Agrobacterium Bioassay Strain for Ultrasensitive Detection of
 N
 -Acylhomoserine Lactone-Type Quorum-Sensing Molecules: Detection of Autoinducers in
 Mesorhizobium huakuii

Zhu, Jun; Chai, Yunrong; Zhong, Zengtao; Li, Shunpeng; Winans, Stephen C.

doi:10.1128/aem.69.11.6949-6953.2003

Cited by 201 publications

(219 citation statements)

References 46 publications

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“…A136 is a Gram-negative soil bacterium which carries a lacZ fusion to the traI and cannot produce its own AHLs, but can induce traI:lacZ reporter in the presence of exogenous AHLs. A136 detects a broad range of AHLs with high sensitivity to these compounds (Zhu et al, 2003). CV026 is a double mini-Tn5 mutant bacterial strain which regulates the violacein production via the CviI/R AHL QS system in response to exogenous AHLs (McClean et al, 1997).…”

Section: Discussionmentioning

confidence: 99%

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Characterization of N-acyl homoserine lactones (AHLs) producing bacteria isolated from vacuum-packaged refrigerated turbot (Scophthalmus maximus) and possible influence of exogenous AHLs on bacterial phenotype

Zhang

Zhu

Jatt

et al. 2016

J. Gen. Appl. Microbiol.

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Quorum sensing (QS) is a cell-to-cell communication mechanism through which microbial cells communicate and regulate their wide variety of biological activities. N-acyl homoserine lactones (AHLs) are considered to be the most important QS signaling molecules produced by several Gramnegative bacteria. The present study aimed to screen the AHLs-producing bacteria from spoiled vacuum-packaged refrigerated turbot (Scophthalmus maximus) by biosensor assays, and the profiles of AHLs produced by these bacteria were determined using reversed-phase thin-layer chromatography (RP-TLC) and gas chromatography-mass spectrometry (GC-MS). Effects of exogenous AHLs and QS inhibitor (QSI) on the phenotypes (i.e., extracellular proteolytic activity and biofilm formation) of the AHLs-producing bacteria were also evaluated. Our results demonstrated that eight out of twenty-two isolates were found to produce AHLs. Three of the AHLs-producing isolates were identified as Serratia sp., and the other five were found to belong to the family of Aeromonas. Two isolates (i.e., S. liquefaciens A2 and A. sobria B1) with higher AHLs-producing activities were selected for further studies. Mainly, RP-TLC and GC-MS analysis revealed three AHLs, i.e., 3-oxo-C6-HSL, C8-HSL and C10-HSL were produced by S. liquefaciens A2, while five AHLs, i.e., C4-HSL, C6-HSL, C8-HSL, C10-HSL, and C12-HSL, were produced by A. sobria B1. Moreover, production of AHLs in both bacterial strains were found to be density-dependent, and the AHLs activity reached a maximum level in their middle logarithmic phase and decreased in the stationary phase. The addition of exogenous AHLs and QSI decreased the specific protease activity both of the Serratia A2 and Aeromonas B1. Exogenous AHLs inhibited the biofilm formation of Serratia A2 while it enhanced the biofilm formation in Aeromonas B1. QSI inhibited the specific protease activity and biofilm formation in both bacterial strains.

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Section: Discussionmentioning

confidence: 99%

“…They were grown in Luria-Bertani (LB) broth at 28°C. The growth medium for A136 was further supplemented with appropriate antibiotics, i.e., spectinomycin (50 µg mL -1 ) and tetracycline (4.5 µg mL -1 ) (McClean et al, 1997;Zhu et al, 2003). Isolation of bacterial strains.…”

Section: Methodsmentioning

confidence: 99%

Characterization of N-acyl homoserine lactones (AHLs) producing bacteria isolated from vacuum-packaged refrigerated turbot (Scophthalmus maximus) and possible influence of exogenous AHLs on bacterial phenotype

