Agrobacterium-mediated transformation of Cry1C, Cry2A and Cry9C genes into Gossypium hirsutum and plant regeneration

Guo, Xiaoping; Huang, Chao; Jin, Shuangxia; Liang, Shuang; Nie, Yong; Zhang, X.

doi:10.1007/s10535-007-0048-2

Cited by 16 publications

(5 citation statements)

References 22 publications

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“…To generate plants that express the desired traits, different techniques for inserting genes into plants have been developed since the early 1980s ( De Block et al, 1984 ) and are currently widely used and commercially available ( James, 2014 ). Cotton transformation methods normally use co-cultures with A. tumefaciens ( Guo et al, 2007 ) and microprojectile bombardment ( Aragão et al, 2005 ). It is well known that the transformation efficacy is affected by the plant material, genotype/variety, and type of explant ( Wilkins et al, 2004 ; Rao et al, 2009 ; Anami et al, 2013 ; Chakravarthy et al, 2014 ; Juturu et al, 2015 ).…”

Section: Discussionmentioning

confidence: 99%

Transgenic Cotton Plants Expressing Cry1Ia12 Toxin Confer Resistance to Fall Armyworm (Spodoptera frugiperda) and Cotton Boll Weevil (Anthonomus grandis)

et al. 2016

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Gossypium hirsutum (commercial cooton) is one of the most economically important fibers sources and a commodity crop highly affected by insect pests and pathogens. Several transgenic approaches have been developed to improve cotton resistance to insect pests, through the transgenic expression of different factors, including Cry toxins, proteinase inhibitors, and toxic peptides, among others. In the present study, we developed transgenic cotton plants by fertilized floral buds injection (through the pollen-tube pathway technique) using an DNA expression cassette harboring the cry1Ia12 gene, driven by CaMV35S promoter. The T0 transgenic cotton plants were initially selected with kanamycin and posteriorly characterized by PCR and Southern blot experiments to confirm the genetic transformation. Western blot and ELISA assays indicated the transgenic cotton plants with higher Cry1Ia12 protein expression levels to be further tested in the control of two major G. hirsutum insect pests. Bioassays with T1 plants revealed the Cry1Ia12 protein toxicity on Spodoptera frugiperda larvae, as evidenced by mortality up to 40% and a significant delay in the development of the target insects compared to untransformed controls (up to 30-fold). Also, an important reduction of Anthonomus grandis emerging adults (up to 60%) was observed when the insect larvae were fed on T1 floral buds. All the larvae and adult insect survivors on the transgenic lines were weaker and significantly smaller compared to the non-transformed plants. Therefore, this study provides GM cotton plant with simultaneous resistance against the Lepidopteran (S. frugiperda), and the Coleopteran (A. grandis) insect orders, and all data suggested that the Cry1Ia12 toxin could effectively enhance the cotton transgenic plants resistance to both insect pests.

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Section: Discussionmentioning

confidence: 99%

Transgenic Cotton Plants Expressing Cry1Ia12 Toxin Confer Resistance to Fall Armyworm (Spodoptera frugiperda) and Cotton Boll Weevil (Anthonomus grandis)

et al. 2016

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“…In cotton plant, transgenic technology has been mainly used to induce the resistance against insect herbivores. Among the different transgenic approaches against insects, Cry gene encoding Bacillus thuringiensis toxin has gained a fabulous success against bollworms (Guo et al, 2007a;Rashid et al, 2008). Recently, tma-12 gene encoding insecticidal protein has been identified that gives substantial results against whitefly and cotton leaf curl viral disease (Shukla et al, 2016).…”

Section: Biotic Stressesmentioning

confidence: 99%

Recent insights into cotton functional genomics: progress and future perspectives

Ashraf

Zuo

Wang

et al. 2018

Plant Biotechnology Journal

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SummaryFunctional genomics has transformed from futuristic concept to well‐established scientific discipline during the last decade. Cotton functional genomics promise to enhance the understanding of fundamental plant biology to systematically exploit genetic resources for the improvement of cotton fibre quality and yield, as well as utilization of genetic information for germplasm improvement. However, determining the cotton gene functions is a much more challenging task, which has not progressed at a rapid pace. This article presents a comprehensive overview of the recent tools and resources available with the major advances in cotton functional genomics to develop elite cotton genotypes. This effort ultimately helps to filter a subset of genes that can be used to assemble a final list of candidate genes that could be employed in future novel cotton breeding programme. We argue that next stage of cotton functional genomics requires the draft genomes refinement, re‐sequencing broad diversity panels with the development of high‐throughput functional genomics tools and integrating multidisciplinary approaches in upcoming cotton improvement programmes.

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“…Hussain et al (2007) transformed a Pakistani cotton cultivar with snow drop Galanthus nivalis (GNA) and cry1Ac gene to encode resistance against sucking as well as chewing insect pests. Transgenic cotton lines expressing cry1C, cry2A and cry9C showed enhanced resistance against Heliothis armigera (Guo et al 2007). The efficacy of cry1F insecticidal protein was deemed satisfactory for the control of fall armyworm (Spodoptera frugiperda) in cotton by Siebert et al (2008) under field conditions.…”

Section: Insect-resistant Cottonmentioning

confidence: 99%

An insight into cotton genetic engineering (Gossypium hirsutum L.): current endeavors and prospects

Bakhsh

Anayol²,

Özcan³

et al. 2015

Acta Physiol Plant

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Cotton (Gossypium hirsutum L.) is the most significant cash crop and backbone of global textile industry. The importance of cotton can hardly be over emphasized in the economy of cotton-growing countries as cotton and cotton products contribute significantly to the foreign exchange earnings. Cotton breeders have continuously sought to improve cotton's quality through conventional breeding in the past centuries; however, due to limited availability of germplasm with resistant to particular insects, pests and diseases, further advancements in cotton breeding have been challenging. The progress in transformation systems in cotton paved the way for the genetic improvement by enabling the researchers to transfer specific genes among the species and to incorporate them in cotton genome. With the development of first genetically engineered cotton plant in 1987, several characteristics such as biotic (insects, viruses, bacteria and fungi) resistance, abiotic (drought, chilling, heat, salt), herbicide tolerance, manipulation of oil and fiber traits have been reported to date. Genetic engineering has emerged as a necessary tool in cotton breeding programs, strengthening classical strategies to improve yield and yield contributing factors. The current review highlights the advances and endeavors in cotton genetic engineering achieved by researchers worldwide utilizing modern biotechnological approaches. Future prospects of the transgenic cotton are also discussed.

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Agrobacterium-mediated transformation of Cry1C, Cry2A and Cry9C genes into Gossypium hirsutum and plant regeneration

Cited by 16 publications

References 22 publications

Transgenic Cotton Plants Expressing Cry1Ia12 Toxin Confer Resistance to Fall Armyworm (Spodoptera frugiperda) and Cotton Boll Weevil (Anthonomus grandis)

Transgenic Cotton Plants Expressing Cry1Ia12 Toxin Confer Resistance to Fall Armyworm (Spodoptera frugiperda) and Cotton Boll Weevil (Anthonomus grandis)

Recent insights into cotton functional genomics: progress and future perspectives

An insight into cotton genetic engineering (Gossypium hirsutum L.): current endeavors and prospects

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