Agrobacterium and biolistic transformation of onion using non-antibiotic selection marker phosphomannose isomerase

Aswath, C.; Mo, Sug Youn; Kim, Doo Hwan; Park, Se Won

doi:10.1007/s00299-005-0022-4

Cited by 47 publications

(28 citation statements)

References 23 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…This is a very high efficiency of transformation, when compared with reported data for other plant species. For example, TE reached 25 % in wheat , 30 % for maize (Negrotto et al 2000), 31 % for hemp (Feeney and Punja 2003), 41 % for rice (Lucca et al 2001), 23 % for cucumber (He et al 2006), 23 or 27 % for onion (Aswath et al 2006), 6.8 % for almond (Ramesh et al 2006) but only 0.72 % for pearl millet (O'Kennedy et al 2004), 0.94 % for sugar beet (Joersbo et al 1998), 1.14 % for durum wheat (Gadaleta et al 2006) or 3 -24 % for sweet orange (Boscariol et al 2003). When compared with kanamycin selection (TE = 33.3 %, Table 4) the transformation efficiency of optimal mannose selection procedure was about 1.5-fold higher.…”

Section: Tomato Transformationmentioning

confidence: 97%

“…The phosphomannose isomerase (pmi) gene was originally used as a selectable marker by Joersbo et al (1998) for the transformation of sugar beet. In the following years, PMI was shown to be an useful marker in the Agrobacterium transformation of a number of plant species such as cassava (Zhang et al 2000), maize (Negrotto et al 2000, Arabidopsis (Todd and Tague 2001), wheat , Gadaleta et al 2006, rice (Lucca et al 2001), sweet orange (Boscariol et al 2003), hemp (Feeney and Punja 2003), pearl millet (O'Kennedy et al 2004), bentgrass (Fu et al 2005), papaya (Zhu et al 2005), sorghum (Gao et al 2005), almond (Ramesh et al 2006), onion (Aswath et al 2006), cucumber (He et al 2006) and Chinese cabbage (Ku et al 2006). In tomato, Sigareva et al (2004) employed the mannose selection procedure for the transformation of cotyledon petiole, hypocotyls and leaf explants of three cultivars.…”

Section: Introductionmentioning

confidence: 97%

See 1 more Smart Citation

Use of phosphomannose isomerase-based selection system for Agrobacterium-mediated transformation of tomato and potato

Břı́za¹,

Pavingerová²,

Přikrylová³

et al. 2008

Biologia plant.

View full text Add to dashboard Cite

Two selection systems for Agrobacterium tumefaciens mediated transformation of tomato and potato were compared. In the tomato (Lycopersicon esculentum cv. Moneymaker), the highest transformation rate, 4.2 %, of cotyledon explants on mannose-selection medium was obtained when mannose/sucrose concentration in the regeneration medium was 5/15 g dm -3 . The best transformation efficacy with the commonly used concentration of 100 mg dm -3 kanamycin as a selection agent was 9 %. In the potato (Solanum tuberosum cv. Bintje), the highest transformation frequency was 53.3 % when mannose concentration in the regeneration medium was 5 g dm -3 during the first 3 weeks after transformation and 10 g dm -3 afterwards. The optimum concentration of sucrose was 20 g dm -3 . The transformation efficiency using kanamycin as a selection agent at a concentration 100 mg dm -3 was 33.3 % with potato. Our results demonstrate that the transformation efficiency using mannose selection is 1.6-fold higher for potato and about 2 times lower for tomato comparing with the ordinary protocol using kanamycin.

show abstract

Section: Tomato Transformationmentioning

confidence: 97%

Section: Introductionmentioning

confidence: 97%

Use of phosphomannose isomerase-based selection system for Agrobacterium-mediated transformation of tomato and potato

Břı́za¹,

Pavingerová²,

Přikrylová³

et al. 2008

Biologia plant.

View full text Add to dashboard Cite

show abstract

“…The mannose positive selection system favors the regeneration and growth of transgenic cells expressing the 6-phosphomannose isomerase (PMI) gene, manA, while the growth of non-transgenic cells is inhibited through carbohydrate starvation (positive selection). The utility of the pmi gene from Escherichia coli (Miles and Guest 1984) as a positive selectable marker in conjunction with mannose for recovery of transgenic plants (pmi/Man) has been demonstrated in a number of crops, including sugar beet (Joersbo et al 1998), cassava , maize (Wang et al 2000;Wright et al 2001), wheat , pepper (Kim et al 2002), sweet orange (Boscariol et al 2003), pearl millet (O'Kennedy et al 2004), tomato (Sigareva et al 2004), papaya (Zhu et al 2005), onion (Aswath et al 2006), almond (Ramesh et al 2006), cucumber (He et al 2006), maize (Privalle 2002), rice (He et al 2004), barley , apple (Degenhardt et al 2006), cabbage (Min et al 2007), sugarcane (Jain et al 2007), flax (Lamblin et al 2007), citrus (Ballester et al 2008), sorghum (Gurel et al 2009) and chickpea (Patil et al 2009). …”

