality of oocyte and optimal development of a healthy embryo. Lipid metabolism provides a potent source of energy and its importance during oocyte maturation is being increasingly recognised. The triglyceride and fatty acid composition of ovarian follicular fluid has been characterised for many species and is influenced by nutritional status (i.e. dietary fat, fasting, obesity and season) as well as lactation in cows. Lipid in oocytes is a primarily triglyceride of specific fatty acids which differ by species, stored in distinct droplet organelles that re-localise during oocyte maturation. The presence of lipids, particularly saturated vs unsaturated fatty acids, in in vitro maturation systems affects oocyte lipid content as well as developmental competence. Triglycerides are metabolised by lipases that have been localised to cumulus cells as well as oocytes. Fatty acids generated by lipolysis are further metabolised by b-oxidation in mitochondria for the production of ATP. b-oxidation is induced in cumulus-oocyte complexes (COCs) by the LH surge, and pharmacological inhibition of b-oxidation impairs oocyte maturation and embryo development. Promoting b-oxidation with L-carnitine improves embryo development in many species. Thus, fatty acid metabolism in the mammalian COC is regulated by maternal physiological and in vitro environmental conditions; and is important for oocyte developmental competence.

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Section: Fatty Acid Supply To the Cocmentioning

confidence: 98%

Lipids and oocyte developmental competence: the role of fatty acids and β-oxidation

2014

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“…FF results from the transfer of blood plasma components and the secretory activity of the oocyte, granulosa and thecal cells. It contains over 200 different proteins that reflect the stage of oocyte development and the degree of follicle maturation [1,2,3]. Several studies explained the presence of gonadotropins and steroids in the FF as the markers for oocyte maturation [4,5,6].…”

Section: Introductionmentioning

confidence: 99%

“…This microenvironment may be altered by conditions, such as endometriosis [21,22,23], polycystic ovary syndrome [24] and reproductive aging [25,26,27]. Reproductive aging is marked by the decline in ovarian response, oocyte number [1,28,29,30,31] and quality [30]. Female age, quality and quantity of the retrieved oocytes are the major factors affecting in vitro fertilization (IVF) outcomes.…”

Section: Introductionmentioning

confidence: 99%

“…Some of the FF markers could be correlated to reproductive aging, such as interleukin-8 [20] GDF-9 and TGF-β1 [32], inhibin [33], anti-Müllerian hormone [10] and bone morphogenetic protein 15 [18]. Therefore, proteome analysis of FF might be helpful in evaluating oocyte quality and identifying predictive markers for oocyte developmental potential prior to fertilization and the success in assisted reproductive technology (ART) [1,20,34]. Two-dimensional electrophoresis (2-DE) was used by Spitzer et al (1996), for the first time, to compare protein patterns of mature and immature human follicles [35].…”

Section: Introductionmentioning

confidence: 99%

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A Proteomic Analysis of Human Follicular Fluid: Comparison between Younger and Older Women with Normal FSH Levels

Hashemitabar

Bahmanzadeh

Mostafaie

et al. 2014

IJMS

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The follicular fluid (FF) is produced during folliculogenesis and contains a variety of proteins that play important roles in follicle development and oocyte maturation. Age-related infertility is usually considered as a problem that can be solved by assisted reproduction technology. Therefore, the identiﬁcation of novel biomarkers that are linked to reproductive aging is the subject of this study. FF was obtained from healthy younger (20–32 years old) and older (38–42 years old) women undergoing intracytoplasmic sperm injection (ICSI) due to male factor infertility. The FF was analyzed by two-dimensional gel electrophoresis (2-DE). The power of two-dimensional gel electrophoresis and the identiﬁcation of proteins were exploited using matrix-assisted laser desorption-ionization time-of-flight/time-of-flight (MALDI-TOF-TOF) mass spectrometry. Twenty three protein spots showed reproducible and significant changes in the aged compared to the young group. Of these, 19 protein spots could be identified using MALDI-TOF-TOF-MS. As a result of MASCOT search, five unique downregulated proteins were identified in the older group. These were identified as serotransferrin, hemopexin precursor, complement C3, C4 and kininogen. A number of protein markers were found that may help develop diagnostic methods of infertility.

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“…However, Apoa1 null mice are fertile although the lipid and apolipoprotein compositions of HDL in Apoa1 null mice differ from those in wild type mice [59,60]. Recent studies have also indicated that Apoa1 may affect oocyte maturation and fertility potential [61]. In this regard, HDL receptor null females mice are infertile due to ovarian defects as a consequence of altered lipoprotein metabolism, but this is reversible [59].…”

mentioning

confidence: 99%

Progesterone Receptor-Induced Gene Expression in Primary Mouse Granulosa Cell Cultures1

Sriraman

Sinha

Richards

2010

Biology of Reproduction

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The progesterone receptor (PGR) is induced by luteinizing hormone (LH) in granulosa cells of preovulatory follicles, and the PGR-A isoform is essential for ovulation based on the phenotypes of Pgr isoform-specific knockout mice. Although several genes regulated by PGR-A in vivo have been identified, whether these genes are primary targets of PGR-A or if their expression also depends on other signaling molecules that are induced by the LH surge has not been resolved. Therefore, to identify genes that are either induced or repressed by PGR in the absence of LH-mediated signaling cascades, we infected primary cultures of mouse granulosa cells with either PGR-A or PGR-B adenoviral vectors without or with R-5020 as a PGR ligand. Total RNA was extracted from infected cells at 16 h and analyzed by Affymetrix Mouse 430 2.0 microarrays. PGR-A in the presence or absence of ligand significantly induced approximately 50 genes 2-fold or more (local pooled error test at P

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Age-related variations in follicular apolipoproteins may influence human oocyte maturation and fertility potential

Cited by 50 publications

References 26 publications

Lipids and oocyte developmental competence: the role of fatty acids and β-oxidation

Lipids and oocyte developmental competence: the role of fatty acids and β-oxidation

A Proteomic Analysis of Human Follicular Fluid: Comparison between Younger and Older Women with Normal FSH Levels

Progesterone Receptor-Induced Gene Expression in Primary Mouse Granulosa Cell Cultures1

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