Expression of rol genes in transgenic soybean (Glycine max L.) leads to changes in plant phenotype, leaf morphology, and flowering time

Zia, Muhammad; Mirza, Bushra; Malik, Salman Akbar; Chaudhary, Muhammad Fayyaz

doi:10.1007/s11240-010-9771-z

Cited by 27 publications

(15 citation statements)

References 61 publications

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“…Consequently, it is not only necessary to develop new technologies to create germplasm resources but also breed new cultivars. Many reports showed that genetic transformation of plants with Agrobactrium rhizogenes can be used as an efficient way of breeding new cultivars for improvement and creation of germplasm resources (Zia et al 2010). So far, hairy roots which grows rapidly with stable and comparatively high content in secondary metabolites, induced by genetic transformation of A. rhizogenes, were successfully used for the production of essential oils from medicinal or aromatic plants, such as Rose-scented geranium (Pelargonium sp.)…”

Section: Introductionmentioning

confidence: 99%

“…So far, hairy roots which grows rapidly with stable and comparatively high content in secondary metabolites, induced by genetic transformation of A. rhizogenes, were successfully used for the production of essential oils from medicinal or aromatic plants, such as Rose-scented geranium (Pelargonium sp.) (Saxena et al 2007), Plumbago indica (Gangopadhyay et al 2010) and Psoralea drupacea (Fabaceae) (Lystvan et al 2010) and for improvement and creation of germplasm for Nierembergia scoparia (Godo et al1997), Kalanchoe blossfeldiana (Christensen et al 2008) and Glycine max (Zia et al 2010). The transgenic plants also produced from hairy roots are usually non-chimeric because the hairy roots originate from single cells and each hairy root consists of uniformly transformed cells.…”

Section: Introductionmentioning

confidence: 99%

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Induction of hairy roots and plant regeneration from the medicinal plant Pogostemon Cablin

Shi

Yong-Yue

Tie-Shan

et al. 2011

Plant Cell Tiss Organ Cult

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An efficient transformation system for the medicinal and aromatic plant, Pogostemon cablin Benth was developed by using agropine-type Agrobacterium rhizogenes ATCC15834. Hairy roots formed directly from the cut edges of leaf explants or via callus stage 8 days after inoculation with the bacterium. The highest frequency of leaf explant transformation by Agrobacterium rhizogenes ATCC15834 was about 80% after infection for 25 days. Hairy roots grew rapidly on plant growth regulators (PGRs)-free Murashige and Skoog (MS) or 6,7-V medium and had characteristics of transformed roots such as fast growth and high lateral branching. The PCR amplification showed that rol genes of Ri plasmid of A. rhizogenes were integrated and expressed into the genome of transformed hairy roots. The hairy root line, PL6, grew very slowly in the first 8 days, then grew very quickly between day 8 and day 24. The optimum medium for callus induction of hairy roots consisted of 2.0 mg l -1 benzyladenine (BA) and 0.1 mg/l a-naphthaleneacetic acid (NAA); while optimum medium for adventitious shoot regeneration from these cultures consisted of 0.1 mg l -1 BA and 0.1 mg l -1 NAA. Adventitious shoots could be rooted on 1/2MS. Southern blot analysis confirmed that rol genes of TL-DNA of Ri plasmid was integrated with at least three copies into the genome of hairy rootsregenerated P. cablin plants. The results presented provide a solid foundation for production of patchouli essential oil from hairy roots or its regenerated plants and also provide possibilities for utilization of artifical polyploidization or chemical mutation of hairy roots for improving germplasm and breeding of a new cultivar of P. cablin.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Induction of hairy roots and plant regeneration from the medicinal plant Pogostemon Cablin

Shi

Yong-Yue

Tie-Shan

et al. 2011

Plant Cell Tiss Organ Cult

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“…The development of transgenic Taxus cells needs an efficient genetic transformation method. The Agrobacterium-mediated transformation system has been commonly applied to engineer many plant species for desirable traits (Li et al 2008;Gelvin 2009;Raffeiner et al 2009;Silva et al 2009;Banyai et al 2010;Espasandin et al 2010;Ma et al 2010;Zia et al 2010). This system is favored over other methods, such as particle bombardment, due to the integration of low numbers of the transgene into the genome, the stability in the host cells, and the high transformation efficiency, etc.…”

Section: Introductionmentioning

confidence: 99%

Overexpression of a 10-deacetylbaccatin III-10 β-O-acetyltransferase gene leads to increased taxol yield in cells of Taxus chinensis

