Agrobacterium rhizogenes mediaredTransformation of Kiwifruit (Actinidia deliciosa) by Direct Formation of Adventitious Buds.

Yamakawa, Yoshihide; Chen, Li-Hung

doi:10.2503/jjshs.64.741

Cited by 15 publications

(3 citation statements)

References 30 publications

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“…Phenotype alterations were noted in some plants (Pe Ârez-Molphe-Balch and Ochoa-Alejo, 1998). In kiwifruit, Yamakawa and Chen (1996) achieved A. rhizogenes-mediated transformation by direct formation of adventitious buds on infected petioles. A mixture of normal and Ri phenotype plants were obtained.…”

Section: Normal Phenotypementioning

confidence: 97%

Use of ri-mediated transformation for production of transgenic plants

Christey

2001

In Vitro Cell.Dev.Biol.-Plant

169

109

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Agrobacterium rhizogenes-mediated transformation has been used to obtain transgenic plants in 89 different taxa, representing 79 species from 55 genera and 27 families. A diverse range of dicotyledonous plant families is represented, including one Gymnosperm family. In addition to the Ri plasmid, over half these plants have been transformed with foreign genes, including agronomically useful traits. Plants regenerated from hairy roots often show altered plant morphology such as dwarfing, increased rooting, altered flowering, wrinkled leaves and/or increased branching due to rol gene expression. These altered phenotypic features can have potential applications for plant improvement especially in the horticultural industry where such morphological alterations may be desirable. Use of A. rhizogenes and rol gene transformation has tremendous potential for genetic manipulation of plants and has been of particular benefit for improvement of ornamental and woody plants.

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Section: Normal Phenotypementioning

confidence: 97%

Use of ri-mediated transformation for production of transgenic plants

Christey

2001

In Vitro Cell.Dev.Biol.-Plant

169

109

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“…Previous studies have shown that A. rhizogenes NIASE1724 and ArM123 may also apply to kiwifruit A. deliciosa A. Chev. (Yazawa et al 1995 ; Yamakawa and Chen 1996 ) and their efficiency of hairy root induction ranged from 2.5 to 30%, respectively. In this study, we used a different strain K599 to infect ‘Hongyang’ and revealed that about 50% of explants produced hairy roots and each explant could generate an average of 3.3 hairy roots (Fig.…”

Section: Discussionmentioning

confidence: 99%

“…Recently, the cut-dip-budding delivery system using A. rhizogenes achieves effective transformation and gene editing without tissue culture and sterile conditions in the plant species with root-suckering ability (Cao et al 2023 ). Previous studies have shown that A. rhizogenes may also apply to kiwifruit (Yazawa et al 1995 ; Yamakawa and Chen 1996 ), but few studies utilizing hairy root transformation in kiwifruit have been reported, possibly because of its low transformation and regeneration efficiency.…”

Section: Introductionmentioning

confidence: 99%

Agrobacterium rhizogenes-mediated marker-free transformation and gene editing system revealed that AeCBL3 mediates the formation of calcium oxalate crystal in kiwifruit

Li,

Zhang,

Liang

et al. 2024

Mol Horticulture

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The transformation and gene editing of the woody species kiwifruit are difficult and time-consuming. The fast and marker-free genetic modification system for kiwifruit has not been developed yet. Here, we establish a rapid and efficient marker-free transformation and gene editing system mediated by Agrobacterium rhizogenes for kiwifruit. Moreover, a removing-root-tip method was developed to significantly increase the regeneration efficiency of transgenic hairy roots. Through A. rhizogenes-mediated CRISPR/Cas9 gene editing, the editing efficiencies of CEN4 and AeCBL3 achieved 55 and 50%, respectively. And several homozygous knockout lines for both genes were obtained. Our method has been successfully applied in the transformation of two different species of kiwifruit (Actinidia chinensis ‘Hongyang’ and A.eriantha ‘White’). Next, we used the method to study the formation of calcium oxalate (CaOx) crystals in kiwifruit. To date, little is known about how CaOx crystal is formed in plants. Our results indicated that AeCBL3 overexpression enhanced CaOx crystal formation, but its knockout via CRISPR/Cas9 significantly impaired crystal formation in kiwifruit. Together, we developed a fast maker-free transformation and highly efficient CRISPR-Cas9 gene editing system for kiwifruit. Moreover, our work revealed a novel gene mediating CaOx crystal formation and provided a clue to elaborate the underlying mechanisms. Graphical abstract

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Genetic Transformation and Fruit Crop Improvement

Singh¹,

Sansavini²

1998

Plant Breeding Reviews

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Agrobacterium rhizogenes mediaredTransformation of Kiwifruit (Actinidia deliciosa) by Direct Formation of Adventitious Buds.

Cited by 15 publications

References 30 publications

Use of ri-mediated transformation for production of transgenic plants

Use of ri-mediated transformation for production of transgenic plants

Agrobacterium rhizogenes-mediated marker-free transformation and gene editing system revealed that AeCBL3 mediates the formation of calcium oxalate crystal in kiwifruit

Genetic Transformation and Fruit Crop Improvement

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