Agrobacterium-mediated transformation of Indica rice genotypes: an assessment of factors affecting the transformation efficiency

Hoque, Md Enamul; Mansfıeld, John W.; Bennett, Mark H.

doi:10.1007/s11240-004-6154-3

Cited by 28 publications

(22 citation statements)

References 37 publications

(45 reference statements)

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“…* -significant at P < 0.5 of 48 h resulted in efficient transformation, which has also been reported in canola (Cardoza and Stewart, 2003). In contrarst, the optimum cocultivation period of 72 h (Hoque et al, 2005) proved essential for successful transformation.…”

Section: Discussionmentioning

confidence: 99%

Ploidy stability of Oryza sativa. L cv IR64 transformed with the moth bean P5CS gene with significant tolerance against drought and salinity

Priya¹,

Krishnan²,

Ramesh³

2015

Turk J Biol

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Rice is one of the world's most important crops. Thus, it is necessary to improve it by genetic transformation. IR64 is a highyielding and disease-resistant variety, but it is sensitive to abiotic stresses, mainly salinity and drought. Therefore, an efficient transformation system is a prerequisite for transforming this indica rice with a stress-tolerant gene, the potent osmolyte proline-coding P5CS gene of Vigna aconitifolia. Successful transgene integration was confirmed by polymerase chain reaction (PCR) and Southern blot analysis. A Mendelian pattern of transgene inheritance was also observed in the progeny plants. The individual transformants expressed significant tolerance over wild-type control plants, as exhibited by NaCl tolerance, lipid peroxidation levels, and total chlorophyll content. Percentage of germination, height, and weight of the plantlet at 400 mM mannitol stress was 60%, 8.1 cm, and 92.5 mg, respectively. Total chlorophyll retention and malondialdehyde content at 200 mM NaCl stress was 65.3% and 8.2 n mol g -1 FW, respectively. These results revealed the abiotic stress tolerance conferred by Δ1-pyrroline-5-carboxylate synthetase. Additionally, flow cytometry analysis confirmed the absence of somaclonal variation. Hence, an efficient genetic transformation can be accomplished in other indica rice cultivars by using this protocol.

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Section: Discussionmentioning

confidence: 99%

Ploidy stability of Oryza sativa. L cv IR64 transformed with the moth bean P5CS gene with significant tolerance against drought and salinity

Priya¹,

Krishnan²,

Ramesh³

2015

Turk J Biol

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“…This bacteria restricts annual production of rice in both tropical and temperate regions of the world [5]. In the tropics, the damage is more severe than in the temperate regions [6]. The BLB disease incidence has been recorded in various parts of Asia, USA, Africa, and northern Australia [7].…”

Section: Introductionmentioning

confidence: 99%

Mycosynthesis of ZnO Nanoparticles Using Trichoderma spp. Isolated from Rhizosphere Soils and Its Synergistic Antibacterial Effect against Xanthomonas oryzae pv. oryzae

Shobha

Lakshmeesha

Ansari

et al. 2020

JoF

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The Plant Growth Promoting Fungi (PGPF) is used as a source of biofertilizers due to their production of secondary metabolites and beneficial effects on plants. The present work is focused on the co-cultivation of Trichoderma spp. (T. harzianum (PGT4), T. reesei (PGT5) and T. reesei (PGT13)) and the production of secondary metabolites from mono and co-culture and mycosynthesis of zinc oxide nanoparticles (ZnO NPs), which were characterized by a UV visible spectrophotometer, Powder X-ray Diffraction (PXRD), Fourier Transform Infrared Spectroscopy (FTIR) and Scanning Electron Microscopy (SEM) with Energy Dispersive Spectroscopy (EDAX) and Transmission Electron Microscope (TEM) and Selected Area (Electron) Diffraction (SAED) patterns. The fungal secondary metabolite crude was extracted from the mono and co-culture of Trichoderma spp. And were analyzed by GC-MS, which was further subjected for antibacterial activity against Xanthomonas oryzae pv. Oryzae, the causative organism for Bacterial Leaf Blight (BLB) in rice. Our results showed that the maximum zone of inhibition was recorded from the co-culture of Trichoderma spp. rather than mono cultures, which indicates that co-cultivation of beneficial fungi can stimulate the synthesis of novel secondary metabolites better than in monocultures. ZnO NPs were synthesized from fungal secondary metabolites of mono cultures of Trichoderma harzianum (PGT4), Trichoderma reesei (PGT5), Trichoderma reesei (PGT13) and co-culture (PGT4 + PGT5 + PGT13). These ZnO NPs were checked for antibacterial activity against Xoo, which was found to be of a dose-dependent manner. In summary, the biosynthesized ZnO NPs and secondary metabolites from co-culture of Trichoderma spp. are ecofriendly and can be used as an alternative for chemical fertilizers in agriculture.

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“…India is second only to China in rice production with more than 129 million tons in 2005 (FAOSTAT, 2006). In addition to its food values and economic importance rice, with its relatively small genome size together with its complete genome sequence (Sasaki et al, 2005), is considered as a model monocot system for various biotechnological, metabolic, genetic engineering and functional genomics development studies worldwide (Bajaj and Mohanty, 2005;Hoque et al, 2005;Ge et al, 2006).…”

Section: Introductionmentioning

confidence: 99%

Sodium Chloride-Induced Changes in Mineral Nutrients and Proline Accumulation in Indica Rice Cultivars Differing in Salt Tolerance

Kumar

Shriram

Nikam

et al. 2008

Journal of Plant Nutrition

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The effect of increasing sodium chloride (NaCl; 0 to 300 mM) stress was investigated on plant growth, mineral nutrients, and proline accumulation in two indica rice cultivars differing in salt tolerance. The shoots and roots of 'Karjat-3' (salt sensitive cv.) showed greater reduction in fresh weight, dry weight, and water content under increasing salinity stress when compared to 'Panvel-3' (salt tolerant cv.). The magnitude of chloride (Cl) accumulation with increasing salinity varied between cultivars, with lesser accumulation in 'Panvel-3', whereas, calcium (Ca) content was more in 'Panvel-3' than 'Karjat-3' at all salinity levels. Increase in shoot-sodium (Na) did not show notable variation between cultivars under salinity stress; however, Na accumulation was notably lower in roots of 'Panvel-3' than 'Karjat-3'. Under increasing salinity stress, 'Panvel-3' maintained significantly lower sodium/potassium, sodium/calcium and sodium/nitrogen ratios than 'Karjat-3', both in shoots and roots, with more differential response in roots. Regardless of treatment, proline concentration was considerably higher in 'Panvel-3' than in 'Karjat-3'. The salt tolerance of cv. 'Panvel-3' was positively correlated with better growth, higher proline accumulation and enhanced uptakes of beneficial minerals such as potassium (K) and Ca.

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Agrobacterium-mediated transformation of Indica rice genotypes: an assessment of factors affecting the transformation efficiency

Cited by 28 publications

References 37 publications

Ploidy stability of Oryza sativa. L cv IR64 transformed with the moth bean P5CS gene with significant tolerance against drought and salinity

Ploidy stability of Oryza sativa. L cv IR64 transformed with the moth bean P5CS gene with significant tolerance against drought and salinity

Mycosynthesis of ZnO Nanoparticles Using Trichoderma spp. Isolated from Rhizosphere Soils and Its Synergistic Antibacterial Effect against Xanthomonas oryzae pv. oryzae

Sodium Chloride-Induced Changes in Mineral Nutrients and Proline Accumulation in Indica Rice Cultivars Differing in Salt Tolerance

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