Genetic Transformation and Fruit Crop Improvement

Singh, Zora; Sansavini, S.

doi:10.1002/9780470650110.ch3

Cited by 8 publications

(2 citation statements)

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“…Within the group of F&N species, the majority (over 95%) are still recalcitrant for transformation, and most of the transgenic F&N crops were produced using A . tumefaciens -mediated transformation (Singh and Sansavini, 1998; Kole and Hall, 2008). The scarce availability of regenerable explant sources and the time required to produce transgenic individuals are the major constraints (Figure 2).…”

Section: Transformation Protocols For Woody Fruit and Nut Cropsmentioning

confidence: 99%

“…Genetic transformation of F&N crops might be necessary when a breeding goal is not easily achievable through traditional breeding approaches (Singh and Sansavini, 1998). Of many approaches developed, gene addition and gene subtraction are two basic strategies for GM trait development (Pena and Seguin, 2001).…”

Section: Recent Developments In Genetic Engineering Of Fandn Cropsmentioning

confidence: 99%

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Agrobacterium-Mediated Transformation of Tree Fruit Crops: Methods, Progress, and Challenges

2019

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Genetic engineering based on Agrobacterium -mediated transformation has been a desirable tool to manipulate single or multiple genes of existing genotypes of woody fruit crops, for which conventional breeding is a difficult and lengthy process due to heterozygosity, sexual incompatibility, juvenility, or a lack of natural sources. To date, successful transformation has been reported for many fruit crops. We review the major progress in genetic transformation of these fruit crops made in the past 5 years, emphasizing reproducible transformation protocols as well as the strategies that have been tested in fruit crops. While direct transformation of scion cultivars was mostly used for fruit quality improvement, biotic and abiotic tolerance, and functional gene analysis, transgrafting on genetically modified (GM) rootstocks showed a potential to produce non-GM fruit products. More recently, genome editing technology has demonstrated a potential for gene(s) manipulation of several fruit crops. However, substantial efforts are still needed to produce plants from gene-edited cells, for which tremendous challenge remains in the context of either cell’s recalcitrance to regeneration or inefficient gene-editing due to their polyploidy. We propose that effective transient transformation and efficient regeneration are the key for future utilization of genome editing technologies for improvement of fruit crops.

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Section: Transformation Protocols For Woody Fruit and Nut Cropsmentioning

confidence: 99%

Section: Recent Developments In Genetic Engineering Of Fandn Cropsmentioning

confidence: 99%

Agrobacterium-Mediated Transformation of Tree Fruit Crops: Methods, Progress, and Challenges

2019

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Selection and Screening Strategies

Liang

Kumar²,

Nain³

et al. 2010

Transgenic Crop Plants

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Adventitious shoot regeneration from leaf explants of almond (Prunus dulcis mill.)

Ainsley

Collins

Sedgley

2000

In Vitro Cell.Dev.Biol.-Plant

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A method has been developed to facilitate shoot formation from leaf explants of almond. Leaves were dissected from micropropagated shoot cultures of the commercial cultivars Nonpareil and Ne Plus Ultra, and sections incubated on Almehdi and Parfitt's (1986) basal medium (AP) with varied plant growth-regulator conditions. Three auxins, 2,4-dichlorophenoxyacetic acid (2,4-D), a-naphthaleneacetic acid (NAA), and indole-3-butyric acid (IBA), in combination with two cytokinins, benzylaminopurine (BA) and thidiazuron (TDZ), were tested at various concentrations along with casein hydrolysate (CH) to determine the conditions most conducive to adventitious shoot regeneration. Response to the tested plant growth-regulator conditions varied with genotype. Of the three auxins tested, NAA and IBA induced adventitious shoots from Ne Plus Ultra explants, but only IBA was effective for Nonpareil. For the cytokinins, shoot development from Ne Plus Ultra occurred in the presence of either BA or TDZ, whereas for Nonpareil only TDZ was effective unless CH was incorporated in the basal medium. The inclusion of CH (0.1% w/v) improved callus morphology, and increased regeneration frequencies for both cultivars. Maximum regeneration frequencies for Ne Plus Ultra (44.4%) and Nonpareil (5.5%) were achieved on AP basal salts supplemented with CH, IBA (9.8 mM), and TDZ at 22.7 and 6.8 mM, respectively.

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Genetic Transformation and Fruit Crop Improvement

Cited by 8 publications

References 235 publications

Agrobacterium-Mediated Transformation of Tree Fruit Crops: Methods, Progress, and Challenges

Agrobacterium-Mediated Transformation of Tree Fruit Crops: Methods, Progress, and Challenges

Selection and Screening Strategies

Adventitious shoot regeneration from leaf explants of almond (Prunus dulcis mill.)

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