Agrobacterium rhizogenes is a useful transporter for introducing T-DNA of the binary plasmid into the chrysanthemum, Dendranthema grandiflorum Kitamura, genome

Tsuro, Masato; Kubo, Takashi; Shizukawa, Yoshiaki; Takemoto, Takashi; Inaba, Koji

doi:10.1007/s11240-004-4783-1

Cited by 10 publications

(6 citation statements)

References 30 publications

(34 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In other studies, aberrant phenotype of tobacco plants (Dehio et al 1993), delayed senescence, shortened style (van Altvorst et al 1992), and reduced fertility (Sun et al 1991) have been reported in rolA transformants, while Osteosperum ecklonis rolA transformants produced profuse rooting with shorter branching and slightly earlier flowering (Giovannini et al 1999). Similarly, rolC transformants have shown dwarf phenotype with reduced apical dominance, early inflorescence, variation in leaf size and leaf number, and increased rooting ability in many plant species, including Rosa hybrida, Petunia hybrida, Pelargonium, Atropa belladonna, Osteospermum ecklonis, Malus micromalus, Chrysanthemum morifolium, and Nicotiana tabacum (for a review, see Casanova et al 2005;Tsuro et al 2005).…”

Section: Resultsmentioning

confidence: 98%

Expression of rol genes in transgenic soybean (Glycine max L.) leads to changes in plant phenotype, leaf morphology, and flowering time

Zia

Mirza

Malik

et al. 2010

Plant Cell Tiss Organ Cult

View full text Add to dashboard Cite

Soybean (Glycine max L.) cultivar NARC-4 was transformed with constructs carrying rolA, rolB, or rolC genes, each under the control of the Cauliflower Mosaic Virus 70S promoter. Cotyledonary nodes of soybean seeds were infected with Agrobacterium tumefaciens strain LBA4404 carrying one of the three rol genes, along with nptII in the plasmid pLBR. The efficiency of the transformation varied slightly among the three constructs, with frequencies of 6, 7, and 5% for the rolA, rolB, and rolC genes, respectively, being observed. Southern blot analysis confirmed the integration of rol genes in the soybean genome with varying numbers of copies of the transgene. All transformed plants showed enhanced rooting, but the number of adventitious roots was higher for transformants carrying the rolB transgene. rolA and rolC transformants showed dwarf phenotypes, clustered branching, and variations in leaf morphology. Furthermore, these plants flowered within a short period of time and produced lower numbers of flowers. rolB transformants showed variations in phenotype, including dwarf to semidwarf and shrubby growth, abnormal stem growth, short internodes, variations in leaf morphology, and greenish to yellowish-green colored leaves. These plants also flowered early, but dwarf plants produced low numbers of flowers, while shrubby plants produced high numbers of flowers, but these were mostly infertile.

show abstract

Section: Resultsmentioning

confidence: 98%

Expression of rol genes in transgenic soybean (Glycine max L.) leads to changes in plant phenotype, leaf morphology, and flowering time

Zia

Mirza

Malik

et al. 2010

Plant Cell Tiss Organ Cult

View full text Add to dashboard Cite

show abstract

“…Transformation was performed according to the method of Tsuro et al (2005). Putative transformants were screened for the presence of rolC genes by PCR analysis.…”

Section: Plant Transformationmentioning

confidence: 99%

Morphological and Physiological Changes in Transgenic Chrysanthemum morifolium Ramat. ‘Ogura-nishiki’ with rolC

崇¹,

正人²,

敦之³

et al. 2006

J. Japan. Soc. Hort. Sci.

Self Cite

View full text Add to dashboard Cite

The effects of rolC on morphogenesis of Chrysanthemum morifolium Ramat. 'Ogura-nishiki' were studied. The ray flowerets in the rolC-transformed plants were shorter than those in wild-type plants, but the transformation enhanced outgrowth of lateral shoots and reduced internode elongation. Studies on adventitious shoot and root formation from leaf segments revealed that the rolC transgene led to reduced root formation but enhanced adventitious bud formation. This enhanced cytokinin-like activity was consistent with phenotypes observed in the rolC-transformed plants.

show abstract

“…Why one plant without rolC regenerated from hairy roots is unclear. Tsuro et al (2005) found that wild-type A. rhizogenes (with pIG121-Hm) transported T-DNA of binary plasmid more efficiently than Ri-T-DNA alone in chrysanthemum. From our results and the above report, a transformant without rolC might have been regenerated from an adventitious root that included only T-DNA of the binary plasmid, not from a hairy root.…”

Section: Hairy Root Formation and Regenerationmentioning

confidence: 93%

“…It has been used to introduce binary plasmid T-DNA into plant genomes, e.g., petunia and melon (Kiyokawa et al, 1992;Toyoda et al, 1991). Tsuro et al (2005) suggested that…”

Section: Introductionmentioning

confidence: 99%

Efficient Genetic Transformation in Lavandin using Agrobacterium rhizogenes as Vector

Tsuro

Ikedo

Kato

2009

J. Japan. Soc. Hort. Sci.

Self Cite

View full text Add to dashboard Cite

To develop an efficient procedure for the genetic transformation of lavandin, wild-type Agrobacterium rhizogenes harboring pIG121-Hm was used for infection. β-Glucronidase (GUS) expression was compared in leaf segments and leaf-derived calli as inoculation explants after five days of cocultivation, but GUS expression was only observed on calli. Hairy roots with strong GUS expression formed in 27.3% of these calli. When root segments excised from hairy roots were cultured in media containing various concentrations of 6-benzylamino purine (BA), thidiazuron (TDZ), or N-(2-chloro-4-pyridyl)-N′-phenylurea (CPPU), adventitious shoots were formed in some media. The adventitious shoot formation rate depended on the plant-growth regulator, and the highest adventitious shoot formation rate was 77.5%, in medium containing 0.02 mg·L −1 CPPU. This medium was not as effective in the formation of adventitious shoots from calli. Shoots from hairy roots were easily rerooted and acclimatized in a temperature-and light-controlled room. Transformation efficiency was more than six times higher with the Agrobacterium rhizogenes-infected culture series (20.6%) than with the Agrobacterium tumefaciens-infected culture series (3.3%), in terms of the rates of hairy root induction and regeneration from hairy root segments. These results indicate that efficient genetic transformation in lavandin can be achieved with A. rhizogenes as the vector.

show abstract

Agrobacterium rhizogenes is a useful transporter for introducing T-DNA of the binary plasmid into the chrysanthemum, Dendranthema grandiflorum Kitamura, genome

Cited by 10 publications

References 30 publications

Expression of rol genes in transgenic soybean (Glycine max L.) leads to changes in plant phenotype, leaf morphology, and flowering time

Expression of rol genes in transgenic soybean (Glycine max L.) leads to changes in plant phenotype, leaf morphology, and flowering time

Morphological and Physiological Changes in Transgenic Chrysanthemum morifolium Ramat. ‘Ogura-nishiki’ with rolC

Efficient Genetic Transformation in Lavandin using Agrobacterium rhizogenes as Vector

Contact Info

Product

Resources

About