Air-Adapted Methanosarcina acetivorans Shows High Methane Production and Develops Resistance against Oxygen Stress

Jasso‐Chávez, Ricardo; Santiago-Martínez, Michel Geovanni; Lira-Silva, Elizabeth; Pineda, Erika; Zepeda-Rodríguez, Armando; Belmont-Díaz, Javier A.; Encalada, Rusely; Saavedra, Emma; Moreno‐Sánchez, Rafael

doi:10.1371/journal.pone.0117331

Cited by 39 publications

(29 citation statements)

References 53 publications

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“…). It is possible that perhaps this taxa has unique antioxidant strategies for tolerating oxygen fluctuations, as has been suggested for other methanogens (Tholen et al ., ; Angel et al ., ; Jasso‐Chávez et al ., ). Similarly, while examining the methanotrophic community, we also identified OTUs from the bacterial Methanococcales family.…”

Section: Discussionmentioning

confidence: 97%

High‐resolution sequencing reveals unexplored archaeal diversity in freshwater wetland soils

Narrowe

Angle

Daly

et al. 2017

Environmental Microbiology

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Despite being key contributors to biogeochemical processes, archaea are frequently outnumbered by bacteria, and consequently are underrepresented in combined molecular surveys. Here, we demonstrate an approach to concurrently survey the archaea alongside the bacteria with high-resolution 16S rRNA gene sequencing, linking these community data to geochemical parameters. We applied this integrated analysis to hydric soils sampled across a model methane-emitting freshwater wetland. Geochemical profiles, archaeal communities, and bacterial communities were independently correlated with soil depth and water cover. Centimeters of soil depth and corresponding geochemical shifts consistently affected microbial community structure more than hundreds of meters of lateral distance. Methanogens with diverse metabolisms were detected across the wetland, but displayed surprising OTU-level partitioning by depth. Candidatus Methanoperedens spp. archaea thought to perform anaerobic oxidation of methane linked to iron reduction were abundant. Domain-specific sequencing also revealed unexpectedly diverse non-methane-cycling archaeal members. OTUs within the underexplored Woesearchaeota and Bathyarchaeota were prevalent across the wetland, with subgroups and individual OTUs exhibiting distinct occupancy and abundance distributions aligned with environmental gradients. This study adds to our understanding of ecological range for key archaeal taxa in a model freshwater wetland, and links these taxa and individual OTUs to hypotheses about processes governing biogeochemical cycling.

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Section: Discussionmentioning

confidence: 97%

High‐resolution sequencing reveals unexplored archaeal diversity in freshwater wetland soils

Narrowe

Angle

Daly

et al. 2017

Environmental Microbiology

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“…Archaeal lipids are characterized by the presence of isoprenoid side chains linked to an sn -glycerol-1-phosphate moiety by an ether linkage ( Albers and Meyer, 2011 ). Though the lipids of archaea differ from bacterial and eukaryotic lipids, MDA is a common indicator of lipid peroxidation even in archaea ( Jasso-Chávez et al, 2015 ). All the four haloarchaea showed increased production of MDA content after 24 h of treatment with SNP.…”

Section: Discussionmentioning

confidence: 99%

Antimicrobial Activity and Mechanism of Inhibition of Silver Nanoparticles against Extreme Halophilic Archaea

Thombre¹,

Shinde²,

Thaiparambil³

et al. 2016

Front. Microbiol.

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Haloarchaea are salt-loving halophilic microorganisms that inhabit marine environments, sea water, salterns, and lakes. The resistance of haloarchaea to physical extremities that challenge organismic survival is ubiquitous. Metal and antibiotic resistance of haloarchaea has been on an upsurge due to the exposure of these organisms to metal sinks and drug resistance genes augmented in their natural habitats due to anthropogenic activities and environmental pollution. The efficacy of silver nanoparticles (SNPs) as a potent and broad spectrum inhibitory agent is known, however, there are no reports on the inhibitory activity of SNPs against haloarchaea. In the present study, we have investigated the antimicrobial potentials of SNPs synthesized using aqueous leaf extract of Cinnamomum tamala against antibiotic resistant haloarchaeal isolates Haloferax prahovense RR8, Haloferax lucentense RR15, Haloarcula argentinensis RR10 and Haloarcula tradensis RR13. The synthesized SNPs were characterized by UV-Vis spectroscopy, scanning electron microscopy, energy dispersive X-ray spectroscopy, dynamic light scattering, X-ray diffraction and Fourier transform infrared spectroscopy. The SNPs demonstrated potent antimicrobial activity against the haloarchaea with a minimum inhibitory concentration of 300–400 μg/ml. Growth kinetics of haloarchaea in the presence of SNPs was studied by employing the Baranyi mathematical model for microbial growth using the DMFit curve fitting program. The C. tamala SNPs also demonstrated cytotoxic activity against human lung adenocarcinoma epithelial cell line (A540) and human breast adenocarcinoma cell line (MCF-7). The mechanism of inhibition of haloarchaea by the SNPs was investigated. The plausible mechanism proposed is the alterations and disruption of haloarchaeal membrane permeability by turbulence, inhibition of respiratory dehydrogenases and lipid peroxidation causing cellular and DNA damage resulting in cell death.

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“…Methanogenesis can also be activated by wetting of arid soils, whereby acetoclastic/hydrogenotrophic Methanosarcina and hydrogenotrophic Methanocella organisms consume fermentative end products produced by heterotrophs as substrates for methane production (78,83,84). Chemolithoautotrophs are also known to accumulate energy reserves when under stressed conditions (85)(86)(87)(88)(89).…”

Section: Energy Reserve Hypothesismentioning

confidence: 99%

Energetic Basis of Microbial Growth and Persistence in Desert Ecosystems

et al. 2020

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Microbial life is surprisingly abundant and diverse in global desert ecosystems. In these environments, microorganisms endure a multitude of physicochemical stresses, including low water potential, carbon and nitrogen starvation, and extreme temperatures. In this review, we summarize our current understanding of the energetic mechanisms and trophic dynamics that underpin microbial function in desert ecosystems. Accumulating evidence suggests that dormancy is a common strategy that facilitates microbial survival in response to water and carbon limitation. Whereas photoautotrophs are restricted to specific niches in extreme deserts, metabolically versatile heterotrophs persist even in the hyper-arid topsoils of the Atacama Desert and Antarctica. At least three distinct strategies appear to allow such microorganisms to conserve energy in these oligotrophic environments: degradation of organic energy reserves, rhodopsin- and bacteriochlorophyll-dependent light harvesting, and oxidation of the atmospheric trace gases hydrogen and carbon monoxide. In turn, these principles are relevant for understanding the composition, functionality, and resilience of desert ecosystems, as well as predicting responses to the growing problem of desertification.

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Air-Adapted Methanosarcina acetivorans Shows High Methane Production and Develops Resistance against Oxygen Stress

Cited by 39 publications

References 53 publications

High‐resolution sequencing reveals unexplored archaeal diversity in freshwater wetland soils

High‐resolution sequencing reveals unexplored archaeal diversity in freshwater wetland soils

Antimicrobial Activity and Mechanism of Inhibition of Silver Nanoparticles against Extreme Halophilic Archaea

Energetic Basis of Microbial Growth and Persistence in Desert Ecosystems

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