Three rod-shaped, Gram-stain-positive, and catalase-positive, phenotypically closely related isolates (HY052
T
, HY050, and HY045) were obtained from fecal samples collected from bats in Guangxi province and Chongqing city of China. Circular, smooth, light-yellow colonies appeared on brain heart infusion plate after 24–48 h incubation at 28°C. The optimal pH for growth was between 6.0 and 7.5. Based on 16S rRNA, the three isolates were phylogenetically related to
Agromyces terreus
DS-10
T
,
Agromyces aureus
AR33
T
,
Agromyces salentinus
20-5
T
,
Agromyces allii
UMS-62
T
,
Agromyces lapidis
CD55
T
, and
Agromyces italicus
CD1
T
. Moreover, based on 296 core genes, the phylogenomic tree indicated that the three isolates clustered together, closest to
Agromyces cerinus
VKM Ac-1340
T
and
Agromyces fucosus
VKM Ac-1345
T
but separated distantly from other
Agromyces
species. The average nucleotide identity values between strain HY052
T
and other
Agromyces
species ranged from 79.3% to 87.9%, lower than the 95–96% threshold. Furthermore, the genome of strain HY052
T
contains a circular chromosome of 3,437,203 bp with G + C content of 69.0 mol%. Main fatty acids were
anteiso
-C
15:0
and
anteiso
-C
17:0
. The polar lipids comprised diphosphatidylglycerol, phosphatidylglycerol, and unidentified glycolipids. Rhamnose, ribose, and glucose were the primary cell wall sugars. The major peptidoglycan amino acids included alanine, glutamic acid, glycine, and 2,4-diaminobutyric acid. An additional remarkable difference from other
Agromyces
species is that MK-12 was the sole menaquinone in strain HY052
T
. Based on results from the polyphasic characterizations performed in this study, our isolates are proposed to be members of a novel species in genus
Agromyces
, named
Agromyces laixinhei.
The type strain is HY052
T
(= CGMCC 1.17175
T
= JCM 33695
T
).
Electronic Supplementary Material
Supplementary material is available in the online version of this article at 10.1007/s12275-021-0546-5.