Agrobacterium-mediated transformation of Hyacinthus orientalis with thaumatin II gene to control fungal diseases

Popowich, E. A.; Фирсов, А. А.; Mitiouchkina, Tatiana; Filipenya, V. L.; Dolgov, Sergey; Reshetnikov, V. N.

doi:10.1007/s11240-007-9254-z

Cited by 21 publications

(9 citation statements)

References 18 publications

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“…In previous analysis it was described that 224 line possess increased tolerance to downy mildew [12,22]. This is in line with other analyses of transgenic plants with thaumatin II transgene and enhancement of pathogen resistance [17,57].…”

Section: Discussionsupporting

confidence: 77%

Effect of Transgenesis on mRNA and miRNA Profiles in Cucumber Fruits Expressing Thaumatin II

Pawełkowicz

Skarzyńska

Sroka

et al. 2020

Genes

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Transgenic plants are commonly used in breeding programs because of the various features that can be introduced. However, unintended effects caused by genetic transformation are still a topic of concern. This makes research on the nutritional safety of transgenic crop plants extremely interesting. Cucumber (Cucumis sativus L.) is a crop that is grown worldwide. The aim of this study was to identify and characterize differentially expressed genes and regulatory miRNAs in transgenic cucumber fruits that contain the thaumatin II gene, which encodes the sweet-tasting protein thaumatin II, by NGS sequencing. We compared the fruit transcriptomes and miRNomes of three transgenic cucumber lines with wild-type cucumber. In total, we found 47 differentially expressed genes between control and all three transgenic lines. We performed the bioinformatic functional analysis and gene ontology classification. We also identified 12 differentially regulated miRNAs, from which three can influence the two targets (assigned as DEGs) in one of the studied transgenic lines (line 224). We found that the transformation of cucumber with thaumatin II and expression of the transgene had minimal impact on gene expression and epigenetic regulation by miRNA, in the cucumber fruits.

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Section: Discussionsupporting

confidence: 77%

Effect of Transgenesis on mRNA and miRNA Profiles in Cucumber Fruits Expressing Thaumatin II

Pawełkowicz

Skarzyńska

Sroka

et al. 2020

Genes

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“…Shoot tips were used as explants in Agrobacteriummediated transformation of Gladiolus (Babu and Chawla, 2000). Leaf explants also resulted in successful transformation of Hyacinthus orientalis and Narcissus tazzeta (Lu et al, 2007;Popowich et al, 2007). The most common way of stable transformation of monocotyledonous geophytes has been through the use of callus (Table 2).…”

Section: Explant Age/sourcementioning

confidence: 99%

“…H. orientalis cv. Chine Pink transformed with the thaumatin II gene showed a significant level of resistance to the pathogenic fungus; Botrytis cinera (Popowich et al, 2007). Gladiolus plants transformed with a defective replicase and protein subgroup II gene were found to be resistant to cucumber mosaic virus (CMV) (Kamo et al, 2010), while Azadi et al (2011) established that the integration of a defective CMV replicase gene (CMV2-GDD) resulted in virus resistant Lilium plants.…”

Section: Past Efforts and Achievements In Transformation Of Monocotylmentioning

confidence: 99%

A review on factors affecting the Agrobacterium-mediated genetic transformation in ornamental monocotyledonous geophytes

Koetle

Finnie

Balázs

et al. 2015

South African Journal of Botany

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Genetic improvement of ornamental geophytes especially the monocotyledonous type; is often restricted by failure of Agrobacterium to reach competent cells as well as a lack of efficient regeneration systems. Despite all these limitations, it has recently been shown that the use of efficient promoters, super-virulent strains, and the utilization of systems such as an agrobacterial monolayer, Agrobacterium-mediated pollen and seed transformation, floral dip method and sonication-assisted Agrobacterium-mediated transformation (SAAT) will ensure success in the genetic transformation of these ornamentals in the near future. This article outlines factors affecting transformation of monocotyledonous geophytes. Special emphasis is laid on measures that have been employed to alleviate various difficulties. The need to develop somatic embryogenesis protocols for the ease of transformation is highlighted. In addition, perspectives in view of future research are also given. This information is crucial for biotechnological improvement of ornamental geophytes that are proving difficult to transform.

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“…PR‐5 proteins in fruit species are often induced upon fruit ripening, when the sugar increases, suggesting a possible secondary role in ripening in addition to its antifungal activity (Fils‐Lycaon and others 1996; Barre and others 2000). Several studies have shown that transgenic lines of different crops expressing the thaumatin II gene exhibit enhanced tolerance to environmental stresses (Szwacka and others 2002; Schestibratov and Dolgov 2005; Popovich and others 2007; Rajam and others 2007).…”

Section: Introductionmentioning

confidence: 99%

Genetically Modified Crops Expressing 35S‐Thaumatin II Transgene: Sensory Properties and Food Safety Aspects

Szwacka¹,

Burza²,

Zawirska‐Wojtasiak³

et al. 2012

Comp Rev Food Sci Food Safe

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Obesity and diabetes have led consumers to increasingly seek out products containing low-calorie sweeteners. In this context, the intense sweeteners, especially plant proteins that interact with the taste receptors, are playing a growing role. Among the sweet-tasting proteins thaumatin seems the most promising. Production of thaumatin by transgenic plants might be an interesting alternative to the extraction of this product from the natural source. Another possible application of the unusual properties of thaumatin is to produce transgenic plants containing a protein capable of inducing a sweet taste phenotype. The taste of 5 crop species, cucumber, pear, potato, strawberry, and tomato, was improved by expressing the thaumatin II gene under control of the 35S promoter. Only in the case of transgenic cucumber were sensory assessments carried out for several cultivation cycles. Also, processed fruits of transgenic cucumber, as well as fresh-cut transgenic cucumber salads, were the subject of sensory analysis. Transgenic cucumber expression of the thaumatin II gene resulted not only in sweeter taste, in comparison with the control, but also in higher aroma acceptability. Therefore, the authors of this article have summarized sensory properties of transgenic crops expressing the thaumatin II gene, placing special emphasis on the sensory characteristics of transgenic cucumber. Consumers often perceive disparagingly food deriving from genetically modified (GM) plants, partly because of some concerns about unintended effects. To address such concerns, this article also summarizes the food safety evaluation of transgenic crops expressing the thaumatin II gene. Future research directions are suggested.

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Agrobacterium-mediated transformation of Hyacinthus orientalis with thaumatin II gene to control fungal diseases

Cited by 21 publications

References 18 publications

Effect of Transgenesis on mRNA and miRNA Profiles in Cucumber Fruits Expressing Thaumatin II

Effect of Transgenesis on mRNA and miRNA Profiles in Cucumber Fruits Expressing Thaumatin II

A review on factors affecting the Agrobacterium-mediated genetic transformation in ornamental monocotyledonous geophytes

Genetically Modified Crops Expressing 35S‐Thaumatin II Transgene: Sensory Properties and Food Safety Aspects

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