Agricultural potential of rhizospheric Bacillus subtilis strains exhibiting varied efficiency of surfactin production

Paraszkiewicz, Katarzyna; Bernat, Przemysław; Siewiera, Paulina; Moryl, Magdalena; Sas-Paszt, Lidia; Trzciński, Paweł; Jałowiecki, Łukasz; Płaza, Grażyna

doi:10.1016/j.scienta.2017.07.034

Cited by 30 publications

(15 citation statements)

References 37 publications

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“…Indeed, the first cluster of peaks near 1000 m/z are attributed to the lipopeptide homologues of surfactins and iturin family, while the second cluster of peaks near 1400 m/z is attributed to the lipopeptide homologues of fengycin family. In agreement with the result of this study, previous investigations reported that these nonribosomally synthesized CLPs produced by Bacillus have been found to be active against a wide range of phytopathogens (Roongsawang et al 2002;Ongena and Jacques 2008;Arguelles-Arias et al 2009;Zeriouh et al 2011;Hoefler et al 2012;Baindara et al 2013;Guo et al 2014;Bernat et al 2016;Paraszkiewicz et al 2017;Sarwar et al 2018).…”

Section: Discussionsupporting

confidence: 92%

Halotolerant bacteria belonging to operational group Bacillus amyloliquefaciens in biocontrol of the rice brown stripe pathogen Acidovorax oryzae

et al. 2018

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Section: Discussionsupporting

confidence: 92%

Halotolerant bacteria belonging to operational group Bacillus amyloliquefaciens in biocontrol of the rice brown stripe pathogen Acidovorax oryzae

et al. 2018

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“…In addition, surfactins are thought to play a key role in triggering ISR [32]. They may also contribute to swarming motility [33, 34], which can help achieve effective rhizosphere colonization and facilitate plant growth promoting traits while simultaneously inhibiting competing microorganisms via antimicrobial activity [34].…”

Section: Discussionmentioning

confidence: 99%

Characterization and complete genome analysis of the surfactin-producing, plant-protecting bacterium Bacillus velezensis 9D-6

Grady

MacDonald

et al. 2019

BMC Microbiol

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BackgroundBacillus velezensis is an endospore-forming, free-living soil bacterium with potential as a biopesticide against a broad spectrum of microbial pathogens of plants. Its potential for commercial development is enhanced by rapid replication and resistance to adverse environmental conditions, typical of Bacillus species. However, the use of beneficial microbes against phytopathogens has not gained dominance due to limitations that may be overcome with new biopesticidal strains and/or new biological knowledge.ResultsHere, we isolated B. velezensis strain 9D-6 and showed that it inhibits the in vitro growth of prokaryotic and eukaryotic pathogens, including the bacteria Bacillus cereus, Clavibacter michiganensis, Pantoea agglomerans, Ralstonia solanacearum, Xanthomonas campestris, and Xanthomonas euvesicatoria; and the fungi Alternaria solani, Cochliobolus carbonum, Fusarium oxysporum, Fusarium solani, Gibberella pulicaris, Gibberella zeae, Monilinia fructicola, Pyrenochaeta terrestris and Rhizoctonia solani. Antimicrobial compounds with activity against Clavibacter michiganensis were isolated from B. velezensis 9D-6 and characterized by high resolution LC-MS/MS, yielding formulae of C52H91N7O13 and C53H93N7O13, which correspond to [Leu7] surfactins C14 and C15 (also called surfactin B and surfactin C), respectively. We further sequenced the B. velezensis 9D-6 genome which consists of a single circular chromosome and revealed 13 gene clusters expected to participate in antimicrobial metabolite production, including surfactin and two metabolites that have not typically been found in this species - ladderane and lantipeptide. Despite being unable to inhibit the growth of Pseudomonas syringae DC3000 in an in vitro plate assay, B. velezensis 9D-6 significantly reduced root colonization by DC3000, suggesting that 9D-6 uses methods other than antimicrobials to control phytopathogens in the environment. Finally, using in silico DNA-DNA hybridization (isDDH), we confirm previous findings that many strains currently classified as B. amyloliquefaciens are actually B. velezensis.ConclusionsThe data presented here suggest B. velezensis 9D-6 as a candidate plant growth promoting bacterium (PGPB) and biopesticide, which uses a unique complement of antimicrobials, as well as other mechanisms, to protect plants against phytopathogens. Our results may contribute to future utilization of this strain, and will contribute to a knowledge base that will help to advance the field of microbial biocontrol.Electronic supplementary materialThe online version of this article (10.1186/s12866-018-1380-8) contains supplementary material, which is available to authorized users.