Zhang

Zhu

Jatt

et al. 2016

J. Gen. Appl. Microbiol.

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“…Culture supernatants of test strains were filter sterilized (0.22 mm) and 60 ml of sterile supernatant was added to 140 ml AB medium (Greenberg et al, 1979) containing V. harveyi TL-26 (inoculated 1:5000 from an overnight culture). Agrobacterium tumefaciens KYC55, which does not produce AI-2 (Zhu et al, 2003), was used as a control to test if this assay is specific. Vibrio mimicus ATCC 33653 was used as a positive control.…”

Section: Amplification Of Partial Luxs Gene Sequences From Vibrio Isomentioning

confidence: 99%

Diversity and functional analysis of luxS genes in Vibrios from marine sponges Mycale laxissima and Ircinia strobilina

2011

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Sponges harbor highly diverse and dense microbial communities, providing an environment in which bacterial signaling may be important. Quorum sensing (QS) is a cell density-dependent signaling process that bacteria employ to coordinate and regulate their gene expression. Previous studies have found that bacteria isolated from sponges are able to produce acyl-homoserine lactones (AHLs), an important class of QS molecules found in proteobacteria. Autoinducer-2 (AI-2) is a second class of QS molecule, and is considered to be an interspecies signal. However, AI-2 signaling has not been reported in sponge bacterial symbionts. In this study, degenerate primers were designed based on known Vibrio luxS sequences to amplify the luxS genes encoding AI-2 synthases of several Vibrio isolates from marine sponges Mycale laxissima and Ircinia strobilina. All the vibrios isolated from these two sponges had luxS genes and were able to produce signals with AI-2 activity as detected using a biological reporter. A novel group of luxS sequences was found, thus extending the known diversity of luxS genes. One isolate was chosen for further analysis of its luxS gene by expression of the gene in Escherichia coli DH5a and by characterization of the profile of AI-2 activity. This work provides the first information about luxS genes and AI-2 activity in sponge-associated bacterial communities.

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“…The level of AHLs in cell-free culture fluids of Ei-151 at various time points was quantified by examining the ability of samples to activate TraR in KYC55, as described by Zhu et al [18]. Fifty microliters of oxo-C 8 -HSL (OOHL) (50 lg ml -1 ) was used as a positive control, while the fresh BHI media was the negative control.…”

Section: Growth Phase-dependent Analysis Of Ahls Production In Liquidmentioning

confidence: 99%

Detection of Quorum Sensing Signal Molecules in Edwardsiella ictaluri Ei-151

et al. 2012

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Edwardsiella ictaluri is a Gram-negative pathogenic bacterium in the family Enterobacteriaceae that causes enteric septicemia of catfish, which has become a significant problem in the aquaculture of striped catfish (Pangasianodon hypophthalmus) in Vietnam. In this study, a bacterium designated as Ei-151 was isolated from diseased striped catfish and proved to be virulent. Based on 16S rDNA sequencing and phenotypic tests, the pathogenic bacterium was identified as Edw. ictaluri. The presence of quorum sensing signal molecules in Edw. ictaluri Ei-151 was detected with different biosensor strains. The results showed that Ei-151 produced at least three kinds of acylated homoserine lactone (AHL) signal molecules as detected with the biosensor Agrobacterium tumefaciens KYC55, and the AHLs fingerprint was similar to that of Edw. tarda. During its entire growth, the levels of AHLs and autoinducer-2 produced by Ei-151 peaked at the stationary phase (OD 600

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Agrobacterium Bioassay Strain for Ultrasensitive Detection of N -Acylhomoserine Lactone-Type Quorum-Sensing Molecules: Detection of Autoinducers in Mesorhizobium huakuii

Cited by 201 publications

References 46 publications

Characterization of <i>N</i>-acyl homoserine lactones (AHLs) producing bacteria isolated from vacuum-packaged refrigerated turbot (<i>Scophthalmus maximus</i>) and possible influence of exogenous AHLs on bacterial phenotype

Characterization of <i>N</i>-acyl homoserine lactones (AHLs) producing bacteria isolated from vacuum-packaged refrigerated turbot (<i>Scophthalmus maximus</i>) and possible influence of exogenous AHLs on bacterial phenotype

Diversity and functional analysis of luxS genes in Vibrios from marine sponges Mycale laxissima and Ircinia strobilina

Detection of Quorum Sensing Signal Molecules in Edwardsiella ictaluri Ei-151

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