AglnS2/In2S3 heterostructure sensitization of Escherichia coli for sustainable hydrogen production

Abstract

Solar-to-chemical production by photosynthetic biohybrid systems does not only take advantage of the broadband light efficiency of semiconductor but also utilize highly specific biological catalytic power in living organism. Herein, we demonstrate a tandem inorganic-biological hybrid by combining AglnS 2 /In 2 S 3 and a facultative anaerobic bacterium, Escherichia coli, for biological H 2 production. The AglnS 2 /In 2 S 3 @E. coli hybrid system harvests light energy and makes use of anaerobically synthesized bacterial endogenous [Ni-Fe]-hydrogenase and photo-generated electrons from AglnS 2 /In 2 S 3 hybrid for enhanced H 2 evolution efficiency. A highly quantum efficiency (QE) of 3.3% at 720 nm for H 2 production is achieved from the hybrid system, exceeding those of many reported photoheterotrophic bacteria. This biomimetic approach may provide a guidance for the interfacing of hybrid semiconductors with living organisms for solar-to-chemical production.

Highly efficient charge transfer in CdS-covalent organic framework nanocomposites for stable photocatalytic hydrogen evolution under visible light

“…For the high-resolution S 2p spectrum, two peaks located at 162.42 and 161.19 eV are observed, revealing the existence of S 2- (Fig. 2d) [49,50]. These results adequately support the existence of CdS in 20%CdS-CTF-1.…”

Photo‐biohydrogen Production by Photosensitization with Biologically Precipitated Cadmium Sulfide in Hydrogen‐Forming Recombinant Escherichia coli

“…Compared with other E. coli whole-cell-based systems sensitized with bacterially precipitated or externally added inorganic semiconductors for photocatalytic hydrogen production, [12,13,21] including our previous system, [8b] the AQY for photocatalytic hydrogen production of our system seems to be low at present (Table S3). In E. coli system with CdS providing the data of quantum efficiencies, for example, the quantum yield at 470 and 620 nm for hydrogen formation were reported to be 7.93 and 9.59 %, respectively.…”

“…[12] In addition to this system, in non-genetically modified E. coli, bacterially precipitated CdS or other metal sulfides can alter the metabolites during glucose metabolism to increase fermentative hydrogen formation under light irradiation. [13] In the present study, motivated by these E. coli whole-cellbased studies, [12,13] we employed in situ bacterial CdS precipitation to construct a new photocatalytic hydrogen production system based on whole-cell recombinant E. coli expressing clostridial [FeFe]-hydrogenase genes (Figure 1). With these findings, we aim to expand current knowledge of photosensitization using a whole-cell biocatalyst with bacterially precipitated semiconductor materials.…”

Material–Microbe Interfaces for Solar-Driven CO2 Bioelectrosynthesis

“…[56]. In the same year, a heterojunction light harvester composed of AglnS 2 /In 2 S 3 on Escherichia coli and its application for H 2 generation was investigated [57]. Ji and colleagues reported a strategy to wrap M. thermoacetica in a metal-organic framework (MOF) monolayer [58].…”

“…Such hybrid systems can be used as synthetic biological tools where the direct activation of the enzyme by light can trigger the synthesis of fuels and chemicals from CO 2 with a higher QE ( Figure 3D). Multiple core-shell NPs with a zinc outer layer, including CdS@ZnS, CdSe@ZnS, InP@ZnS, and [57], and indium phosphide [37].…”