Section: Introductionmentioning

confidence: 98%

Successful recovery of transgenic cowpea (Vigna unguiculata) using the 6-phosphomannose isomerase gene as the selectable marker

et al. 2012

View full text Add to dashboard Cite

A new method for obtaining transgenic cowpea was developed using positive selection based on the Escherichia coli 6-phosphomannose isomerase gene as the selectable marker and mannose as the selective agent. Only transformed cells were capable of utilizing mannose as a carbon source. Cotyledonary node explants from 4-day-old in vitro-germinated seedlings of cultivar Pusa Komal were inoculated with Agrobacterium tumefaciens strain EHA105 carrying the vector pNOV2819. Regenerating transformed shoots were selected on medium supplemented with a combination of 20 g/l mannose and 5 g/l sucrose as carbon source. The transformed shoots were rooted on medium devoid of mannose. Transformation efficiency based on PCR analysis of individual putative transformed shoots was 3.6%. Southern blot analysis on five randomly chosen PCR-positive plants confirmed the integration of the pmi transgene. Qualitative reverse transcription (qRT-PCR) analysis demonstrated the expression of pmi in T₀ transgenic plants. Chlorophenol red (CPR) assays confirmed the activity of PMI in transgenic plants, and the gene was transmitted to progeny in a Mendelian fashion. The transformation method presented here for cowpea using mannose selection is efficient and reproducible, and could be used to introduce a desirable gene(s) into cowpea for biotic and abiotic stress tolerance.

show abstract

“…The pmi/Man system has been successfully used in several plant systems such as sugar beet (Joersbo et al 1998), cassava (Zhang and Puonti-Kaerlas 2000), maize (Negrotto et al 2000;Wright et al 2001), Arabidopsis (Todd and Tague 2001), wheat , tobacco and potato (Kunze et al 2001), pepper (Kim et al 2002), sweet orange (Boscariol et al 2003), pearl millet (O'Kennedy et al 2004), tomato (Sigareva et al 2004, papaya (Zhu et al 2005), bentgrass (Fu et al 2005), apple (Degenhardt et al 2006), onion (Aswath et al 2006), almond (Ramesh et al 2006), cucumber (He et al 2006), cabbage (Min et al 2007), sugarcane (Jain et al 2007), flax (Lamblin et al 2007), citrus (Ballester et al 2008), sorghum (Gurel et al 2009) and chickpea (Patil et al 2009). However, the pmi/Man system has not been tested previously in Oncidium orchid.…”

Section: Introductionmentioning

confidence: 99%

Phosphomannose-isomerase as a selectable marker to recover transgenic orchid plants (Oncidium Gower Ramsey)

Thiruvengadam

Hsu

Yang

2010

Plant Cell Tiss Organ Cult

View full text Add to dashboard Cite

A transformation method using the phosphomannose-isomerase (pmi) gene as a selectable marker was developed for orchid Oncidium Gower Ramsey. The pmigene, which converts mannose-6-phosphate to fructose-6-phosphate allowing for selection of transgenic plants on mannose selective medium. Genetically transformed plants of Oncidium were regenerated after cocultivating protocorm-like bodies with Agrobacterium tumefaciens strain GV3101 containing the vectors pEPYON-42P and pEP-YON-42H with 35S::PMI and 35S::HPTII genes respectively. We observed that 35S::PMI (pEPYON-42P) produced high rate (27 plants) of mannose resistant transgenic plants compared to 35S::HPTII (pEPYON-42H) in which only fourteen hygromycin resistant transgenic plants were obtained. Mannose resistant transgenic plants were confirmed by PCR and Southern blot. The pmi gene expression in 35S::PMI (pEPYON-42P) transgenic plants was confirmed by RT-PCR. Furthermore, the duration of regeneration time of transgenic plants was significantly shorter in mannose selected system (4 months) than in hygromycin selected system (8 months). The pmi/mannose selection system is shown to be highly efficient for producing transgenic O. Gower Ramsey without using antibiotics or herbicides. For the first time, the pmi/ mannose-based ''positive'' selection system has been used to obtain genetically engineered O. Gower Ramsey.

show abstract

Agrobacterium and biolistic transformation of onion using non-antibiotic selection marker phosphomannose isomerase

Cited by 47 publications

References 23 publications

Use of phosphomannose isomerase-based selection system for Agrobacterium-mediated transformation of tomato and potato

Use of phosphomannose isomerase-based selection system for Agrobacterium-mediated transformation of tomato and potato

Successful recovery of transgenic cowpea (Vigna unguiculata) using the 6-phosphomannose isomerase gene as the selectable marker

Phosphomannose-isomerase as a selectable marker to recover transgenic orchid plants (Oncidium Gower Ramsey)

Contact Info

Product

Resources

About