Zhang

Liu

et al. 2010

Plant Cell Tiss Organ Cult

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Taxus chinensis suspension cells were transformed by the Agrobacterium-mediated transformation method to overexpress the dbat gene coding for 10-deacetylbaccatin III-10 b-O-acetyltransferase, a key enzyme for taxol biosynthesis. A. tumefaciens strain LBA4404 harboring either pCAMBIA1303 or the recombinant plasmid p1303-SdbatN was used. Both plasmids harbored the hygromycin phosphotransferase gene (hptII) and gusAmgfp5 gene as selectable markers, but the latter plasmid also harbored the dbat gene in the T-DNA region. The transgenic T. chinensis cells had been maintained in modified Gamborg's B5 medium supplemented with hygromycin for more than 14 months and were subcultured at 4-week intervals. The selected transgenic cells were identified by PCR, Southern blot analysis, b-glucuronidase assay and western blot analysis, and the results showed that the transgenes were integrated in the chromosomal DNA of T. chinensis cells with single-copy style, and that the gusAmgfp5 reporter gene was expressed in the transgenic cells. The dbat mRNA expression level in the p1303-SdbatNtransgenic T. chinensis cells tested by real-time quantitative PCR was 5.3 ± 0.6 times that of the non-transformed cells. Taxol yield of the p1303-SdbatN-transgenic T. chinensis cells was about 1.7 times that of the non-transformed cells. These results suggest that the overexpression of dbat gene in transgenic T. chinensis cells leads to increased taxol yield.

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“…Many reports showed that genetic transformation of plants with Agrobactrium rhizogenes can be used as an efficient way of breeding new cultivars for improvement and creation of germplasm resources. 5 So far, hairy roots which grow rapidly with stable and comparatively high content in secondary metabolites, induced by genetic transformation of A. rhizogenes, were successfully used for the production of essential oils from medicinal or aromatic plants, such as Rose-scented geranium (Pelargonium sp. ), 6 Plumbago indica 7 and Psoralea drupacea (Fabaceae) 8 and for improvement and creation of germ-plasm for Nierembergia scoparia, 9 Kalanchoe blossfeldiana 10 and Glycine max.…”

Section: Introductionmentioning

confidence: 99%

“…), 6 Plumbago indica 7 and Psoralea drupacea (Fabaceae) 8 and for improvement and creation of germ-plasm for Nierembergia scoparia, 9 Kalanchoe blossfeldiana 10 and Glycine max. 5 The transgenic plants also produced from hairy roots are usually non-chimeric because the hairy roots originate from single cells and each hairy root consists of uniformly transformed cells. To date, there are only a few reports on breeding transgenic P. cablin plants with pest resistance by genetic transformation with A. tumefaciens.…”

Section: Introductionmentioning

confidence: 99%

Induction of hairy roots and plant regeneration from the medicinal plant Pogostemon Cablin

Yan¹,

He²,

Huang³

et al. 2015

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An efficient transformation system for the medicinal and aromatic plant, Pogostemon cablin Benth was developed by using Agrobacterium rhizogenes ATCC15834 and C58C1. Hairy roots formed directly from the cut edges of leaf explants after infection for 2 days. The highest frequency of leaf explant transformation by A. rhizogenes ATCC15834 and C58C1 were 83.3% and 80.5% after pre culture about 2 days and infection by the bacterium containing 15 mg l -1 acetosyringone about 25 min. The PCR amplification showed that rolB genes of Ri plasmid of A. rhizogenes were integrated and expressed into the genome of transformed hairy roots. The optimum medium for callus induction of hairy roots consisted of 2.0 mg l -1 BA and 0.1 mg l -1 NAA while optimum medium for adventitious shoot regeneration from these cultures consisted of 0.1 mg l -1 BA and 0.1 mg l -1 NAA. Adventitious shoots could be rooted on 1/2MS. PCR analysis confirmed that rol B gene of TL-DNA of Ri plasmid was integrated into the genome of hairy roots-regenerated P. cablin plants. The results presented provide a possibility for breeding of a new cultivar of P. cablin.

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Expression of rol genes in transgenic soybean (Glycine max L.) leads to changes in plant phenotype, leaf morphology, and flowering time

Cited by 27 publications

References 61 publications

Induction of hairy roots and plant regeneration from the medicinal plant Pogostemon Cablin

Induction of hairy roots and plant regeneration from the medicinal plant Pogostemon Cablin

Overexpression of a 10-deacetylbaccatin III-10 β-O-acetyltransferase gene leads to increased taxol yield in cells of Taxus chinensis

Induction of hairy roots and plant regeneration from the medicinal plant Pogostemon Cablin

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