2019
DOI: 10.1371/journal.pone.0223419
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Agrobacterium tumefaciens-mediated transformation and expression of GFP in Ascochyta lentis to characterize ascochyta blight disease progression in lentil

Abstract: The plant immune system is made up of a complex response network that involves several lines of defense to fight invading pathogens. Fungal plant pathogens on the other hand, have evolved a range of ways to infect their host. The interaction between Ascochyta lentis and two lentil genotypes was explored to investigate the progression of ascochyta blight (AB) in lentils. In this study, we developed an Agrobacterium tumefaciens-mediated transformation system for A. lentis by constructing a new binary vector, pAT… Show more

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Cited by 3 publications
(18 citation statements)
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“…Ascochyta lentis Al Kewell, obtained from Kewell, Victoria [ 15 , 18 , 19 ] was grown in half strength potato dextrose agar (PDA, BD Difco, Sparks, MD, USA) supplemented with 30 µg mL −1 streptomycin (Astral Scientific, Gymea, Australia) at room temperature under a 12 h fluorescent light regime to induce sporulation. Maintenance and spore preparation of A. lentis isolates were performed as described by Davidson et al (2016) [ 15 ].…”
Section: Methodsmentioning
confidence: 99%
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“…Ascochyta lentis Al Kewell, obtained from Kewell, Victoria [ 15 , 18 , 19 ] was grown in half strength potato dextrose agar (PDA, BD Difco, Sparks, MD, USA) supplemented with 30 µg mL −1 streptomycin (Astral Scientific, Gymea, Australia) at room temperature under a 12 h fluorescent light regime to induce sporulation. Maintenance and spore preparation of A. lentis isolates were performed as described by Davidson et al (2016) [ 15 ].…”
Section: Methodsmentioning
confidence: 99%
“…The lentil accessions used in this study include the susceptible control ILL6002, resistant control ILL7537 together with Australian cultivars PBA Bolt, PBA Hurricane XT and Nipper. Lentil accessions PBA Hurricane XT and Nipper have been previously described [ 15 , 18 , 20 ] as susceptible and resistant to Al Kewell, respectively. Lentil varieties were obtained from Seednet (Horsham, Australia) and Pulse Breeding Australia (Horsham, Australia).…”
Section: Methodsmentioning
confidence: 99%
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