AgNOR Pleomorphic Count as a Tumor Marker in Cervical Carcinogenesis and Feasibility of Its Introduction in Cervical Cancer Screening Programs to Discriminate High-Risk Cases of Squamous Intraepithelial Lesions of the Cervix

Abstract

Background: AgNOR pleomorphism has been widely used for its diagnostic importance in differentiating premalignant and malignant lesions of different human neoplasms. However, an evaluation of its potential for discriminating cases of high-risk squamous intraepithelial lesions of the cervix (SIL) has been rarely attempted. Aim: The tumor marker potential of AgNOR pleomorphism counts was assessed by correlating high and low mean counts in low-grade SIL (LSIL) cases with persistence or regression of the lesion and HPV positivity. Materials and Methods: The 115 LSIL cases selected for the study were registered from the ongoing cervical cancer screening of the rural population of Lucknow West. Silver nitrate staining for AgNOR counts and HPV DNA testing were done in all 115 cases. Results: The AgNOR counts in the 115 LSIL cases revealed low counts in 92 and high counts in 23 cases. Follow-up, available in 107 cases, revealed persistence of the lesion in 21 of the 23 cases with high counts and in 4 of the 84 cases with low counts. HPV positivity showed a strong correlation with high counts. Persistence of LSIL was also more frequent with high AgNOR counts and in HPV-positive cases. Conclusions: The study showed a correlation of high mean AgNOR counts with HPV positivity and persistence of LSIL.

Argyrophilic nucleolar organizer regions staining for cytology smears in dogs and cats

“…The AgNOR stain can be applied for cytological smears and may be a useful cytological tool to predict the prognosis of malignant tumors in both humans [ 5 , 6 , 10 , 14 ] and dogs [ 1 , 7 , 8 , 15 , 16 ]. The AgNORs in tumor cells were assessed without counterstaining in the previous studies; however, in the cytology of dogs and cats, it is often difficult to accurately interpret the results of AgNOR staining because the tumor and non-tumor cells, such as inflammatory cells, are mixed in a disorderly fashion on the smear.…”

The investigation of the antitumoral effect of Cornus mas L in mice with ehrlich solid tumor

“…It is important to identify new biomarkers to differentiate benign and malignant lesions. In addition, the selection of the most reliable therapeutic strategy for cancer treatment is very important for the management of the treatment strategy and to improve success (16,18,36). We aimed to determine the average number of AgNOR in our study.…”