Aging, Cardiac Hypertrophy and Ischemic Cardiomyopathy Do Not Affect the Proportion of Mononucleated and Multinucleated Myocytes in the Human Heart

Olivetti, G; Cigola, Elena; Maestri, Roberta; Corradi, Domenico; Lagrasta, Costanza; Gambert, Steven; Anversa, Piero

doi:10.1006/jmcc.1996.0137

Cited by 196 publications

(157 citation statements)

References 2 publications

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“…4B) and remained unchanged throughout life. The percentage of mononucleated cardiomyocytes in children has not been published previously, but the percentage in adults is in agreement with a prior study (24). In conclusion, humans do not display the transition to predominantly binucleated cardiomyocytes, which is known to happen in murine models in the first week after birth (12,25).…”

Section: Resultssupporting

confidence: 91%

“…Bland-Altman analysis (SI Appendix, Fig. S5 C and D) revealed a significant agreement between the model-based (24,25) and our design-based method for volume determination.…”

Section: Methodsmentioning

confidence: 64%

“…The mean of 60-100 isolated cardiomyocytes from each heart was calculated. We validated this design-based method using a model-based method that assumed a cylindrical shape of the cardiomyocyte (24,25). Bland-Altman analysis (SI Appendix, Fig.…”

Section: Methodsmentioning

confidence: 99%

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Cardiomyocyte proliferation contributes to heart growth in young humans

Mollova¹,

Bersell

Walsh³

et al. 2013

Proc. Natl. Acad. Sci. U.S.A.

647

578

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The human heart is believed to grow by enlargement but not proliferation of cardiomyocytes (heart muscle cells) during postnatal development. However, recent studies have shown that cardiomyocyte proliferation is a mechanism of cardiac growth and regeneration in animals. Combined with evidence for cardiomyocyte turnover in adult humans, this suggests that cardiomyocyte proliferation may play an unrecognized role during the period of developmental heart growth between birth and adolescence. We tested this hypothesis by examining the cellular growth mechanisms of the left ventricle on a set of healthy hearts from humans aged 0-59 y (n = 36). The percentages of cardiomyocytes in mitosis and cytokinesis were highest in infants, decreasing to low levels by 20 y. Although cardiomyocyte mitosis was detectable throughout life, cardiomyocyte cytokinesis was not evident after 20 y. Between the first year and 20 y of life, the number of cardiomyocytes in the left ventricle increased 3.4-fold, which was consistent with our predictions based on measured cardiomyocyte cell cycle activity. Our findings show that cardiomyocyte proliferation contributes to developmental heart growth in young humans. This suggests that children and adolescents may be able to regenerate myocardium, that abnormal cardiomyocyte proliferation may be involved in myocardial diseases that affect this population, and that these diseases might be treatable through stimulation of cardiomyocyte proliferation.heart failure | pediatrics H eart failure, a leading public health problem worldwide (1), is linked to the loss of cardiomyocytes (2-4). The only currently available, definitive therapy-heart transplantation-is limited by donor availability. New approaches, such as cell transplantation, have shown encouraging results in clinical trials (5, 6). However, a third, complementary strategy has emerged, based on stimulating endogenous regenerative mechanisms. One approach for developing such regeneration strategies is to examine the cellular mechanisms of myocardial growth, since mechanisms of regeneration should be similar to the mechanisms of development.Although stem and progenitor cells are important for morphogenesis of the myocardium, developmental growth in a number of nonhuman species is largely driven by cardiomyocyte proliferation (7-9). In biological models that, unlike adult humans, regenerate myocardium, cardiomyocyte proliferation is important for regeneration as well as postnatal heart growth (10, 11). For example, in mice, developmental cardiomyocyte proliferation continues for up to day 7 after birth, which coincides with the loss of regenerative capacity (11,12). The close temporal relationship between cardiomyocyte proliferation and heart regeneration in animals raises the question of whether and to what age and extent cardiomyocyte proliferation plays a role in humans. The answer may help us understand the endogenous regenerative potential of the human heart and possibly indicate strategies for stimulating cardiomyocyte proliferation to reg...

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Section: Resultssupporting

confidence: 91%

“…Bland-Altman analysis (SI Appendix, Fig. S5 C and D) revealed a significant agreement between the model-based (24,25) and our design-based method for volume determination.…”

Section: Methodsmentioning

confidence: 64%

See 1 more Smart Citation

Cardiomyocyte proliferation contributes to heart growth in young humans

Mollova¹,

Bersell

Walsh³

et al. 2013

Proc. Natl. Acad. Sci. U.S.A.

647

578

View full text Add to dashboard Cite

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“…A tentativa para estabelecer parâmetros quantitativos no coração durante o envelhecimento é de importância médica e biológica 1,[5][6][7][8][9][10][11][12] .…”

Section: Estereologia Do Miocárdio De Ratos Jovens E Idosos Artigo Orunclassified

Estereologia do miocárdio de ratos jovens e idosos

Águila¹,

Mandarim-de-Lacerda²,

Apfel³

1998

Arq. Bras. Cardiol.

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Purpose - Estereologia do Miocárdio de Ratos Jovens e Idosos Artigo OriginalAs mudanças estruturais do miocárdio decorrente do envelhecimento ainda não estão totalmente esclarecidas na literatura médica. Sabe-se que, com o avançar da idade, há diminuição do número e hipertrofia dos miócitos cardíacos. A estereologia é um método de morfologia quantitativa que permite determinar com precisão estas alterações do miocárdio na idade avançada 1 .

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“…[36][37][38] Evidence suggests that there is an increase in left ventricular mass with aging, in which heart weight can increase 1 g to 1.5 g per year. [39,40] Although, studies that exclude individuals with heart diseases fail to report significant changes in left ventricular mass.…”

Section: Changes In Ventricle Structurementioning

confidence: 99%

Mitochondria and the Aging Heart

Ketul¹,

Chaudhary²,

Haitham³

et al. 2005

Mitochondria and the Heart

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The average human life span has markedly increased in modern society largely attributed to advances in medical and therapeutic sciences that have successfully reduced important health risks. However, advanced age results in numerous alterations to cellular and subcellular components that can impact the overall health and function of an individual. Not surprisingly, advanced age is a major risk factor for the development of heart disease in which elderly populations observe increased morbidity and mortality. Even healthy individuals that appear to have normal heart function under resting conditions, actually have an increased susceptibility and vulnerability to stress. This is confounded by the impact that stress and disease can have over time to both the heart and vessels. Although, there is a rapidly growing body of literature investigating the effects of aging on the heart and how age-related alterations affect cardiac function, the biology of aging and underlying mechanisms remain unclear. In this review, we summarize effects of aging on the heart and discuss potential theories of cellular aging with special emphasis on mitochondrial dysfunction.

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Aging, Cardiac Hypertrophy and Ischemic Cardiomyopathy Do Not Affect the Proportion of Mononucleated and Multinucleated Myocytes in the Human Heart

Cited by 196 publications

References 2 publications

Cardiomyocyte proliferation contributes to heart growth in young humans

Cardiomyocyte proliferation contributes to heart growth in young humans

Estereologia do miocárdio de ratos jovens e idosos

Mitochondria and the Aging Heart

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