Agrobacterium-mediated transformation of élite indica and japonica rice cultivars

Zhang, Jian; Xu, Rui‐ji; Elliott, Malcolm C.; Chen, Dongfang

doi:10.1007/bf02760776

Cited by 42 publications

(16 citation statements)

References 17 publications

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“…This was likely due to the presence of the additional virB, C and G genes from pTiBo542 (Hood et al, 1986;Jin et al, 1987;Komari, 1990) on pTOK233 (Hiei et al, 1994). Similar results have been reported following inoculation of mature embryo-derived calluses (Hiei et al, 1994;Zhang et al, 1997;Azhakanandam et al, 2000), or immature embryos (Aldemita and Hodges, 1996;Dong et al, 1996) in rice transformation.…”

Section: Discussionsupporting

confidence: 86%

Agrobacterium-mediated transformation of Indica rice genotypes: an assessment of factors affecting the transformation efficiency

Hoque

Mansfıeld

Bennett

2005

Plant Cell Tiss Organ Cult

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An efficient Agrobacterium-mediated method for transformation of popular Bangladeshi Indica rice genotypes has been developed. Mature embryo-derived calluses as well as immature embryos were used as the target material. Transgenic plant production frequency was higher using the immature embryos than mature embryo-derived calluses. However, 3-week-old mature embryo-derived calluses served as an excellent starting material. The super-binary vector (pTOK233) was generally more effective than the binary vector (pC1301-Xa21mSS) particularly with recalcitrant Bangladeshi genotypes such as BR22. However, transformation of the Japonica cultivar Taipei-309 was equally effective with either plasmid. Inclusion of acetosyringone (200 lM) in co-cultivation media proved essential for successful transformation and the optimum co-cultivation period found was to be 3 days. A large number of morphologically normal, fertile transgenic plants were obtained which expressed gus as determined by histochemical staining. Integration of the hpt gene into the genome of transgenic plants was confirmed by molecular analysis. Mendelian inheritance of transgenes (hpt and gus gene) was observed in T 1 progeny.

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Section: Discussionsupporting

confidence: 86%

Agrobacterium-mediated transformation of Indica rice genotypes: an assessment of factors affecting the transformation efficiency

Hoque

Mansfıeld

Bennett

2005

Plant Cell Tiss Organ Cult

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“…Agrobacterium-mediated transformation and microparticle bombardment are the two most widely used methods for genetic transformation.The former has been an established protocol for many years, first in dicot crops ) and more recently in major cereal crops such as rice (Chan et al1992(Chan et al , 1993Hiei et al 1994;Aldemita and Hodges 1996;Dong et al1996;Zhang et al 1997), corn (Ishida et al 1996;Komari et al 1996), barley (Tingay et al 1997) and wheat (Cheng et al 1997). Agrobacterium transformation is the method of choice because it allows for the stable integration of a defined segment of DNA into the plant genome and generally results in a lower copy number, fewer rearrangements and an improved stability of expression over generations than the free DNA delivery methods (Smith and Hood 1995;Dai et al 2001).…”

Section: Introductionmentioning

confidence: 99%

Agrobacterium-mediated large-scale transformation of wheat (Triticum aestivum L.) using glyphosate selection

et al. 2003

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An Agrobacterium-mediated transformation system with glyphosate selection has been developed for the large-scale production of transgenic plants. The system uses 4-day precultured immature embryos as explants. A total of 30 vectors containing the 5-enol-pyruvylshikimate-3-phosphate synthase gene from Agrobacterium strain CP4 (aroA:CP4), which confers resistance to glyphosate, were introduced into wheat using this system. The aroA:CP4 gene served two roles in this study-selectable marker and gene of interest. More than 3,000 transgenic events were produced with an average transformation efficiency of 4.4%. The entire process from isolation of immature embryos to production of transgenic plantlets was 50-80 days. Transgenic events were evaluated over several generations based on genetic, agronomic and molecular criteria. Forty-six percent of the transgenic events fit a 3:1 segregation ratio. Molecular analysis confirmed that four of six lead transgenic events selected from Agrobacterium transformation contained a single insert and a single copy of the transgene. Stable expression of theAROA:CP4 gene was confirmed by ELISA through nine generations. A comparison of Agrobacterium-mediated transformation to a particle bombardment system demonstrated that the Agrobacterium system is reproducible, has a higher transformation efficiency with glyphosate selection and produces higher quality transgenic events in wheat. One of the lead events from this study, no. 33391, has been identified as a Roundup Ready wheat commercial candidate.

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“…Aldemita and Hodges (1996) succeeded in the Agrobacterium-mediated transformation of Group I varieties for the first time and the transformation frequency (independent transgenic plants per immature embryo infected) was 0.7% for TSC10 and 4.2% for IR72. Successful transformation of callus cultures mediated by Agrobacterium was also reported in Pusa Basmati I (Zhang et al, 1997;Mohanty et al, 1999;Azhakanandam et al, 2000;Kumria et al, 2001;Kumria and Rajam, 2002) and IR64 (Khanna and Raina, 2002). The transformation frequency (independent transgenic plants per callus piece infected) of IR64 was between 2.5 and 10.1% (Khanna and Raina, 2002).…”

Section: Introductionmentioning

confidence: 57%

Improved protocols for transformation of indica rice mediated by Agrobacterium tumefaciens

Hiei

Komari

2006

Plant Cell Tiss Organ Cult

103

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A highly efficient gene transfer method mediated by Agrobacterium tumefaciens was developed for Group I indica rice, which had been quite recalcitrant in tissue culture and transformation. Freshly isolated immature embryos from plants grown in a greenhouse were inoculated with A. tumefaciens LBA4404 that harbored super-binary vector pTOK233 or pSB134, which had a hygromycin-resistance gene and a GUS gene in the T-DNA. The efficiency of gene transfer varied with the kinds of gelling agents and the basic compositions of co-cultivation media. The highest activity of GUS after co-cultivation was observed when NB medium solidified with agarose was used. For the subsequent cultures, two types of media (modified NB and CC) were chosen to recover hygromycin-resistant cells efficiently. The transformation protocol thus developed worked very well in all of the varieties tested in this study, and the transformation frequency (number of independent hygromycin-resistant and GUS-positive plants per embryo) reached more than 30% in IR8, IR24, IR26, IR36, IR54, IR64, IR72, Xin Qing Ai 1, Nan Jin 11, and Suewon 258. Most of the transformants (T 0 ) were normal in morphology and fertile. Stable integration, expression and inheritance of transgenes were demonstrated by molecular and genetic analysis of transformants in the T 0 and T 1 generations. For the recovery of multiple independent transgenic events from a single immature embryo, procedures were developed to section the embryo into as many as 30 pieces after non-selective cultures following co-cultivation. Transformants were then obtained from the pieces cultured on the selective media, and, in the highest case, more than seven independent transgenic plants per original embryo (transformation frequency of 738%) were produced. Thus, the efficiency of transformation was remarkably improved.

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Agrobacterium-mediated transformation of élite indica and japonica rice cultivars

Cited by 42 publications

References 17 publications

Agrobacterium-mediated transformation of Indica rice genotypes: an assessment of factors affecting the transformation efficiency

Agrobacterium-mediated transformation of Indica rice genotypes: an assessment of factors affecting the transformation efficiency

Agrobacterium-mediated large-scale transformation of wheat (Triticum aestivum L.) using glyphosate selection

Improved protocols for transformation of indica rice mediated by Agrobacterium tumefaciens

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