Agitation Induced Mechanical Stress in Stirred Tank Bioreactors—Linking CFD Simulations to Fungal Morphology

Esfandabadi, Manely Eslahpazir; Wucherpfennig, Thomas; Krull, Rainer

doi:10.1252/jcej.12we019

Cited by 6 publications

(2 citation statements)

References 19 publications

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“…In STB, filamentous microorganisms are subjected to turbulent stress due to the power input of the stirrer and aeration ( Lin et al, 2010 ). CFD simulations of a STB showed that the normal Reynolds stresses were significantly greater than the shear Reynolds stresses ( Eslahpazir Esfandabadi, 2013 ). The authors explained the observed reduction in pellet size by suggesting that the pellets were first deformed by normal forces and then damaged by erosion due to shear forces ( Eslahpazir Esfandabadi, 2013 ).…”

Section: Resultsmentioning

confidence: 99%

“…CFD simulations of a STB showed that the normal Reynolds stresses were significantly greater than the shear Reynolds stresses ( Eslahpazir Esfandabadi, 2013 ). The authors explained the observed reduction in pellet size by suggesting that the pellets were first deformed by normal forces and then damaged by erosion due to shear forces ( Eslahpazir Esfandabadi, 2013 ). The beads in the ShF induce shear stress

and normal stress

in the contact zone.…”

Section: Resultsmentioning

confidence: 99%

See 1 more Smart Citation

Quantification and modeling of macroparticle-induced mechanical stress for varying shake flask cultivation conditions

Schrader,

Schrinner,

Polomsky

et al. 2023

Front. Bioeng. Biotechnol.

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In biotechnological processes, filamentous microorganisms are known for their broad product spectrum and complex cellular morphology. Product formation and cellular morphology are often closely linked, requiring a well-defined level of mechanical stress to achieve high product concentrations. Macroparticles were added to shake flask cultures of the filamentous actinomycete Lentzea aerocolonigenes to find these optimal cultivation conditions. However, there is currently no model concept for the dependence of the strength and frequency of the bead-induced stress on the process parameters. Therefore, shake flask simulations were performed for combinations of bead size, bead concentration, bead density and shaking frequency. Contact analysis showed that the highest shear stresses were caused by bead-bottom contacts. Based on this, a newly generated characteristic parameter, the stress area ratio (SAR), was defined, which relates the bead wall shear and normal stresses to the total shear area. Comparison of the SAR with previous cultivation results revealed an optimum pattern for product concentration and mean product-to-biomass related yield coefficient. Thus, this model is a suitable tool for future optimization, comparison and scaling up of shear-sensitive microorganism cultivation. Finally, the simulation results were validated using high-speed recordings of the bead motion on the bottom of the shake flask.

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Section: Resultsmentioning

confidence: 99%

and normal stress

in the contact zone.…”

Section: Resultsmentioning

confidence: 99%

Quantification and modeling of macroparticle-induced mechanical stress for varying shake flask cultivation conditions

Schrader,

Schrinner,

Polomsky

et al. 2023

Front. Bioeng. Biotechnol.

Self Cite

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show abstract

Modeling, Simulation and Experimental Characterization of Mechanical and Surface-Induced Stresses on Pellets of the Filamentous Microorganism Lentzea aerocolonigenes

Dinius,

Schrader,

Schrinner

et al. 2024

Dispersity, Structure and Phase Changes of Proteins and Bio Agglomerates in Biotechnological Processes

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Viability characterization of Taxus chinensis plant cell suspension cultures by rapid colorimetric- and image analysis-based techniques

Wucherpfennig

Schulz

Pimentel

et al. 2014

Bioprocess Biosyst Eng

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For the commercially established process of paclitaxel production with Taxus chinensis plant cell culture, the size of plant cell aggregates and phenotypic changes in coloration during cultivation have long been acknowledged as intangible parameters. So far, the variability of aggregates and coloration of cells are challenging parameters for any viability assay. The aim of this study was to investigate simple and non-toxic methods for viability determination of Taxus cultures in order to provide a practicable, rapid, robust and reproducible way to sample large amounts of material. A further goal was to examine whether Taxus aggregate cell coloration is related to general cell viability and might be exploited by microscopy and image analysis to gain easy access to general cell viability. The Alamar Blue assay was found to be exceptionally eligible for viability estimation. Moreover, aggregate coloration, as a morphologic attribute, was quantified by image analysis and found to be a good and traceable indicator of T. chinensis viability.

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Agitation Induced Mechanical Stress in Stirred Tank Bioreactors—Linking CFD Simulations to Fungal Morphology

Cited by 6 publications

References 19 publications

Quantification and modeling of macroparticle-induced mechanical stress for varying shake flask cultivation conditions

Quantification and modeling of macroparticle-induced mechanical stress for varying shake flask cultivation conditions

Modeling, Simulation and Experimental Characterization of Mechanical and Surface-Induced Stresses on Pellets of the Filamentous Microorganism Lentzea aerocolonigenes

Viability characterization of Taxus chinensis plant cell suspension cultures by rapid colorimetric- and image analysis-based techniques

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