AHAS Trp574Leu substitution in <i>Raphanus sativus</i> L.: screening, enzyme activity and fitness cost

Vercellino, Román Boris; Pandolfo, Claudio Ezequiel; Breccia, Gabriela; Cantamutto, Miguel Ángel; Presotto, Alejandro

doi:10.1002/ps.4849

Cited by 24 publications

(71 citation statements)

References 35 publications

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“…In a previous study, AHAS-resistant feral radish carrying the Trp574Leu mutation showed negative pleiotropic effects on AHAS activity and reproductive traits under intraspecific interference 19 . However, the lack of information of fitness costs associated with the Trp574Leu mutation in different life-history traits of feral radish and under crop interference limits the development and implementation of evolution-knowledge-based resistance management strategies .…”

Section: Introductionmentioning

confidence: 84%

Section: Plant Materialsmentioning

confidence: 99%

“…The first AHAS-resistant feral R. sativus population reported in Argentina was used in this study 2,19,22 . Recently evolved AHAS-resistant plants carrying the Trp574Leu mutation were collected in the south of Buenos Aires province, in a field planted with IMI-resistant oilseed rape after imazethapyr application by the farmer 19,22 . From these plants, a purified homozygous sub-population with the Trp574Leu mutation (hereafter R biotype) was generated by selecting at least 15 homozygous resistant plants using a specific Trp574Leu mutation CAPS marker, following the procedure described in Pandolfo et al 17 .…”

Section: Plant Materialsmentioning

confidence: 99%

“…Feral radish is a widespread problematic winter weed in temperate zones of the Americas that has developed resistance to AHAS herbicides in Brazil, Chile and Argentina 2,17 . In Argentina, feral radish is one of the most noxious weeds in the south of Buenos Aires province, the main winter cereal producing area 18 , and the Trp574Leu mutation has been identified in several AHAS-resistant populations 17,19 .…”

Section: Introductionmentioning

confidence: 99%

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Ecological fitness cost associated with the AHAS Trp574Leu mutation in feralRaphanus sativus

Vercellino

Hernández

Pandolfo

et al. 2020

Preprint

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BACKGROUNDGene mutations endowing herbicide resistance may have negative pleiotropic effects on plant fitness. Quantifying these effects is critical for predicting the evolution of herbicide resistance and developing management strategies for herbicide-resistant weeds. This study reports the effects of the acetohydroxyacid synthase (AHAS) Trp574Leu mutation throughout the life cycle of the weed feral radish (Raphanus sativus L.).RESULTSResistant and susceptible biotypes responded differently to light and water treatments in relation to germination and emergence. Under light exposure, the resistant biotype showed higher germination and emergence, but no differences were found in seed dormancy, germination in darkness and emergence from buried seeds or pods. The resistant biotype showed delayed and reduced seedling emergence relative to the susceptible biotype under rainfed conditions, but these differences between the biotypes were not detected in irrigated soil. The biotypes showed similar relative growth rates and vegetative biomass. However, under wheat interference, resistant plants had 36–46% less total above-ground biomass, 26–47% less seeds per plant, and 36–53% less plant yield than susceptible ones, and these differences were more evident at higher plant density.CONCLUSIONThis study provides a better understanding of the ecological fitness cost associated with the AHAS Trp574Leu mutation in feral R. sativus. The fitness costs could reduce the frequency of the resistant allele in areas untreated with AHAS inhibiting herbicides.

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Section: Introductionmentioning

confidence: 84%

Section: Plant Materialsmentioning

confidence: 99%

Section: Plant Materialsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Ecological fitness cost associated with the AHAS Trp574Leu mutation in feralRaphanus sativus

Vercellino

Hernández

Pandolfo

et al. 2020

Preprint

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“…). Several works showed that herbicide resistance‐endowing AHAS gene mutations result in decreased AHAS activity in dicots . Increased or unchanged AHAS activity has been observed for a number of resistance mutations in other species .…”

Section: Discussionmentioning

confidence: 99%

Effect of Ahasl1‐1 and Ahasl1‐4 alleles on herbicide resistance and its associated dominance in sunflower

Breccia

Gianotto

Altieri³

et al. 2018

Pest Management Science

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The role of intraspecific crop–weed hybridization in the evolution of weediness and invasiveness: Cultivated and weedy radish (Raphanus sativus) as a case study

Vercellino,

Hernández,

Presotto

2023

American J of Botany

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PremiseThe phenotype of hybrids between a crop and its wild or weed counterpart is usually intermediate and maladapted compared to that of their parents; however, hybridization has sometimes been associated with increased fitness, potentially leading to enhanced weediness and invasiveness. Since the ecological context and maternal genetic effects may affect hybrid fitness, they could influence the evolutionary outcomes of hybridization. Here, we evaluated the performance of first‐generation crop–weed hybrids of Raphanus sativus and their parents in two contrasting ecological conditions.MethodsUsing experimental hybridization and outdoor common garden experiments, we assessed differences in time to flowering, survival to maturity, plant biomass, and reproductive components between bidirectional crop–weed hybrids and their parents in agrestal (wheat cultivation, fertilization, weeding) and ruderal (human‐disturbed, uncultivated area) conditions over 2 years.ResultsCrop, weeds, and bidirectional hybrids overlapped at least partially during the flowering period, indicating a high probability of gene flow. Hybrids survived to maturity at rates at least as successful as their parents and had higher plant biomass and fecundity, which resulted in higher fitness compared to their parents in both environments, without any differences associated with the direction of the hybridization.ConclusionsIntraspecific crop–weed hybridization, regardless of the cross direction, has the potential to promote weediness in weedy R. sativus in agrestal and ruderal environments, increasing the chances for introgression of crop alleles into weed populations. This is the first report of intraspecific crop–weed hybridization in R. sativus.

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AHAS Trp574Leu substitution in Raphanus sativus L.: screening, enzyme activity and fitness cost

Abstract: This is the first report of fitness cost associated with the AHAS Trp574Leu mutation in R. sativus populations. This fitness cost could reduce frequency of the resistant allele without herbicide selection. © 2018 Society of Chemical Industry.

Cited by 24 publications

References 35 publications

Ecological fitness cost associated with the AHAS Trp574Leu mutation in feralRaphanus sativus

Ecological fitness cost associated with the AHAS Trp574Leu mutation in feralRaphanus sativus

Effect of Ahasl1‐1 and Ahasl1‐4 alleles on herbicide resistance and its associated dominance in sunflower

The role of intraspecific crop–weed hybridization in the evolution of weediness and invasiveness: Cultivated and weedy radish (Raphanus sativus) as a case study

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