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“…However, apart from a few biosurfactants such as sophorolipids, rhamnolipids, and mannosyl erythritol lipids (Chen, Juang, & Wei, ; Desai & Banat, ), their industrial application is largely restricted. Surfactin, a lipopeptide biosurfactant produced by Bacillus strains, is one of the most surface‐active biosurfactants, and it has potential applications in medicine, agriculture, and microbial enhanced oil recovery (MEOR) (Ashish & Debnath, ; Paraszkiewicz et al, ; Rodrigues, Banat, Teixeira, & Oliveira, ). However, due to its high production cost, its industrial applications are still underdeveloped.…”

Section: Introductionmentioning

confidence: 99%

Genome and transcriptome analysis of Bacillus velezensis BS‐37, an efficient surfactin producer from glycerol, in response to d‐/l‐leucine

Zhou

Lin

et al. 2019

MicrobiologyOpen

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Surfactin is one of the most widely studied biosurfactants due to its many potential applications in different fields. In the present study, Bacillus velezensis BS‐37, initially identified as a strain of Bacillus subtilis, was used to efficiently produce surfactin with the addition of glycerol, an inexpensive by‐product of biodiesel production. After 36 hr of growth in glycerol medium, the total surfactin concentration reached more than 1,000 mg/L, which was two times higher than that in sucrose medium. Moreover, the addition of l‐ and d‐Leu to the culture medium had opposite effects on surfactin production by BS‐37. While surfactin production increased significantly to nearly 2,000 mg/L with the addition of 10 mM l‐Leu, it was dramatically reduced to about 250 mg/L with the addition of 10 mM d‐Leu. To systemically elucidate the mechanisms influencing the efficiency of this biosynthesis process, we sequenced the genome of BS‐37 and analyzed changes of the transcriptome in glycerol medium in response to d‐/l‐leucine. The RPKM analysis of the transcriptome of BS‐37 showed that the transcription levels of genes encoding modular surfactin synthase, the glycerol utilization pathway, and branched‐chain amino acid (BCAA) synthesis pathways were all at a relatively high level, which may offered an explanation why this strain can efficiently use glycerol to produce surfactin with a high yield. Neither l‐Leu nor d‐Leu had a significant effect on the expression of genes in these pathways, indicating that l‐Leu plays an important role as a precursor or substrate involved in surfactin production, while d‐Leu appears to act as a competitive inhibitor. The results of the present study provide new insights into the synthesis of surfactin and ways of its regulation, and enrich the genomic and transcriptomic resources available for the construction of high‐producing strains.

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Agricultural potential of rhizospheric Bacillus subtilis strains exhibiting varied efficiency of surfactin production

Cited by 30 publications

References 37 publications

Halotolerant bacteria belonging to operational group Bacillus amyloliquefaciens in biocontrol of the rice brown stripe pathogen Acidovorax oryzae

Halotolerant bacteria belonging to operational group Bacillus amyloliquefaciens in biocontrol of the rice brown stripe pathogen Acidovorax oryzae

Characterization and complete genome analysis of the surfactin-producing, plant-protecting bacterium Bacillus velezensis 9D-6

Genome and transcriptome analysis of Bacillus velezensis BS‐37, an efficient surfactin producer from glycerol, in response to d‐/l‐leucine

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